Purification of Xylanases

Bajpai, Pratima

doi:10.1016/b978-0-12-801020-4.00006-8

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…Xylanases are usually purified by a multi-step process that involve non-specific techniques. A higher number of steps adopted in the purification process results in lower yields of the enzyme in most cases [24]. Purification of xylanase needs to be achieved in order to perform complete biochemical analysis and molecular studies.…”

Section: Resultsmentioning

confidence: 99%

Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Ravindran¹,

Williams²,

Jaiswal³

2019

Foods

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In this study, spent coffee waste (SCW) was used as the sole carbon source for xylanase production in solid state fermentation mode using Aspergillus niger. A Box–Behnken design was constructed using three parameters viz. temperature, initial moisture content, and log number of spores to determine the optimal fermentation condition. The best fermentation conditions for xylanase production were found to be incubation at 30 °C with an initial moisture content of 70% and using an inoculum of 6.5 × 106 spores/g of dry SCW. Furthermore, the design of experiments revealed that maintaining a medium composition of 0.2 g of yeast extract, 0.04 g of K2HPO4, and 0.03 g of MgSO4 increased xylanase production. Under optimised solid-state fermentation conditions an enzyme activity of 6495.6 IU/g of dry SCW was recorded, which was approximately 1.39-fold higher than that of control (4649 IU/g of dry SCW). The efficacy of the purified xylanase as a juice enrichment agent for strawberry, blueberry, and raspberry pulp was tested.

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Section: Resultsmentioning

confidence: 99%

Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Ravindran¹,

Williams²,

Jaiswal³

2019

Foods

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“…Enzymes are needed in purified form to obtain information about their amino acid sequence, biochemical functions, and evolutionary relationships between proteins in diverse organisms [ 94 ]. For the purification of chitinase, the culture filtrate is generally precipitated using the ammonium sulfate precipitation method, followed by the use of various chromatography techniques for further purification ( Table 4 ).…”

Section: Microbial Fermentation For Chitinase Productionmentioning

confidence: 99%

Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications

Poria

Rana

Kumari

et al. 2021

Biology

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Chitinases are a large and diversified category of enzymes that break down chitin, the world’s second most prevalent polymer after cellulose. GH18 is the most studied family of chitinases, even though chitinolytic enzymes come from a variety of glycosyl hydrolase (GH) families. Most of the distinct GH families, as well as the unique structural and catalytic features of various chitinolytic enzymes, have been thoroughly explored to demonstrate their use in the development of tailor-made chitinases by protein engineering. Although chitin-degrading enzymes may be found in plants and other organisms, such as arthropods, mollusks, protozoans, and nematodes, microbial chitinases are a promising and sustainable option for industrial production. Despite this, the inducible nature, low titer, high production expenses, and susceptibility to severe environments are barriers to upscaling microbial chitinase production. The goal of this study is to address all of the elements that influence microbial fermentation for chitinase production, as well as the purifying procedures for attaining high-quality yield and purity.

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“…Many studies have evaluated the properties of various enzymes in their crude form; however, purification of enzymes has been noted to be essential for detailed biochemical, biophysical and molecular characterization. Although the ultimate degree of purity of a specific protein or enzyme is a function of its end use, purification to homogeneity is highly desirable, despite being highly expensive, laborious and time-consuming [ 12 ]. It also helps determine the enzyme’s structure at the primary, secondary, tertiary and quaternary levels.…”

Section: Introductionmentioning

confidence: 99%

Beauveria bassiana Xylanase: Characterization and Wastepaper Deinking Potential of a Novel Glycosyl Hydrolase from an Endophytic Fungal Entomopathogen

Amobonye

Bhagwat

Singh

2021

JoF

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Beauveria bassiana is an entomopathogenic fungus widely used as a biopesticide for insect control; it has also been shown to exist as an endophyte, promoting plant growth in many instances. This study highlights an alternative potential of the fungus; in the production of an industrially important biocatalyst, xylanase. In this regard, Beauveria bassiana SAN01 xylanase was purified to homogeneity and subsequently characterized. The purified xylanase was found to have a specific activity of 324.2 Umg−1 and an estimated molecular mass of ~37 kDa. In addition, it demonstrated optimal activity at pH 6.0 and 45 °C while obeying Michaelis–Menton kinetics towards beechwood xylan with apparent Km, Vmax and kcat of 1.98 mgmL−1, 6.65 μM min−1 and 0.62 s−1 respectively. The enzyme activity was strongly inhibited by Ag2+ and Fe3+ while it was significantly enhanced by Co2+ and Mg2+. Furthermore, the xylanase was shown to effectively deink wastepaper at an optimal rate of 106.72% through its enzymatic disassociation of the fiber-ink bonds as demonstrated by scanning electron microscopy and infrared spectroscopy. This is the first study to demonstrate the biotechnological application of a homogeneously purified glycosyl hydrolase from B. bassiana.

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Purification of Xylanases

Cited by 7 publications

References 34 publications

Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications

Beauveria bassiana Xylanase: Characterization and Wastepaper Deinking Potential of a Novel Glycosyl Hydrolase from an Endophytic Fungal Entomopathogen

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