Pyranocoumarins Isolated from Peucedanum praeruptorum Dunn Suppress Lipopolysaccharide-Induced Inflammatory Response in Murine Macrophages Through Inhibition of NF-κB and STAT3 Activation

Abstract

Praeruptorin C, D, and E (PC, PD, and PE) are three pyranocoumarins isolated from the dried root of Peucedanum praeruptorum Dunn of Umbelliferae. In the present study, we investigated the anti-inflammatory effect of these compounds in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Pyranocoumarins significantly inhibited LPS-induced production of nitric oxide, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). The mRNA and protein expressions of inducible nitric oxide synthase, IL-6, and TNF-α were also suppressed by these compounds. Both PD and PE exhibited greater anti-inflammatory activities than PC. Further study showed that pyranocoumarins suppressed the cytoplasmic loss of inhibitor κB-α protein and inhibited the translocation of NF-κB from cytoplasm to nucleus. In addition, pyranocoumarins suppressed LPS-induced STAT3 tyrosine phosphorylation. Taken together, the results suggest that pyranocoumarins may exert anti-inflammatory effects in LPS-stimulated RAW 264.7 macrophages through the inhibition of NF-κB and STAT3 activation.

Mollugin Inhibits the Inflammatory Response in Lipopolysaccharide-Stimulated RAW264.7 Macrophages by Blocking the Janus Kinase-Signal Transducers and Activators of Transcription Signaling Pathway

“…To investigate whether the inhibition of inflammatory response by mollugin is mediated through the MAPKs pathway, MAPKs phosphorylation was examined by Western blot in RAW264.7 cells pretreated with mollugin and then with LPS according to the published method. 16) As shown in Fig. 4, mollugin did not suppress the lPS-stimulated phosphorylation of ErK1/2, p38, and JNK1/2.…”

Pyranocoumarins from Root Extracts of Peucedanum praeruptorum Dunn with Multidrug Resistance Reversal and Anti-Inflammatory Activities

“…Phytochemical and pharmacological studies showed that angular-type pyranocoumarins are the major constituents of this plant [3][4][5][6][7][8][9], and these compounds have various beneficial effects such as anti-inflammatory [10][11][12], antiasthma [13], chemopreventive [14], smooth muscle relaxant [15], neuroprotective [16], and anti-osteoclastogenic properties [17].…”

“…The structures of the isolates were determined spectroscopically using one-dimensional (1D)-and two-dimensional (2D)-nuclear magnetic resonance (NMR) analysis. All the compounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16) were evaluated for multidrug resistance (MDR) reversal and anti-inflammatory activity against multidrug resistant MES-SA/Dx5 cancer and lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Here, we 1 Hz, H-4′′′), 1.81 (3H, m, H-5′′′)] were observed from the 1 H-NMR spectrum.…”

“…praeruptorum roots and the constituents have been reported to have modulatory effects on tumor cells such as chemopreventive [14], anti-inflammatory [10][11][12], and multidrug resistance reversal [41,42]. Therefore, we examined the biological activities of the compounds isolated from the root extracts of P. praeruptorum using several in vitro assays to evaluate various aspects of their potential anticancer properties.…”

The anti-inflammatory activity of several flavonoids isolated fromMurraya paniculataon murine macrophage cell line and gastric epithelial cell (GES-1)

“…Macrophage is a type of immune cells that plays a critical role in immune responses, host defense, and tissue repair. This cell type produces various pro-inflammatory mediators such as tumor necrosis factor-alpha (TNF-a) and IL-6, as well as several inflammatory mediators such as NO and prostaglandin E 2 (PGE 2 ) when treated with LPS (Yu et al 2012). RAW 264.7 cell line has been demonstrated as a good model to conduct studies on inflammatory responses.…”