PVA and PCL nanofibers are suitable for tissue covering and regeneration

Abstract

The aim of the study was to evaluate the safety and efficacy of a new therapeutic approach to skin defects resulting from split thickness grafting. Within the study, nanofiber-based dressings fabricated using polyvinyl alcohol (PVA) and poly-ε-caprolactone (PCL) were used, with different mass density. The study was performed in 1 female minipig. Nine defects (approx. 4x4 cm) were made in the superficial skin layer. The tested materials were applied to the squared skin defect and covered by a Jelonet paraffin gauze, sutured in the corners of the defects. The animal was monitored daily during the healing process (21 days). On day 5, 12, and 27, the healing of the wound was evaluated, and a biopsy was performed for further histologic testing. At the end of the study (on day 27 after the procedure), the animal was euthanized, and a standard pathologic evaluation was performed. We can conclude that the nanofiber scaffold which was well tolerated, could be used as a smart skin cover which could be functionalized with another bioactive substances directly on the surgeon table, among potential bioactive substances belong platelet derivatives, antibiotics, etc.

Polyblend Nanofibers to Regenerate Gingival Tissue: A Preliminary In Vitro Study

“…Several studies proposed the use of nanofibers to regenerate (Gunn et al, 2010;Chanda et al, 2018) or deliver substances and compounds (Amler et al, 2014;Ahadian et al, 2020) in different tissues (Wang et al, 2017;Beznoska et al, 2019); however, only few preliminary published articles are available in oral fields (Bonilla-Represa et al, 2020;Mohandesnezhad et al, 2020).…”

“…Thus, numerous are the advantages to design polyblend nanofibers. On one side, polyblending would allow to control physicochemical features such as morphology, wettability, formulation, mechanical strength, (Carter et al, 2016); on the other side, polyblending would allow to stimulate biological activity of cells, favoring cell attachment, proliferation, and differentiation (Beznoska et al, 2019).…”

Nanomaterials in Skin Regeneration and Rejuvenation

“…Currently, nanofibers of electrospun poly-ε-caprolactone (PCL) and polyvinyl alcohol (PVA) have been described as safe, well tolerated and effective. They can be used as a smart skin cover dressing during wound healing process alone or in combination with bioactive molecules [20,108]. Among the different subjects functionalized in nanofibers, platelets have been recently described, to create a device delivering platelet-derived bioactive molecules able to improve melanocyte proliferation.…”

Stromal cell-derived factor loaded co-electrospun hydrophilic/hydrophobic bicomponent membranes for wound protection and healing

“…30 Patches based on PCL and PVA combinations are used for wound healing applications with promising outcomes. 31 Electrospinning is a widely accepted method for the generation of nonwoven highly porous submicron ber-based membranes for diverse applications in the biomedical eld. [32][33][34][35][36] Co-electrospinning (or co-spinning) is a highly promising approach employed to produce membranes composed of bers from multiple polymers, even with completely different physicochemical properties.…”

“… 30 Patches based on PCL and PVA combinations are used for wound healing applications with promising outcomes. 31 …”