“…The optical density (O.D) was taken at 600 nm with intervals of 0, 2, 4, 6, 8, 10, 12, 14 and 15 hours using spectrophotometer. Physical growth factors as temperature, pH, Inoculums volume, minimum inhibitory concentration (MIC) were studied by growing strains on different temperatures (25˚C, 30˚C, 37˚C, 42˚C, 56˚C and 57˚C), pH (5,6,7,8,9,10), Inoculums volume (125 µl, 250 µl, 500 µl), MIC (50 µg•ml …”