Putrescine: Essential factor for in vitro proliferation of Babesia bovis

Rojas-Martínez, Carmen; Rodríguez-Vivas, Róger Iván; Millán, Julio Vicente Figueroa; Acosta-Viana, Karla Y.; Ruiz, Edwin José Gutiérrez; Martínez, Jesús Antonio Álvarez

doi:10.1016/j.exppara.2017.01.010

Cited by 13 publications

(18 citation statements)

References 29 publications

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“…When the combination of medium A-DMEM/F12 with ins-trans-sel (2000, 1100, 1.34 mg/L) and Putrescine (Pu, 0.1012 mg/L) was used, maximal PPE of 17.26 and 14.8% were obtained for B. bovis and B. bigemina , respectively. With this culture medium combination, higher growth rates of parasite proliferation were shown ( 92 , 93 ).…”

Section: Elimination Of Bovine Serum In the Culture Medium And Supplementioning

confidence: 90%

“…These new media containing vital elements for eukaryotic cell lines, had not been used until very recently for the in vitro cultivation of protozoan parasites where the maintenance and proliferation of Babesia spp. was accomplished ( 91 , 92 ).…”

Section: Elimination Of Bovine Serum In the Culture Medium And Supplementioning

confidence: 99%

“…This was the first report of the cultivation of Babesia spp. with a perfusion bioreactor, demonstrating the production of Babesia -infected erythrocytes at high density ( 92 , 93 ).…”

Section: Elimination Of Bovine Serum In the Culture Medium And Supplementioning

confidence: 99%

“…Studies on the improvement of the in vitro culture system of B. bovis and B. bigemina have demonstrated that it is possible to expand from a flask of 72 cm 2 containing a PPE of 4% to a perfusion bioreactor, steadily increasing the maximal PPE up to 30% while harvesting material every 24 h. This has been achieved for both B. bovis and B. bigemina culture systems which implies obtaining a larger number of vaccine doses in a shorter period ( 92 , 113 ).…”

Section: Experience On Live Attenuated Vaccines From In Vitmentioning

confidence: 99%

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An Overview of Current Knowledge on in vitro Babesia Cultivation for Production of Live Attenuated Vaccines for Bovine Babesiosis in Mexico

2020

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The instrumentation of the in vitro culture system has allowed researchers to learn more about the metabolic and growth behavior of Babesia spp. The various applications for in vitro cultivation of Babesia include obtaining attenuated strains for vaccination or pre-munition, the selection of pure lines with different degrees of virulence, studies on biological cloning, ultrastructure, antigen production for diagnostics, drug sensitivity assessments, and different aspects of parasite biology. Although there are different types of vaccines that have been tested against bovine babesiosis, so far, the only procedure that has offered favorable results in terms of protection and safety has been the use of live attenuated vaccines. In countries, such as Australia, Argentina, Brazil, Uruguay and Israel, this type of vaccine has been produced and used. The alternative to live vaccines other than splenectomized calf-derived biological material, has been the in vitro cultivation of Babesia bovis and B. bigemina . The development of in vitro culture of Babesia spp. strains in a defined medium has been the basis for the initiation of a source of parasites and exoantigens for a variety of studies on the biochemistry and immunology of babesiosis. The use of live immunogens from attenuated strains derived from in vitro culture is highlighted, which has been proposed as an alternative to control bovine babesiosis. In several studies performed in Mexico, this type of immunogen applied to susceptible cattle has shown the induction of protection against the experimental heterologous strain challenge with both, Babesia -infected blood and animal exposure to confrontations on tick vector-infested farms. The combination of transfection technologies and the in vitro culture system as integrated methodologies would eventually give rise to the generation of genetically modified live vaccines. However, a greater challenge faced now by researchers is the large-scale cultivation of Babesia parasites for mass production and vaccine distribution.

