Putative nitric oxide synthase containing nervous elements in male and female gonads of some marine bivalve mollusks revealed by NADPH-diaphorase histochemistry

Анникова, Л В; Dyuizen, I. V.; Paltseva, Y. N.; Вараксин, А. А.

doi:10.1080/07924259.2001.9652499

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…The staining intensity of nerve fiber varicosities, where NO is presumably actively synthesized, is especially high. NADPH-d-positive peripheral neurons and nerve fibers are found in various parts of the intestinal tract, as well as in female and male gonads in a number of bivalve mollusk species (Annikova et al, 2001;Pimenova et al, 2002;Varaksin et al, 2002;Pimenova and Varaksin, 2006). These findings suggest that NO is involved in regulation of reproduction and digestion in bivalve mollusks.…”

Section: Discussionmentioning

confidence: 82%

NADPH-diaphorase activity in the central nervous system of the Gray mussel Crenomytilus grayanus (Dunker) under stress conditions: A histochemical study

Vaschenko¹,

Kotsyuba²

2008

Marine Environmental Research

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NADPH-diaphorase (NADPH-d) is a histochemical marker for nitric oxide synthase (NOS) and is widely used to identify nitric oxide (NO) producing cells in the central nervous system (CNS) of both vertebrates and invertebrates. NADPH-d histochemistry was used to quantitatively characterize putative NO-producing neurons in the CNS of the Gray mussel Crenomytilus grayanus subjected to two kinds of stress, environmental pollution and hypoxia, the latter caused by the mollusk transportation in a small volume of water. Mussels were sampled from one relatively clean (reference) and four polluted sites in Amursky and Ussuriysky Bays (Peter the Great Bay, Sea of Japan) in August, 2003. The number of NADPH-d-positive neurons was estimated and enzyme activity was determined from the optical density of the formazan precipitate in the CNS ganglia at 0, 3, and 72 h after sampling. Just after sampling, NADPH-d-positive neurons were found in the cerebropleural, visceral, and pedal ganglia. The number and staining intensity of NADPH-d-positive neurons were significantly higher in the pedal ganglia than the other two ganglia. There were significant differences in the number of NADPH-d-positive neurons and enzyme activity between the mussels from the reference and heavily polluted stations. The proportion and staining intensity of NADPH-d-positive neurons were maximum in the pedal ganglia of the mussels from the heavily polluted station in Amursky Bay. Transportation of mussels in a limited volume of water for 3h resulted in a significant increase in the proportion and staining intensity of NADPH-d-positive neurons in all ganglia. In mollusks from all stations kept in aerated aquaria for 72 h, both the proportion and staining intensity of NADPH-d-positive neurons did not differ significantly from the initial level. However, the differences in the proportion and staining intensity of NADPH-d-positive neurons between the reference and heavily polluted stations were significant. The present results suggest that NO is involved in mollusk nerve cell adaptation to environmental changes.

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Section: Discussionmentioning

confidence: 82%

NADPH-diaphorase activity in the central nervous system of the Gray mussel Crenomytilus grayanus (Dunker) under stress conditions: A histochemical study

Vaschenko¹,

Kotsyuba²

2008

Marine Environmental Research

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“…VIP and nNOS immunoreactivity elevated at the periphery of the cytoplasm of the oocytes and in the follicular cells, and the evidence of network of nerve fibres between oocytes during vitellogenesis, as found in other species [8,10] suggested that the presence of NO and its synergism with VIP is a challenging subject to study due to wide variety of biological actions of NO and VIP on regulation of gametogenesis and spawning of both invertebrates and vertebrates.…”

Section: Discussionmentioning

confidence: 99%

“…The phylogeny and functional roles of nNOS and VIP, present in different tissues (epidermic, tegumental connective, musculature of swim-bladder, gills, lung) of Invertebrates and Vertebrates are still unclear. However recent studies indicate involvement of these bioactive compounds in the non adrenergic-non cholinergic (NANC) regulatory mechanism [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus (V).

Calabrò¹,

Albanese²,

Bertuccio³

et al. 2008

Folia Histochem Cytobiol.

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Abstract:The oocytes of the abyssal Teleost, Hoplostethus mediterraneus were studied. Four stages of growth were observed and the oocytes of all the stages were surrounded by follicular cells and had several nucleoli in the nucleus. In the oocytes of the II° stage, vacuoles without contents, in oocytes of the III° stage several vacuoles with a basophilic contents and small yolk globules were identified. General and basic proteins, ribonucleoproteins, acid proteoglycans with -COOH groups were recognized in the cytoplasm, in the nucleoli of oocytes in the II° stage and in the vacuolar contents of oocytes in the III° stage. In the follicular cells, in the pellucid zone, in the yolk globules, from their beginning, glycoproteins were present. Positivity, for all lectins used, was revealed in the follicular cells and in the four stages of oocytes growth. α-D-glucose and α-D-mannose binding sites were in the pellucid zone and in the initial yolk globules. In the lather galactose and β-N-acetyl glucosamine were present too. nNOS and VIP immunopositivity revealed at the periphery of the cytoplasm and at network of nerve fibres between oocytes, suggests NO is involved in a mechanism of regulation of the gametogenesis and of the spawning.

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Immunohistochemical localization of nNOS and VIP in the mantle integument of the mussel, Mytilus galloprovincialis

et al. 2003

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Putative nitric oxide synthase containing nervous elements in male and female gonads of some marine bivalve mollusks revealed by NADPH-diaphorase histochemistry

Cited by 4 publications

References 19 publications

NADPH-diaphorase activity in the central nervous system of the Gray mussel Crenomytilus grayanus (Dunker) under stress conditions: A histochemical study

NADPH-diaphorase activity in the central nervous system of the Gray mussel Crenomytilus grayanus (Dunker) under stress conditions: A histochemical study

Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus (V).

Immunohistochemical localization of nNOS and VIP in the mantle integument of the mussel, Mytilus galloprovincialis

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