PVY^NTN elicits a diverse gene expression response in different potato genotypes in the first 12 h after inoculation

Abstract

Host gene expression changes in the early response to potato virus Y(NTN) interaction were compared in two differently sensitive potato cultivars: the resistant cultivar Santé and the sensitive cultivar Igor. Hybridization of potato TIGR cDNA microarrays allowed us to monitor the expression of approximately 10,000 genes simultaneously at 0.5 and 12 h post-inoculation (hpi). Microarray data, analysed by statistics and data mining, were complemented by subtraction library construction and sequence analysis to validate the findings. The expression profiles of the two cultivars were similar and faint at 0.5 hpi, but they differed substantially at 12 hpi. Although, at 0.5 hpi, cv. Santé responded by the differential expression of a greater number of genes, at 12 hpi the number was higher in cv. Igor. The majority of genes in this cultivar were down-regulated at 12 hpi, indicating a host gene shut-off. Suites of genes that exhibited altered transcript abundance in response to the virus were identified, and included genes involved in the processes of photosynthesis, perception, signalling and defence responses. The expression of the considerable number of genes associated with photosynthesis was surprisingly up-regulated as early as 0.5 hpi and down-regulated at 12 hpi in both cultivars. The expression of genes involved in perception and signalling was increased in the sensitive cultivar at 12 hpi. By contrast, a simultaneous strong defence response at the transcriptional level was evident in the resistant cultivar, as shown by the up-regulation of genes involved in brassinosteroid, polyamine and secondary metabolite biosynthesis, and of genes coding for pathogenesis-related proteins.

Differential Gene Expression in Response to Papaya ringspot virus Infection in Cucumis metuliferus Using cDNA- Amplified Fragment Length Polymorphism Analysis

“…2459-Mock (2184). In addition, a previous study reported that in potato, a greater number of differentially expressed genes are found in susceptible than in resistant cultivars in the later stage of Potato virus Y (PVY) infection [25]. Our results were in agreement with it when the total number of polymorphic fragments in susceptible line Acc.…”

“…Because the survival of the obligate pathogens such as viruses lies on the nutrition provided from host plants, and virus infection makes plants changing gene expression patterns and the content of metabolisms to support virus proliferation. It has been suggested that gene expression of host plants infected with pathogens change predominantly in susceptible plants, especially during the late infection stage [25]–[27]. Indeed, the total number of polymorphic fragments observed in susceptible line Acc.…”

“…Only a few studies have shown the changes in gene expression that occur at different time lines after virus inoculation [25], [28]. Since no obvious symptom ever developed in C. metuliferus resistant line PI 292190 after PRSV-inoculation, the only clue that could confirm the success of virus inoculation was to inoculate with the same inoculums in the susceptible line Acc.…”

Dynamics of Responses in Compatible Potato - Potato virus Y Interaction Are Modulated by Salicylic Acid

“…Primers for PR-1b were designed as described in [14] and were 5′-GTATGAATAATTCCACGTACCATATGTTC-3′ (forward primer) and 5′-GTGGAAACAAGAAGATGCAATACTTAGT-3′ (reverse primer).…”

Review Article: Molecular Mechanisms of Resistance to Potato virus X and Y in Potato

“…Baebler et al (2009) detected differences in expression of hundreds of genes as soon as half an hour after inoculation of potato leaves with PVY NTN . Signalling and defence responses specific to cv.…”