Pyramiding and evaluation of the brown planthopper resistance genes Bph14 and Bph15 in hybrid rice

Hu, Jie; X, Li; Wu, Changjun; Yang, Chaolong; Hua, Hongxia; Gao, Guanjun; Xiao, Jinghua; He, Yuqing

doi:10.1007/s11032-010-9526-x

Cited by 75 publications

(70 citation statements)

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“…Resistance genes BPH14 and BPH15 were introgressed from wild rice Oryza officinalis [2]. These two genes showed significant resistance to BPH and have been broadly employed in rice breeding programs [3, 4]. Recently, BPH14 was isolated using a map-based cloning strategy, and it was found to encode a coiled-coil, nucleotide-binding, and leucine-rich repeat (CC-NB-LRR) protein that activates the SA signaling pathway [5].…”

Section: Introductionmentioning

confidence: 99%

BAC and RNA sequencing reveal the brown planthopper resistance gene BPH15 in a recombination cold spot that mediates a unique defense mechanism

Shangguan

et al. 2014

BMC Genomics

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BackgroundBrown planthopper (BPH, Nilaparvata lugens Stål), is the most destructive phloem-feeding insect pest of rice (Oryza sativa). The BPH-resistance gene BPH15 has been proved to be effective in controlling the pest and widely applied in rice breeding programs. Nevertheless, molecular mechanism of the resistance remain unclear. In this study, we narrowed down the position of BPH15 on chromosome 4 and investigated the transcriptome of BPH15 rice after BPH attacked.ResultsWe analyzed 13,000 BC2F2 plants of cross between susceptible rice TN1 and the recombinant inbred line RI93 that carrying the BPH15 gene from original resistant donor B5. BPH15 was mapped to a 0.0269 cM region on chromosome 4, which is 210-kb in the reference genome of Nipponbare. Sequencing bacterial artificial chromosome (BAC) clones that span the BPH15 region revealed that the physical size of BPH15 region in resistant rice B5 is 580-kb, much bigger than the corresponding region in the reference genome of Nipponbare. There were 87 predicted genes in the BPH15 region in resistant rice. The expression profiles of predicted genes were analyzed. Four jacalin-related lectin proteins genes and one LRR protein gene were found constitutively expressed in resistant parent and considered the candidate genes of BPH15. The transcriptomes of resistant BPH15 introgression line and the susceptible recipient line were analyzed using high-throughput RNA sequencing. In total, 2,914 differentially expressed genes (DEGs) were identified. BPH-responsive transcript profiles were distinct between resistant and susceptible plants and between the early stage (6 h after infestation, HAI) and late stage (48 HAI). The key defense mechanism was related to jasmonate signaling, ethylene signaling, receptor kinase, MAPK cascades, Ca2+ signaling, PR genes, transcription factors, and protein posttranslational modifications.ConclusionsOur work combined BAC and RNA sequencing to identify candidate genes of BPH15 and revealed the resistance mechanism that it mediated. These results increase our understanding of plant–insect interactions and can be used to protect against this destructive agricultural pest.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-674) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

BAC and RNA sequencing reveal the brown planthopper resistance gene BPH15 in a recombination cold spot that mediates a unique defense mechanism

Shangguan

et al. 2014

BMC Genomics

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“…Galur-galur padi yang mengandung piramida gen ketahanan Bph masih dalam tahap pengembangan. Contohnya, galur yang mengandung Bph1 dan bph2 (Sharma et al 2004), Bph6 dan Bph12 (Qiu et al 2012), Bph14 dan Bph15 (Hu et al 2012), Bph14, Bph15, dan Bph18 (Hu et al 2013), dan Bph3 dan Bph27(t) (Liu et al 2016) menunjukkan ketahanan antibiosis, antixenosis, ataupun toleransi yang lebih tinggi dibanding dengan gen Bph tunggal. Galur pemulih dengan toleransi terhadap tiga hama sekaligus (WBC, penggerek batang, dan penggulung daun) dan herbisida glufosinat dengan akumulasi Bph14 dan Bph15, gen Cry1C, dan gen bar telah dikembangkan oleh Wan et al (2014).…”

Section: Strategi Penghambatan Laju Adaptasi Virulensi Wbcunclassified

Virulensi Wereng Batang Cokelat (Nilaparvata lugens Stål) dan Strategi Pengelolaannya

