Purification, Partial Characterization and Immobilization of a Mannose-Specific Lectin from Seeds of Dioclea lasiophylla Mart.

Abstract

Abstract:Lectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on a Sephadex ® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and was inhibited by monosaccharides ( D -mannose and α-methyl-Dmannoside) and glycoproteins (ovalbumin and fetuin). Similar to other Diocleinae lectins, DlyL has three chains, α, β and γ, with mass of 25,569 ± 2, 12,998 ± 1 and OPEN ACCESSMolecules 2013, 18 10858 12,588 ± 1 Da, respectively, and has no disulfide bonds. The hemagglutinating activity of DlyL was optimal in pH 8.0, stable at a temperature of 70 °C and decreased in EDTA solution, indicating that lectin activity is dependent on divalent metals. DlyL exhibited low toxicity on Artemia sp. nauplii, but this effect was dependent on the concentration of lectin in solution. DlyL immobilized on cyanogen bromide-activated Sepharose ® 4B bound 0.917 mg of ovalbumin per cycle, showing the ability to become a tool for glycoproteomics studies.

Purification and molecular characterization of a novel mannose‐specific lectin from Dioclea reflexa hook seeds with inflammatory activity

“…The Artemia lethality test has been used successfully to determine the toxicity of bioactive compounds that have a variety of pharmacological activities, including anticancer agents, antivirals, insecticides, pesticides, and anti‐HIV compounds (Carballo et al ., ; Pervin et al ., ; Ho et al ., ). Other studies evaluated the toxic effect of some ConA‐like lectins, and they reported LC 50 values ranging from 45.85 to 376.48 µg/ml (Pinto‐Junior et al ., ; Arruda et al ., ). In comparison with similar lectins, DrfL exhibited low toxicity against Artemia sp.…”

“…The agglutination of DrfL was inhibited by α ‐methyl‐ d ‐mannoside, d ‐mannose, and d ‐glucose (Table ). This specificity is similar to that of other lectins in the Dioclea genus (Nascimento et al ., ; Pinto‐Junior et al ., ).…”

“…Many lectins have been purified from seeds of species of the subtribe Diocleinae, such as Canavalia brasiliensis (Moreira and Cavada, ), Dioclea lasiophylla (Pinto‐Junior et al ., ), Canavalia oxyphylla (Santiago et al ., ), and Canavalia virosa (Osterne et al ., ). Due to the broad spectrum of biological properties, these proteins can be utilized in several biotechnological/biomedical applications (El‐Aassar et al ., ).…”

Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds

“…The lectin from Dioclea lasiophylla seeds is another example of lectins presenting bands of different molecular weights. DlyL is composed of three different chains with different molecular weights and no bisulfide bonds present (Pinto-Júnior et al, 2013).…”

Antiproliferative activity of cytotoxic tuber lectins from Solanum tuberosum against experimentally induced Ehrlich ascites carcinoma in mice

“…A lectin from Curcuma amarissima rhizomes showed in vitro antiproliferative activity against a breast cancer cell line (BT 474) with an LC 50 value of approximately 21.1 μg (Kheeree et al, 2010). Highly toxic lectins were isolated from Abrus pulchellus and Mulberry seeds having LC 50 values of 3.5 mg/ml and 21.87 μg/ml whereas other lectins from the seeds of Dioclea lasiophylla and tubers of Nymphaea nouchali had much lower values like 45.85 and 120 μg/ml, respectively (Ramos et al, 1998;Absar et al, 2005;Pinto-Junior et al, 2013;Kabir et al, 2012). Presence of a non-toxic lectin from Sebastiania jacobinensis bark to Artemia nauplii is also reported (Vaz et al, 2010).…”