Purinergic Regulation of Basic Physiological and Pathological Processes

Сергеевич, Головкин Алексей; Асадуллина, И А; Кудрявцев, И. В.

doi:10.15789/1563-0625-2018-4-463-476

Cited by 5 publications

(4 citation statements)

References 99 publications

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“…Previously, it was demonstrated that purinergic signaling played an important role in regulating T-cell functions (Ledderose et al, 2016; Golovkin et al, 2018). T cells migrating into the damaged heart tissues upregulate enzymatic machinery for degrading extracellular ATP and NAD to adenosine (Borg et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…A significant number of studies have been recently published evidencing about an important role played by the receptors for extracellular ATP and its metabolites as well as enzymes involved in regulating their metabolism in vascular and heart valve calcification (Fish et al, 2013; Towler, 2017; Dou et al, 2018; Golovkin et al, 2018; Kaniewska-Bednarczuk et al, 2018). Previously, it was shown that various receptor isotypes specific to extracellular adenosine (P1 family) and ATP (Р2Х and Р2Y families) were found on smooth muscle cells, the major cell type involved in formation of calcium depositions in the vascular wall (Fish et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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CD73 Rather Than CD39 Is Mainly Involved in Controlling Purinergic Signaling in Calcified Aortic Valve Disease

et al. 2019

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The study aimed to compare composition of peripheral blood T-cell subsets and assess their surface expression of CD39 and CD73 ectonucleotidases in patients with severe and moderate aortic stenosis (AS) as well as to evaluate involvement of T-cell-mediated immune processes in valve calcification. The study was performed with 38 patients suffering from severe calcified aortic stenosis (SAS), 33 patients with MAS, and 30 apparently healthy volunteers (HVs). The relative distribution and percentage of T-cell subsets expressing CD39 and CD73 were evaluated by flow cytometry. T helper (Th) and cytotoxic T-cell subsets (Tcyt) were identified by using CD3, CD4, and CD8 antibodies. Regulatory T cells (Tregs) were characterized by the expression of CD3, CD4, and high IL-2R alpha chain (CD25high) levels. CD45R0 and CD62L were used to assess differentiation stage of Th, Tcyt, and Treg subsets. It was found that MAS and SAS patients differed in terms of relative distribution of Tcyt and absolute number of Treg. Moreover, the absolute number of Tcyt and terminally differentiated CD45RA-positive effector T-cells (TEMRA) subset was significantly higher in SAS vs. MAS patients and HVs. However, the absolute and relative number of naïve Th and the absolute number of Treg were significantly higher in MAS vs. SAS patients; the relative number of naïve Tregs was significantly ( p < 0.01) decreased in SAS patients. It was shown that CD73 expression was significantly higher in SAS vs. MAS patients noted in all EM, CM, TEMRA, and naïve Th cell subsets. However, only the latter were significantly increased ( p = 0.003) in patients compared with HVs. SAS vs. MAS patients were noted to have significantly higher percentage of CD73+ EM Tcyt ( p = 0.006) and CD73+ CM Tcyt ( p = 0.002). The expression of CD73 in patients significantly differed in all three Treg populations such as EM ( p = 0.049), CM ( p = 0.044), and naïve ( p < 0.001). No significant differences in CD39 expression level was found in MAS and SAS patients compared with the HV group. Overall, the data obtained demonstrated that purinergic signaling was involved in the pathogenesis of aortic stenosis and calcification potentially acting via various cell types, wherein among enzymes, degrading extracellular ATP CD73 rather than CD39 played a prominent role.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

CD73 Rather Than CD39 Is Mainly Involved in Controlling Purinergic Signaling in Calcified Aortic Valve Disease

et al. 2019

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“…Растущие и регенерирующие ткани, а также ткани с высоким уровнем клеточного оборота характеризуются продукцией различных ростовых и трансформирующих рост факторов, аденозина, адреномедуллина, проадреномедуллина, метаболических цитокинов (например, FGF-21) и других медиаторов, препятствующих развитию инсулинорезистентности (в факультативно гликолизирующих тканях) и выраженной провоспалительной активности клеток [1,62,83,146]. По мере прогрессирования тканевого стресса продукция этих медиаторов может возрастать, но уже на фоне доминирующей роли провоспалительных цитокинов [83,229].…”

