Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum

Wang, Yongjie; Liu, Na; Xue, Xindong; Li, Qiang; Sun, Deqing; Zhao, Zhongxi

doi:10.1016/j.ijbiomac.2020.05.245

Cited by 91 publications

(59 citation statements)

References 38 publications

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“…After 48 h incubation, the order of effect of samples on phagocytic activity at their optimal concentration was as follows: ESFP2>ESFP1>ESFP3>ESFP4 ( Figure 6 d ). The influence of the polysaccharide on the phagocytosis activity of macrophages may be related to the binding of the polysaccharide with a specific receptor on the surface of the macrophages [24] . The difference in phagocytosis of the four fractions may result from their structures, e. g., molecular weight, monosaccharide components [24,25] .…”

Section: Resultsmentioning

confidence: 99%

“…The influence of the polysaccharide on the phagocytosis activity of macrophages may be related to the binding of the polysaccharide with a specific receptor on the surface of the macrophages [24] . The difference in phagocytosis of the four fractions may result from their structures, e. g., molecular weight, monosaccharide components [24,25] . The phagocytic activity of RAW264.7 macrophages increased when compared to that in the case of 24 h incubation, whereas positive control LPS displayed better activity than the four fractions after 48 h incubation ( P< 0.05).…”

Section: Resultsmentioning

confidence: 99%

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Purification and Biological Activities of Enzymatically Degraded Sargassum fusiforme Polysaccharides

Jiang

Yang

Chakka

et al. 2021

Chemistry & Biodiversity

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Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme was purified by column DEAE‐52 and Sephadex G‐100 to yield four components, namely, ESFP1, ESFP2, ESFP3 and ESFP4. These components were characterized by chemical composition assay, GC/MS, HPGPC, UV and FT‐IR techniques. The in vitro antioxidant activities of the four purified fractions were investigated by measuring their radical scavenging activity and reducing power. The results suggested that all the four components possess good antioxidant activities. Among them, ESFP1 was found to possess the strongest 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical and hydroxyl radical‐scavenging activity, and the greatest ferric reducing power. The immunomodulatory effect of these four polysaccharides was demonstrated by their ability to promote proliferation, and to enhance both phagocytic activity and NO release in a macrophage RAW264.7 model. The results revealed that the bioactivities of the polysaccharides are related to their molecular weight, and the uronic acid and sulfate contents.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Purification and Biological Activities of Enzymatically Degraded Sargassum fusiforme Polysaccharides

Jiang

Yang

Chakka

et al. 2021

Chemistry & Biodiversity

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“…In the 1 H NMR spectra, the signal at 5.35 ppm was designated as α‐type glycosidic bonds in PCP (Wang et al ., 2015). The signal at δ 2.09 ppm was attributed to the O‐acetyl methyl protons, indicating an acetyl substitution reaction in a different glycosidic residue position (Wang et al ., 2020). The signal at high field (δ 1.12 ppm) was assigned to the methyl group protons, whereas the CH 3 group was likely derived from rhamnose (C–6) (Guo et al ., 2015).…”

Section: Resultsmentioning

confidence: 99%

“…The small signal at 93.15 ppm was attributed to C–1, an internal α‐D‐glucopyranose (Glcp) (Wang et al ., 2019). Based on the PCP monosaccharide composition and literature, the signals at 103.20–104.06 ppm and 61.02–82.19 ppm were assigned to the β‐anomeric configuration of Galp, Glcp, Rha, and Manp (Yu et al ., 2017; Wang et al ., 2018b; Dammak et al ., 2019; Wang et al ., 2020). These findings concur with those obtained from the FT‐IR spectrum analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Among them, polysaccharides are believed to be significant bioactive components (Cui et al ., 2018). The molecular weight, monosaccharide composition and polysaccharides structure of different Polygonatum varieties are diverse, leading to the difference in their functioning (Liu et al ., 2017; Zhao et al ., 2019; Wang et al., 2020a,2020b). Gu et al .…”

