Chemical and electrochemical oxidation of aqueous solutions of NADH and model compounds

Abstract

International audienceAqueous solutions of NADH and of model compounds such as 1-4,dihydro-N1-propyl- and 1-4,dihydro-N1-benzyl-nicotinamide are oxidized on rotating Pt disk electrode at +0.66 ± 0.02 V (N.H.E.). This two-electron wave is pH independent in the range of 7-13. Both macroelectrolysis on Pt at +0.85 V (N.H.E.) and chemical oxidation by [Fe (CN)6]3- lead to NAD+ or model compounds. Differences between biochemical, chemical and electrochemical oxidation of NADH are discussed, especially in terms of energetics and mechanisms of reactions

Detection of microbial cells by cyclic voltammetry

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Electrocatalytic Oxidation of Dihydronicotinamide Adenine Dinucleotide with Ferrocene Carboxylic Acid by Diaphorase fromClostridium kluveri. Remarks on the Kinetic Approaches Usually Adopted

“…Taking into account the systems described by Equations 2, 3a and 3b, we generated via DigiSim algorithm voltammetric curves for various concentration ratios and rate constants and treated the data so obtained by the Cass procedure. Figure 3 shows the plots of k cat yaa FvyRT vs. 1yv for ratios of substrateymediator concentration equal to 20 : 1, 50 : 1, 100 : 1 and 500 : 1 and for k 2 …”

Stability of catechol modified carbon electrodes for electrocatalysis of dihydronicotinamide adenine dinucleotide and ascorbic acid

“…Making no attempt to be exhaustive, we may note in a first group the null current methods (5,6) generally applied with platinum electrodes; the measurements are always performed in the presence of oxidoreduction mediators (7). In a second group of methods the titrating mediator of the biological molecule is generated by coulometry (8,9).…”