Section: Sr в обеспечении пролиферации клеток и тканевого ростаunclassified

Physiological and pathogenic role of scavenger receptors in humans

et al. 2020

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The scavenger receptors (SRs)) include > 30 different molecules structurally classified into 11 classes (A to L). They are expressed mostly on stromal macrophages, and their expression may be augmented in direct dependence with concentrations of their ligands. The SRs are heterogenous by their structure, however, being common in their functional potential. E.g., different SR classes may participate in absorption of modified low-density lipoproteins and glycated proteins, apoptotic and ageing cells, altered erythrocytes and platelets, like as a big variety of other endogenous ligands from metabolic and cellular “trash”. A common property of SRs is their participation in removal of small pathogen amounts from blood circulation, regulation of cell and tissue stress responses, ability to form complicated receptor complexes with other receptor types including integrins and toll-like receptors. Opposite to classic pattern-recognizing receptors, the SR involvement does not always elicit a pronounced cellular activation and development of pro-inflammatory cellular stress. The SR functional effects provide interactions between different physiological events and immune system, including the processes of neuroendocrine and metabolic regulation. These mechanisms provide both homeostatic stability and, likewise, act at the border of normal and pathological conditions, i.e., participating in pathogenesis of transitional processes, e.g., physiological ageing. Moreover, the SR-associated processes represent a key pathogenetic factor in different somatic diseases, e.g., those associated with low-intensity chronic inflammation, including obesity, type 2 diabetes, atherosclerosis, arterial hypertension, various neurodegenerative disorders. Similarly, the SRs are involved into the processes of cancer transformation and antitumor response, different processes of classical inflammation, from antigen presentation to the morphofunctional T cell and macrophage polarization in the inflammation foci and immunocompetent organs. SR are playing a controversial role in development of acute systemic inflammation, the main reason for lethal outcomes in the intensive care wards. Targeted effects upon the SRs represent a promising approach when treating a broad variety of diseases, whereas detection of membrane-bound and soluble SR forms could be performed by means of diagnostic and monitoring techniques in many human disorders.

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“…One of the most important mechanisms of suppression of inflammation, which is realized by Treg both in lymphoid tissue and in the site of inflammation, is the degradation of pro-inflammatory extracellular ATP to adenosine, which has a wide spectrum of antiinflammatory action and acts on the effector cells of innate and acquired immunity [8]. When comparing CD39 + and CD39 -Treg in vitro, it was shown that the presence of CD39 is closely related to the high ability of these cells to show their suppressor properties due to the efficient expression of the transcription factor FoxP3, as well as the regulatory molecules CTLA4, GARP, and LAP [28].…”

Section: Expression Of Cd39 and Cd73 By Various Subpopulations Of Regmentioning

confidence: 99%

Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome

Ermolenko¹,

Кудрявцев

Соловьева

et al. 2020

Med. immunol.

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Резюме. При помощи метагеномного анализа (16 S rRNA) выявлены особенности соcтава кишечного микробиоценоза у больных синдромом раздраженного кишечника (СРК): 1) увеличение представительства Actinobacteria, в том числе Bifidobacterium spp., Firmicutes, в том числе принадлежащих к семействам Streptococcaceae (Streptococcus), Lachnosperaceae (Dorea), Veillonellaceae (Dialister), Proteobacteria (семейства Enterobacteriaceae и Desulfovibrionaceae); 2) уменьшение популяции Bacteroidetes, в том числе представителей семейств Prevotellacea (Prevotella spp.), Bacteroidaceae (Bacteroides spp.), фирмикутных бактерий, относящихся к семействам Clostridiaceae и Ruminococcaceae (Fecalibacterium spp.). Проточная цитометрия при исследовании субпопуляционного состава T-регуляторных (Тreg) лимфоцитов выявила у больных СРК увеличение количества CD45R0 + CD62L + клеток центральной памяти (СМ), способных регулировать процессы созревания и дифференцировки лимфоцитов в лимфоидной ткани. Обнаружено снижение экспрессии экзонуклеаз CD39 и CD73, что может оказывать существенное влияние на их активность. Отмечено снижение эффекторных клеток памяти (ЕМ) Тreg. Изменения в уровне экспрессии экзонуклеаз CD39 и CD73 находились в обратной корреляционной связи с содержанием протеобактерий и представительством родов Bifidobactrium и Faecalibacterium. Количественное содержание СМ Treg находилось в прямой корреляционной связи с содержанием Dorea spp. Полученные результаты могут указывать на нарушения в процессах дифференцировки Тreg, которые тесно связаны с изменениями ключевых компонентов кишечного микробиоценоза при СРК.

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Purinergic Regulation of Basic Physiological and Pathological Processes

Cited by 5 publications

References 99 publications

CD73 Rather Than CD39 Is Mainly Involved in Controlling Purinergic Signaling in Calcified Aortic Valve Disease

CD73 Rather Than CD39 Is Mainly Involved in Controlling Purinergic Signaling in Calcified Aortic Valve Disease

Physiological and pathogenic role of scavenger receptors in humans

Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome

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