Pyramiding of four resistance genes of bacterial blight in Tapaswini, an elite rice cultivar, through marker-assisted selection

Dokku, Prasad; Das, Kajal; Rao, G. J. N.

doi:10.1007/s10681-013-0878-2

Cited by 72 publications

(102 citation statements)

References 18 publications

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“…Marker-assisted selection has been successful for marker-assisted backcrossing (Kim et al, 2010a,b;Diers, 2009, 2013) and pyramiding of major QTL (Lee, 1995;Liu et al, 2000;Werner et al, 2005;Dokku et al, 2013) for various crop species. Marker-assisted selection has been successful for marker-assisted backcrossing (Kim et al, 2010a,b;Diers, 2009, 2013) and pyramiding of major QTL (Lee, 1995;Liu et al, 2000;Werner et al, 2005;Dokku et al, 2013) for various crop species.…”

Section: Applications In Molecular Breeding and Genomic Selectionmentioning

confidence: 99%

“…Across crop species there have been many QTL for various traits reported; however, few of these QTL are ever reported as being used directly or intentionally within breeding programs. Marker-assisted selection has been successful for marker-assisted backcrossing (Kim et al, 2010a,b;Diers, 2009, 2013) and pyramiding of major QTL (Lee, 1995;Liu et al, 2000;Werner et al, 2005;Dokku et al, 2013) for various crop species. For oat, molecular markers have been used for marker-assisted backcrossing of various disease resistance genes (Rines et al, 2007;Gnanesh et al, 2013).…”

Section: Applications In Molecular Breeding and Genomic Selectionmentioning

confidence: 99%

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Improvement of Oat as a Winter Forage Crop in the Southern United States

2014

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Oat (Avena sativa L.) is a cool season annual grass species produced for grain and forage in many countries. The majority of oat cultivars in the United States are spring types grown primarily for grain production. However, in the southern region of the United States, much of the oat production consists of winter types that are grown for grain or animal forage, and in some cases as a dual‐purpose crop. As with other small grain crops used for forage production, the improvement of grazing tolerance, forage yield, and forage quality have not been major goals in U.S. oat breeding programs even though oat forage is a rich source of protein, vitamin B1, phosphorus, iron, and other minerals. However, breeding and research efforts for oat have recently been revitalized because of increased awareness of the positive health benefits associated with oat consumed as a whole grain food. Strengthening the molecular approaches for forage oat breeding in the southern United States could have a large impact on cattle production systems and could increase the production area planted to oat. Although many of the molecular tools developed for the improvement of grain production could be applicable to winter forage oat breeding, the tools are currently an untapped resource within the forage breeding community. The objectives of this manuscript are to examine the production of forage oat, the current state of forage oat breeding, and how molecular tools could aid and strengthen the development of improved forage oat cultivars.

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Section: Applications In Molecular Breeding and Genomic Selectionmentioning

confidence: 99%

Section: Applications In Molecular Breeding and Genomic Selectionmentioning

confidence: 99%

Improvement of Oat as a Winter Forage Crop in the Southern United States

2014

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“…In particular R -gene pyramiding is a useful method to maximize the use of known R -genes, which appear to durable and broad-spectrum resistance to various races or isolates of a specific pathogen (Kim et al 2018). This R -gene combining approach with three or four genes were reported in various case of rice resistance breeding studies (Singh et al 2001; Sundaram et al 2008; Dokku et al 2013; Suh et al 2013; Pradhan et al 2015). …”

Section: Introductionmentioning

confidence: 86%

Identification of novel recessive gene xa44(t) conferring resistance to bacterial blight races in rice by QTL linkage analysis using an SNP chip

Kim

2018

Theor Appl Genet

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Key messageUsing QTL analysis and fine mapping, the novel recessive gene xa44(t) conferring resistance to BB was identified and the expression level of the gene was confirmed through qRT-PCR analysis.AbstractBacterial blight (BB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo) is a major factor causing rice yield loss in most rice-cultivating countries, especially in Asia. The deployment of cultivars with resistance to BB is the most effective method to control the disease. However, the evolution of new Xoo or pathotypes altered by single-gene-dependent mutations often results in breakdown of resistance. Thus, efforts to identify novel R-genes with sustainable BB resistance are urgently needed. In this study, we identified three quantitative trait loci (QTLs) on chromosomes 1, 4, and 11, from an F2 population of 493 individuals derived from a cross between IR73571-3B-11-3-K3 and Ilpum using a 7K SNP chip. Of these QTLs, one major QTL, qBB_11, on chromosome 11 explained 61.58% of the total phenotypic variance in the population, with an LOD value of 113.59, based on SNPs 11964077 and 11985463. The single major R-gene, with recessive gene action, was designated xa44(t) and was narrowed down to a 120-kb segment flanked within 28.00 Mbp to 28.12 Mbp. Of nine ORFs present in the target region, two ORFs revealed significantly different expression levels of the candidate genes. These candidate genes (Os11g0690066 and Os11g0690466) are described as “serine/threonine protein kinase domain containing protein” and “hypothetical protein,” respectively. The results will be useful to further understand BB resistance mechanisms and provide new sources of resistance, together with DNA markers for MAS breeding to improve BB resistance in rice.Electronic supplementary materialThe online version of this article (10.1007/s00122-018-3187-2) contains supplementary material, which is available to authorized users.

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“…Rice ( Oryza sativa L.) is the second largest cereal crop and an essential food crop for billions of people and it is grown under various environmental conditions (Dokku, Das, & Rao, ). The methods for establishing the crop are principally divided into transplanting and direct seeding according to distinct and salient features.…”

Section: Introductionmentioning

confidence: 99%

Marker‐assisted breeding for improvement of anaerobic germination in japonica rice (Oryza sativa)

Kim

Jeong

et al. 2019

Plant Breeding

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Direct seeding is increasingly being practiced in rice cultivation areas because it saves labour and reduces cost of production and management. In this study, four japonica‐type breeding lines were developed from a cross of a japonica cultivar and an AG‐tolerant donor, KHO, using marker‐assisted selection. Validation of the target genes AG1 and AG2 in the selected lines was examined by PCR analysis and the background recovery rate by the recurrent parent was confirmed by genotyping using a 6K SNP chip. Compared to the recurrent, the survival rates of the selected lines were improved in a range from 33% to 115% in three conditions; in particular, the survival rate was increased in more than twice that of the recurrent parent under the hypoxic condition. The main agronomic trait, yield and quality‐related traits were similar to those of the recurrent parent. The use of these lines will contribute to the expansion of the cultivation area due to the proven economic benefits of direct seeding in Korea.

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Pyramiding of four resistance genes of bacterial blight in Tapaswini, an elite rice cultivar, through marker-assisted selection

Cited by 72 publications

References 18 publications

Improvement of Oat as a Winter Forage Crop in the Southern United States

Improvement of Oat as a Winter Forage Crop in the Southern United States

Identification of novel recessive gene xa44(t) conferring resistance to bacterial blight races in rice by QTL linkage analysis using an SNP chip

Marker‐assisted breeding for improvement of anaerobic germination in japonica rice (Oryza sativa)

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