Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome

Ermolenko, E. I.; Кудрявцев, И. В.; Соловьева, О. И.; Karaseva, Alena; Tsapieva, Anna N.; Кондратенко, Ю. Д.; Kotyleva, M. P.; Серебрякова, М. К.; Шумихина, И. А.; Suvorov, Alexander

doi:10.15789/1563-0625-pos-1905

Cited by 3 publications

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References 36 publications

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“…Принципиальным различием явилось обнаружение у жителей Ханоя при СРК большего содержания по сравнению с больными после терапии и здоровыми людьми лактобацилл, относящихся к фирмикутам. Очевидно, это связано с особенностью микробиоты жителей азиатского региона, выявленной ранее [9,23].…”

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Irritable bowel syndrome therapy in Vietnam with probiotic enterococci

Ermolenko

Kotyleva

Tsapieva

et al. 2022

jour

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In this work, we compared composition of the intestinal microbiota of healthy volunteers and patients with irritable bowel syndrome (IBS) in Hanoi residents before and after therapy with a probiotic starter culture based on the bacteriocinogenic strain Enterococcus faecium L3 (L3), which is successfully used to correct dysbiosis and treat IBS in Russia.In IBS patients (IBS group) α-diversity was higher than in the control group (Healthy) and in IBS patients after probiotic therapy (IBS + L3 group). Phylogenetic analysis of the studied samples refl ected the division of the studied communities into 2 clusters, one of which grouped samples, mainly taken for IBS before therapy. Principal component analysis (PCoA) showed that most of the samples from the IBS group are located on the graph in a separate sector, far from the adjacent IBS + L3 and Нealthy samples.A study of the microbiome of Vietnamese patients with IBS after taking probiotic at the level of types (phyls) revealed a tendency towards an increase in the representation of Firmicutes. The introduction of L3 led to an increase in the population of members of the family Lachnospiraceae, such as butyrate- producing Roseburia and acetate-producing Blautia. At the same time, the percentage of staphylococci and bacteria belonging to the Enterobacteriaceae family was decreased. In addition to positive changes in the functioning of the gastrointestinal tract, revealed on the basis of clinical data, the features of the intestinal microbiota of IBS residents of Hanoi compared with healthy volunteers and in the same patients after probiotic therapy were revealed by metagenomic analysis of 16S rRNA genes. Positive shifts in the intestinal microbiome of IBS patients in Vietnam after therapy with a probiotic starter culture based on L3 do not fundamentally diff er from the previously described eff ects noted by the authors when using this probiotic in Russia, which creates the preconditions for expanding the possibility of its use in the Asian region.

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“…), фирмикутных бактерий, относящихся к семействам Clostridiaceae и Ruminococcaceae (Faecalibacterium spp.) [9].…”

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Irritable bowel syndrome therapy in Vietnam with probiotic enterococci

Ermolenko

Kotyleva

Tsapieva

et al. 2022

jour

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Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome

Ermolenko¹,

Кудрявцев

Соловьева

et al. 2020

Med. immunol.

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Резюме. При помощи метагеномного анализа (16 S rRNA) выявлены особенности соcтава кишечного микробиоценоза у больных синдромом раздраженного кишечника (СРК): 1) увеличение представительства Actinobacteria, в том числе Bifidobacterium spp., Firmicutes, в том числе принадлежащих к семействам Streptococcaceae (Streptococcus), Lachnosperaceae (Dorea), Veillonellaceae (Dialister), Proteobacteria (семейства Enterobacteriaceae и Desulfovibrionaceae); 2) уменьшение популяции Bacteroidetes, в том числе представителей семейств Prevotellacea (Prevotella spp.), Bacteroidaceae (Bacteroides spp.), фирмикутных бактерий, относящихся к семействам Clostridiaceae и Ruminococcaceae (Fecalibacterium spp.). Проточная цитометрия при исследовании субпопуляционного состава T-регуляторных (Тreg) лимфоцитов выявила у больных СРК увеличение количества CD45R0 + CD62L + клеток центральной памяти (СМ), способных регулировать процессы созревания и дифференцировки лимфоцитов в лимфоидной ткани. Обнаружено снижение экспрессии экзонуклеаз CD39 и CD73, что может оказывать существенное влияние на их активность. Отмечено снижение эффекторных клеток памяти (ЕМ) Тreg. Изменения в уровне экспрессии экзонуклеаз CD39 и CD73 находились в обратной корреляционной связи с содержанием протеобактерий и представительством родов Bifidobactrium и Faecalibacterium. Количественное содержание СМ Treg находилось в прямой корреляционной связи с содержанием Dorea spp. Полученные результаты могут указывать на нарушения в процессах дифференцировки Тreg, которые тесно связаны с изменениями ключевых компонентов кишечного микробиоценоза при СРК.

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Use of autoprobiotics in the complex therapy of axial spondyloarthritis

Artemev

Ermolenko

Kotyleva

et al. 2023

jour

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Spondyloarthritis (SpA) is a group of chronic inflammatory diseases of the musculoskeletal system involving of the axial skeleton and extra-articular manifestations such as inflammatory bowel diseases. Some violations of the intestinal microbiome often occur during the course of spondyloarthritis. Also, intestinal dysbiosis can be enhanced by ongoing therapy. The aim of the study was to evaluate the effectiveness of combined therapy with nonsteroidal anti-inflammatory drugs (NSAIDs) and autoprobiotics supplementation.SpA patients treated with NSAID were divided into two groups: group A which took autoprobiotic based on indigenous culture of Enterococcus faecium, and group S which took only Supra medium, which is the basis used for making of autoprobiotic. Reducing of pain intensity, dyspeptic phenomena were observed to a greater extent in group A compared to group S. PCRRT testing revealed no significant changes in intestinal microbiocenosis in patients with SpA, except of a decrease in the Lactobacillus population, which was restored only in group A. A feature of the changes in group S was a decrease in the total bacterial mass, amounts of Bacteroides, Faecalibacterium, Enterobacter and expansion of Methanobrevibacter population. The tendency to restore the quantitative content of Lactobacillus, correlating with a decrease of IL-10 concentration, was found only in group A.In our study the effectiveness of enterococcal auprobiotic supplementation as an element of complex therapy of patient suffering from SpA has been proven. The use of an autoprobiotic leads to a decrease in the severity of the symptoms of the disease, the leveling of dyspeptic symptoms and microbiota disorders.

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Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome

Cited by 3 publications

References 36 publications

Irritable bowel syndrome therapy in Vietnam with probiotic enterococci

Irritable bowel syndrome therapy in Vietnam with probiotic enterococci

Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome

Use of autoprobiotics in the complex therapy of axial spondyloarthritis

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