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Section: Elimination Of Bovine Serum In the Culture Medium And Supplementioning

confidence: 90%

Section: Elimination Of Bovine Serum In the Culture Medium And Supplementioning

confidence: 99%

“…This was the first report of the cultivation of Babesia spp. with a perfusion bioreactor, demonstrating the production of Babesia -infected erythrocytes at high density ( 92 , 93 ).…”

Section: Elimination Of Bovine Serum In the Culture Medium And Supplementioning

confidence: 99%

Section: Experience On Live Attenuated Vaccines From In Vitmentioning

confidence: 99%

See 2 more Smart Citations

An Overview of Current Knowledge on in vitro Babesia Cultivation for Production of Live Attenuated Vaccines for Bovine Babesiosis in Mexico

2020

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“…Live and dead cells were calculated by cell counting through hemocytometer. For that, trypan blue was used as a marker for the differentiation of live and dead cells (Rojas-Martinez et al, 2017). Centrifugation of collected blood samples was performed at 3000 rpm for 6 minutes at 4C to obtain the pellet of cells.…”

Section: In Vitro Cultivationmentioning

confidence: 99%

Optimization of Conditions for Cultivation of Babesia bigemina -infected RBCs

Rauf

Rashid

Akbar

et al. 2021

IJAR

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Background: Babesia bigemina is the cause of bovine babesiosis. B. bigemina-infected red blood cells (iRBCs) were passaged in vitro for the attenuation. Methods: We cultured Pakistani isolate of the parasite in three different culture media. Whole blood was collected from splenectomized and intact crossbred young calves. The parameters included were hematological profile, catalase activity, osmotic fragility and lipid profile. Cell culture media (M-199, RPMI 1640 and DMEM) were used to find out the longevity of parasites iRBCs.Result: Highest PPE level was found up to 6.0% on 72 h post-culture in M-199 medium. Furthermore, no significant difference in catalase activity while significant difference in osmotic fragility were observed. However, lipid profile was significantly less in infected animals except in Babesia-infected. M-199 was the most appropriate medium for the in vitro cultivation of B. bigemina. Our findings would help us for in vitro cultivation of babesia-infected RBCs for its attenuation.

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The role of the complement factor B‒arginase‒polyamine molecular axis in uremia‐induced cardiac remodeling in mice

Yang

Song

et al. 2019

Eur J Immunol

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The role of complement system in heart diseases is controversial. Besides, the mechanisms by which complement components participate in cardiac remodeling (CR) and heart failure during uremia are unclear. In this study, 5/6 nephrectomy was performed to adult mice to establish the uremic model and CR deteriorated over the course of uremia. Although complement pathways were not further activated over the course of the disease, soluble complement factor B (CFB) was upregulated at post-nephrectomy day 90 (PNx90) compared with PNx30. Further, CFB notably deteriorated CR in uremic mice but this effect was reversed by depletion of macrophages with liposomal clodronate. In vivo and in vitro CFB upregulated arginase 1 (ARG1) expression, increased ARG1 enzymatic activity, and stimulated the syntheses of ornithine, leading to polyamine overproduction in macrophages. Putrescine, an important polyamine, promoted cardiac fibroblast proliferation and collagen production, resulting in progressive CR. In vivo the inhibition of ARG1 activity with N ω -hydroxyl-L-arginine remarkably improved the general survival rates, inhibited the infiltration of cardiac fibroblasts, and alleviated progression of CR in uremic mice. Taken together, the CFB-ARG1-putrescine axis is related to progression of CR and ARG1 hyperactivity in macrophages may provide a novel therapeutic target against the heart injury in uremia.Keywords: complement r heart failure r macrophage r polyamine r uremia Additional supporting information may be found online in the Supporting Information section at the end of the article.

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Putrescine: Essential factor for in vitro proliferation of Babesia bovis

Cited by 13 publications

References 29 publications

An Overview of Current Knowledge on in vitro Babesia Cultivation for Production of Live Attenuated Vaccines for Bovine Babesiosis in Mexico

An Overview of Current Knowledge on in vitro Babesia Cultivation for Production of Live Attenuated Vaccines for Bovine Babesiosis in Mexico

Optimization of Conditions for Cultivation of Babesia bigemina -infected RBCs

The role of the complement factor B‒arginase‒polyamine molecular axis in uremia‐induced cardiac remodeling in mice

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