Section: Introductionmentioning

confidence: 99%

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Structural analysis and anti‐obesity effect of Polygonatum cyrtonema polysaccharide against obesity induced by high‐fat diet in mice

Liang

Kai³

et al. 2021

Int J of Food Sci Tech

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This study aimed to analyse the physicochemical properties of Polygonatum cyrtonema polysaccharide (PCP) and evaluated its effects in a high‐fat diet (HFD)‐induced obese mice. Experimentally, PCP was established as a homogeneous heteropolysaccharide with both α‐ and β‐configurations, and its estimated average molecular weight was ˜5.1 kDa. Besides, we discovered its major monosaccharides, including galactose, mannose, rhamnose, galacturonic acid and glucose. PCP markedly decreased obesity‐related parameters, improved serum lipid profile, fasting blood glucose, and leptin levels and reversed insulin resistance. It also reduced the hepatic infiltration of lipid droplets and the size of adipocytes in adipose tissues. Finally, PCP simulation significantly downregulated the mRNA expression of CCAAT/enhancer‐binding protein‐α (C/EBPα), peroxisome proliferator‐activated receptor‐γ (PPARγ), sterol regulatory element‐binding protein‐1c (SREBP‐1c) and fatty acid synthase (FAS). On the contrary, it increased carnitine palmitoyltransferase (CPT1) and uncoupling protein 2 (UCP2) levels closely related to thermogenesis and glucolipid metabolism. Taken together, these findings have provided evidence that PCP could be a potential anti‐obesity ingredient in preventing diet‐induced obesity.

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Structure characterization and bioactivity of neutral polysaccharides from different sources of Polygonatum Mill

Wang

Jiang

Jin³

et al. 2022

Biopolymers

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Polygonati rhizoma (PR), a traditional medical and edible product, is rich in polysaccharides and exhibits physiological activity, including antioxidant, hypoglycemic and hypolipidemic properties. Neutral polysaccharides have been reported to be one of the main active ingredients of Polygonatum, with many of these fractions being responsible for the biological activity. This behavior was shown to be closely connected to the chemical structure, monosaccharide composition, and glycosidic bond type. There are few reports on the chemical constituents of the neutral polysaccharides from different sources of PR. In this study, neutral polysaccharides of PR from four different regions of China (Chun'an (Zhejiang), Xixia (Henan), Danfeng (Shanxi), and Pan'an (Zhejiang)), named CAZJ, XXHN, DFSX, and PAZJ, respectively, were isolated by anion‐exchange and gel‐permeation chromatography. Structures of the four polysaccharides were investigated. The results showed that all of them were mainly glucose and mannose, while the monosaccharide composition and content of polysaccharides from different sources varied. The molecular weights of CAZJ, XXHN, DFSX, and PAZJ were 14.119, 22.352, 18.127, and 15.699 kDa, respectively. Infrared spectra illustrated the existence of α‐glycosidic bond and β‐glycosidic bond in the polysaccharides. CAZJ, XXHN, and DFSX possessed a pyranose ring structure, whereas PAZJ had a furanose ring structure. Congo red test indicated that XXHN, DFSX, and PAZJ had a triple‐helix structure. X‐ray diffraction showed that the polysaccharides consisted of crystalline and amorphous regions. All four polysaccharides exhibited different degrees of antioxidant and hypoglycemic activities with a dose‐dependent manner in the 1.0–10.0 mg/mL concentration range. Correlation analysis revealed that the bioactivities of polysaccharides was significantly related to monosaccharide composition, uronic acid, and protein content. The results suggested that neutral polysaccharides could be used as potential natural antioxidants and hypoglycemic agents for functional and nutraceutical applications.

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Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum

Cited by 91 publications

References 38 publications

Purification and Biological Activities of Enzymatically Degraded Sargassum fusiforme Polysaccharides

Purification and Biological Activities of Enzymatically Degraded Sargassum fusiforme Polysaccharides

Structural analysis and anti‐obesity effect of Polygonatum cyrtonema polysaccharide against obesity induced by high‐fat diet in mice

Structure characterization and bioactivity of neutral polysaccharides from different sources of Polygonatum Mill

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