Immunohistochemical localization of nNOS and VIP in the mantle integument of the mussel, Mytilus galloprovincialis

Licata, A.; Ainis, L.; Martella, Silvestro; Ricca, M. B.; Licata, Patrizia; Pergolizzi, Simona; Calabrò, Concetta; Zaccone, Giacomo

doi:10.1078/0065-1281-00700

Cited by 10 publications

(5 citation statements)

References 31 publications

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“…VIP and nNOS immunoreactivity elevated at the periphery of the cytoplasm of the oocytes and in the follicular cells, and the evidence of network of nerve fibres between oocytes during vitellogenesis, as found in other species [8,10] suggested that the presence of NO and its synergism with VIP is a challenging subject to study due to wide variety of biological actions of NO and VIP on regulation of gametogenesis and spawning of both invertebrates and vertebrates.…”

Section: Discussionmentioning

confidence: 99%

“…The phylogeny and functional roles of nNOS and VIP, present in different tissues (epidermic, tegumental connective, musculature of swim-bladder, gills, lung) of Invertebrates and Vertebrates are still unclear. However recent studies indicate involvement of these bioactive compounds in the non adrenergic-non cholinergic (NANC) regulatory mechanism [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…The aim of this study was the investigation on the oocytes of abyssal teleost Hoplostethus mediterraneus in order to ascertain the presence of bioactive molecules too [9][10][11][12][13] and to confirm their role in the reproductive function.…”

Section: Introductionmentioning

confidence: 99%

“…A recent immunohistochemical investigation on the mantle integument of the mussel Mytilus galloprovincialis [10], in order to detect, in both sexes, the presence of nNOS and VIP, resulted positive in cortex of sperm follicles and nNOS positive sperm only in the male, in the female positivity in the nerve cells bodies and fibres and negativity in the maturating oocytes.…”

Section: Introductionmentioning

confidence: 99%

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Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus (V).

Calabrò¹,

Albanese²,

Bertuccio³

et al. 2008

Folia Histochem Cytobiol.

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Abstract:The oocytes of the abyssal Teleost, Hoplostethus mediterraneus were studied. Four stages of growth were observed and the oocytes of all the stages were surrounded by follicular cells and had several nucleoli in the nucleus. In the oocytes of the II° stage, vacuoles without contents, in oocytes of the III° stage several vacuoles with a basophilic contents and small yolk globules were identified. General and basic proteins, ribonucleoproteins, acid proteoglycans with -COOH groups were recognized in the cytoplasm, in the nucleoli of oocytes in the II° stage and in the vacuolar contents of oocytes in the III° stage. In the follicular cells, in the pellucid zone, in the yolk globules, from their beginning, glycoproteins were present. Positivity, for all lectins used, was revealed in the follicular cells and in the four stages of oocytes growth. α-D-glucose and α-D-mannose binding sites were in the pellucid zone and in the initial yolk globules. In the lather galactose and β-N-acetyl glucosamine were present too. nNOS and VIP immunopositivity revealed at the periphery of the cytoplasm and at network of nerve fibres between oocytes, suggests NO is involved in a mechanism of regulation of the gametogenesis and of the spawning.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus (V).

Calabrò¹,

Albanese²,

Bertuccio³

et al. 2008

Folia Histochem Cytobiol.

Self Cite

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“…In annelids, a sister clade of molluscs, immunoreactive PACAP-like peptides and receptors were identified in the CNS and peripheral nervous system (PNS) (Reglödi et al, 2000;Molnar et al, 2006;Boros et al, 2008Boros et al, , 2010Varhalmi et al, 2008). Similarly, in the bivalve mollusc the Mediterranean mussel (Mytilus galloprovincialis), immunoreactivity for VIP was detected in the mantle and in gastropod molluscs, VIP-like molecules were detected in the nervous system of the sea hare (Aplysia kurodai) and land snail (Helix pomatia) and in innate immune cells of two freshwater snails (Planorbarius corneus and Viviparus ater) (Kuramoto et al, 1985;Ottavianil and Cossarizza, 1990;Ottaviani et al, 1992;Kaufmann et al, 1995;Licata et al, 2003). In the nervous system of the pond snail (Lymnaea stagnalis) two VIP immunoreactive neurons were detected (Schot et al, 1981).…”

Section: Lophotrochozoansmentioning

confidence: 99%

Tracing the Origins of the Pituitary Adenylate-Cyclase Activating Polypeptide (PACAP)

2020

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Pituitary adenylate cyclase activating polypeptide (PACAP) is a well-conserved neuropeptide characteristic of vertebrates. This pluripotent hypothalamic neuropeptide regulates neurotransmitter release, intestinal motility, metabolism, cell division/differentiation, and immunity. In vertebrates, PACAP has a specific receptor (PAC 1) but it can also activate the Vasoactive Intestinal Peptide receptors (VPAC 1 and VPAC 2). The evolution of the vertebrate PACAP ligand-receptor pair has been well-described. In contrast, the situation in invertebrates is much less clear. The PACAP ligand-receptor pair in invertebrates has mainly been studied using heterologous antibodies raised against mammalian peptides. A few partial PACAP cDNA clones sharing >87% aa identity with vertebrate PACAP have been isolated from a cnidarian, several protostomes and tunicates but no gene has been reported. Moreover, current evolutionary models of the peptide and receptors using molecular data from phylogenetically distinct invertebrate species (mostly nematodes and arthropods) suggests the PACAP ligand and receptors are exclusive to vertebrate genomes. A basal deuterostome, the cephalochordate amphioxus (Branchiostoma floridae), is the only invertebrate in which elements of a PACAP-like system exists but the peptides and receptor share relatively low sequence conservation with the vertebrate homolog system and are a hybrid with the vertebrate glucagon system. In this study, the evolution of the PACAP system is revisited taking advantage of the burgeoning sequence data (genome and transcriptomes) available for invertebrates to uncover clues about when it first appeared. The results suggest that elements of the PACAP system are absent from protozoans, non-bilaterians, and protostomes and they only emerged after the protostome-deuterostome divergence. PACAP and its receptors appeared in vertebrate genomes and they probably shared a common ancestral origin with the cephalochordate PACAP/GCG-like system which after the genome tetraploidization events that preceded the vertebrate radiation generated the PACAP ligand and receptor pair and also the other members of the Secretin family peptides and their receptors.

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First report of the pituitary adenylate cyclase activating polypeptide (PACAP) in crustaceans: Conservation of its functions as growth promoting factor and immunomodulator in the white shrimp Litopenaeus vannamei

Lugo

Carpio

Morales

et al. 2013

Fish & Shellfish Immunology

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Immunohistochemical localization of nNOS and VIP in the mantle integument of the mussel, Mytilus galloprovincialis

Cited by 10 publications

References 31 publications

Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus (V).

Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus (V).

Tracing the Origins of the Pituitary Adenylate-Cyclase Activating Polypeptide (PACAP)

First report of the pituitary adenylate cyclase activating polypeptide (PACAP) in crustaceans: Conservation of its functions as growth promoting factor and immunomodulator in the white shrimp Litopenaeus vannamei

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