Engineering ketoreductases for the enantioselective synthesis of chiral alcohols

Abstract

The use of engineered ketoreductases (KREDS), both as whole microbial cells and isolated enzymes, in the highly enantiospecific reduction of prochiral ketones is reviewed. The homochiral alcohol products are key...

Microbial alcohol dehydrogenases: recent developments and applications in asymmetric synthesis

“…Some recent reviews have updated the application of ketoreductases in asymmetric synthesis. [15][16][17]35,105 Li et al described the use of ketoreductases including engineered enzymes that were reported between 2018 and 2020, in the asymmetric synthesis of pharmaceuticals. 15 Another recent review elaborated on the use of wild type and engineered anti-Prelog ADHs in the synthesis of optically active alcohols.…”

“…17 Recently, Qiao et al reviewed protein engineering and the immobilization of keto reductases in the synthesis of chiral alcohols. 105 Here we shine a spotlight on the past five years of the engineering of microbial dehydrogenases towards the synthesis of chiral precursors of drugs and bioactive molecules with industrial applications.…”

Multidisciplinary approaches for enzyme biocatalysis in pharmaceuticals: protein engineering, computational biology, and nanoarchitectonics

“…In addition, ketoreductases are highly efficient biocatalysts for the asymmetric reduction in chiral synthesis, leading to the production of chiral alcohols with high selectivity and purity. 52 Their role in the pharmaceutical industry significantly contributes to the synthesis of key intermediates and compounds required for drug development and manufacturing. 52…”

“…52 Their role in the pharmaceutical industry significantly contributes to the synthesis of key intermediates and compounds required for drug development and manufacturing. 52…”

“…For a detailed overview of various enzyme biocatalysis applications, please refer to Table 1. 51,52,58,59,61,62,65,66,71–74,77–93…”