Chaerani¹

2018

J. AgroBiogen

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Brown planthopper (BPH) emerged as serious pest of rice in Asia as a result of green revolution. BPH outbreak is supported by its monofagous feeding behavior on rice, high fecundity, and ability to long distance migration. BPH is labile in virulence and capable of rapid adaptation on new resistant varieties. The terminology 'biotype' to describe BPH virulence is still debatable because of individual virulence and genetic variation within a biotype. This paper reviews the biology, biochemistry, interaction with endosymbionts, and genetics aspects of BPH to reveal virulence basis of BPH for improvement of BPH management strategies. At the molecular level, differential expression profiles of genes related to digestion, saliva secretion, detoxification, metabolisms of lipid, carbohydrate, amino acid, and nitrogen; signaling pathways of defense, stress, and immunity responses were noted among BPH population with differential virulence. Research in genomics revealed complementary interaction between BPH and endosymbiotic microorganisms, but experiments using aposymbiotic BPH showed that endosymbiont densities do not always positively correlated with BPH virulence. In contrast to the previous finding, recent genetic mapping of BPH Avr gene using inbred populations revealed that BPH virulence is monogenically controlled. With this finding, a gene-for-gene relationship between BPH and its host can been established. Strategies for utilizing resistant varieties to anticipate virulence adaptation according to the dynamics of resistant rice varieties deployed in the field are discussed in this paper.Keywords: Brown planthopper, virulence, biotype, adaptation, endosymbiont. ABSTRAKWereng batang cokelat (WBC) timbul menjadi hama utama padi di Asia sebagai dampak revolusi hijau. Ledakan populasi WBC didukung oleh perilaku WBC yang monofagus pada padi, keperidian tinggi, dan kemampuan migrasi jarak jauh. WBC bersifat labil dalam virulensi dan mampu beradaptasi pada varietas tahan. Penggunaan istilah 'biotipe' untuk pengelompokan virulensi WBC masih diperdebatkan karena adanya variasi virulensi dan genetik antarindividu dalam satu biotipe. Makalah tinjauan ini membahas aspek strategi hidup dan makan, biokimia, interaksi dengan endosimbion, dan genetik WBC untuk mengetahui basis adaptasi virulensi WBC sehingga dapat digunakan dalam perbaikan strategi pengelolaan hama ini. Pada level molekuler, virulensi ditandai dengan peningkatan profil ekspresi gen-gen yang berkaitan dengan detoksifikasi senyawa alelokemik tanaman; metabolisme lipid, karbohidrat, asam amino, dan nitrogen; jalur lintas penyinalan respons pertahanan, respons terhadap cekaman, dan respons kekebalan. Penelitian genomik mengungkap interaksi komplementer antara WBC dan mikroorganisme endosimbion, tetapi percobaan menggunakan WBC aposimbiotik menunjukkan bahwa kepadatan endosimbion tidak selalu berkorelasi positif dengan virulensi WBC. Bertolak belakang dengan hasil-hasil penelitian sebelumnya, pemetaan genetik gen virulensi menggunakan tetua inbred menunjukk...

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“…Currently some 85 genes for resistance to planthoppers and leafhoppers have been identified from rice and its wild relatives [7,14,17]. These genes continue to form a basis for rice resistance breeding programs in Asia; however, they are subject to planthopper and leafhopper adaptation.…”

Section: Aspects Of Virulence Adaptation In Nilaparvata Lugensmentioning

confidence: 99%

“…These varieties, many of which contained the Bph1 or bph2 genes for resistance to N. lugens, were planted throughout Asia [6]; however, the utility of these genes was often short-lived as adapted planthopper populations emerged soon after the varieties were first adopted by farmers [7,10,11]. In response to planthopper adaptation to Bph1 and bph2 resistance, current breeding programs have sought to release new varieties with resistance based on novel (not widely deployed) genes, including Bph3/Bph32 (from Rathu Heenati or PTB33), bph4 (from Babawee), and Bph14 and/or Bph15 (introgressed from Oryza officinalis Well ex Watt) [12][13][14][15]. Breeders have also sought to increase the strength and durability of resistance by pyramiding genes (introgressing two or more resistance loci into a single rice line) using marker-assisted selection [7,16].…”

Section: Introductionmentioning

confidence: 99%

“…Because breeding programs tend to focus on single genes or single donor varieties [7,[12][13][14], comparative studies with multiple donor varieties using a range of planthopper populations are rare [11,16,22]. Furthermore, breeding programs have focused predominantly on resistance in rice seedlings with little information available on resistance in older plants [7,16].…”

Section: Introductionmentioning

confidence: 99%

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Geographic and Research Center Origins of Rice Resistance to Asian Planthoppers and Leafhoppers: Implications for Rice Breeding and Gene Deployment

et al. 2017

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This study examines aspects of virulence to resistant rice varieties among planthoppers and leafhoppers. Using a series of resistant varieties, brown planthopper, Nilaparvata lugens, virulence was assessed in seedlings and early-tillering plants at seven research centers in South and East Asia. Virulence of the whitebacked planthopper, Sogatella furcifera, in Taiwan and the Philippines was also assessed. Phylogenetic analysis of the varieties using single-nucleotide polymorphisms (SNPs) indicated a clade of highly resistant varieties from South Asia with two further South Asian clades of moderate resistance. Greenhouse bioassays indicated that planthoppers can develop virulence against multiple resistance genes including genes introgressed from wild rice species. Nilaparvata lugens populations from Punjab (India) and the Mekong Delta (Vietnam) were highly virulent to a range of key resistance donors irrespective of variety origin. Sogatella furcifera populations were less virulent to donors than N. lugens; however, several genes for resistance to S. furcifera are now ineffective in East Asia. A clade of International Rice Research Institute (IRRI)-bred varieties and breeding lines, without identified leafhopper-resistance genes, were highly resistant to the green leafhopper, Nephotettix virescens. Routine phenotyping during breeding programs likely maintains high levels of

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Pyramiding and evaluation of the brown planthopper resistance genes Bph14 and Bph15 in hybrid rice

Cited by 75 publications

References 22 publications

BAC and RNA sequencing reveal the brown planthopper resistance gene BPH15 in a recombination cold spot that mediates a unique defense mechanism

BAC and RNA sequencing reveal the brown planthopper resistance gene BPH15 in a recombination cold spot that mediates a unique defense mechanism

Virulensi Wereng Batang Cokelat (Nilaparvata lugens Stål) dan Strategi Pengelolaannya

Geographic and Research Center Origins of Rice Resistance to Asian Planthoppers and Leafhoppers: Implications for Rice Breeding and Gene Deployment

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