Putting precision and elegance in enzyme immobilisation with bio-orthogonal chemistry

Abstract

This review focuses on recent advances in the use of elegant bio-orthogonal chemistry in conjunction with rec-DNA to affect highly precise, cost-effective immobilisation of enzymes directly from cell lysate.

The E factor at 30: a passion for pollution prevention

“…by employing bio-orthogonal chemistry to affect precision covalent bonding at preselected sites in the enzyme. 184 Alternatively, affinity immobilisation makes use of polymers containing surface chelating functionalities, such as iminodiacetic acid, preloaded with metal ions -Ni 2+ , Zn 2+ , Fe 2+ , Co 2+ and Cu 2+that exhibit a strong affinity for the imidazole groups in surface histidine residues of the enzyme. It affords high activity recoveries through non-invasive binding.…”

“…by employing bio-orthogonal chemistry to affect precision covalent bonding at preselected sites in the enzyme. 184…”

Engineering ketoreductases for the enantioselective synthesis of chiral alcohols

“…More examples of bio-orthogonal immobilisation strategies can be found in our recently published review. 47…”

“…More examples of bio-orthogonal immobilisation strategies can be found in our recently published review. 47 Immobilisation on aldehyde agarose relies on the reaction between deprotonated e-amino groups of surface lysines and aldehyde groups on the carrier, under alkaline conditions. For example, KRED1-Pglu (from Pichia glucozyma) and BmGDH (from Bacillus megaterium) were immobilised on aldehyde agarose and used in the asymmetric reduction of ketones under continuous flow conditions in a packed bed (Scheme 4).…”

Immobilization and property of penicillin G acylase on amino functionalized magnetic Ni0.3Mg0.4Zn0.3Fe2O4 nanoparticles prepared via the rapid combustion process

“…The surface of nanoparticles is modified by sodium silicate hydrate, and the PGA is crosslinked with the carrier by glutaraldehyde to prepare stable immobilized PGA. Because silica has the advantages of maintaining core magnetism, chemical stability, biocompatibility, and surface modification, and glutaraldehyde is covalently cross-linked with PGA through the free amino groups of lysine residues ( Liu R. J. et al, 2021 ; Liu C. L. et al, 2021 ; Pei et al, 2022 ), both of them are widely used in covalent crosslinking.…”