Purification of viable ciliated cuboidal ependymal cells from rat brain

Manthorpe, C. Marston; Wilkin, Graham P.; Wilson, John E.

doi:10.1016/0006-8993(77)90818-6

Cited by 26 publications

(6 citation statements)

References 27 publications

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“…In particular, the reactivity of CD133 pAb in this study was consistent with the report that CD133 pAb reacts with cell membrane areas on the apical side of ependymal cells (Weigmann et al 1997;Marzesco et al 2005). Ependymal cells line the ventricular system such as LVs, third ventricles, cerebral aqueducts, fourth ventricles, and central canals of the spinal cord, and their morphology widely differs depending on their loci (Manthrope et al 1977). Their main functions in adult brains are secretion and/or absorption of cerebrospinal fluid components (Manthrope et al 1977).…”

Section: Cellular Characteristics Of Inca Mab 1 Cells Isolated From Nsupporting

confidence: 90%

“…Ependymal cells line the ventricular system such as LVs, third ventricles, cerebral aqueducts, fourth ventricles, and central canals of the spinal cord, and their morphology widely differs depending on their loci (Manthrope et al 1977). Their main functions in adult brains are secretion and/or absorption of cerebrospinal fluid components (Manthrope et al 1977). A great deal of recent literatures has reported that ependymal cells work as NSCs in adult rodent brains (Pfenninger et al 2007;Coskun et al 2008;Gleason et al 2008;Pfenninger et al 2011) and in the chordate larval nervous system (Horie et al 2011).…”

Section: Cellular Characteristics Of Inca Mab 1 Cells Isolated From Nmentioning

confidence: 99%

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A Novel Monoclonal Antibody Against Neuroepithelial and Ependymal Cells and Characteristics of Its Positive Cells in Neurospheres

et al. 2015

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There are still few useful cell membrane surface antigens suitable for identification and isolation of neural stem cells (NSCs). We generated a novel monoclonal antibody (mAb), designated as mAb against immature neural cell antigens (INCA mAb), which reacted with the areas around a lateral ventricle of a fetal cerebrum. INCA mAb specifically reacted with neuroepithelial cells in fetal cerebrums and ependymal cells in adult cerebrums. The recognition molecules were O-linked 40 and 42 kDa glycoproteins on the cell membrane surface (gp40 INCA and gp42 INCA). Based on expression pattern analysis of the recognition molecules in developing cerebrums, it was concluded that gp42 INCA was a stage-specific antigen expressed on undifferentiated neuroepithelial cells, while gp40 INCA was a cell lineage-specific antigen expressed at the stages of differentiation from neuroepithelial cells to ependymal cells. A flow cytometric analysis showed that fetal and young adult neurospheres were divided into INCA mAb(-) CD133 polyclonal antibody (pAb)(-), INCA mAb(+) CD133 pAb(-), and INCA mAb(+) CD133 pAb(+) cell populations based on the reactivity against INCA mAb and CD133 pAb. The proportion of cells having the neurosphere formation capability in the INCA mAb(+) CD133 pAb(+) cell population was significantly larger than that of undivided neurospheres. Neurospheres formed by clonal expansion of INCA mAb(+) CD133 pAb(+) cells gave rise to neurons and glial cells. INCA mAb will be a useful immunological probe in the study of NSCs.

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Section: Cellular Characteristics Of Inca Mab 1 Cells Isolated From Nsupporting

confidence: 90%

Section: Cellular Characteristics Of Inca Mab 1 Cells Isolated From Nmentioning

confidence: 99%

A Novel Monoclonal Antibody Against Neuroepithelial and Ependymal Cells and Characteristics of Its Positive Cells in Neurospheres

et al. 2015

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“…Because ependymal cells are organized into a simple epithelium they are easy to identify in whole brain studies. Cell isolation and tissue culture techniques have also been applied to ependymal cells in order to study their metabolic characteristics (Majocha et al, 1982;Manthorpe et al, 1977;Weibel et al, 1986). Isolation of ependymal cells by stripping from the ventricle surface with subsequent culturing yields in a stable non-proliferating epithelium which is not overgrown by other cell types (Gee et al, 1993).…”

Section: Cellular Protein Expressionmentioning

confidence: 99%

The ependyma: A protective barrier between brain and cerebrospinal fluid

Bigio

1995

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286

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This review summarizes the current scientific literature concerning the ependymal lining of the cerebral ventricles of the brain with an emphasis on selective barrier function and protective roles for the common ependymal cell. Topics covered include the development, morphology, protein and enzyme expression including reactive changes, and pathology. Some cells lining the neural tube are committed at an early stage to becoming ependymal cells. They serve a secretory function and perhaps act as a cellular/axonal guidance system, particularly during fetal development. In the mature mammalian brain ependymal cells possess the structural and enzymatic characteristics necessary for scavenging and detoxifying a wide variety of substances in the CSF, thus forming a metabolic barrier at the brain-CSF interface.

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“…We have a particular interest in viral receptors on nervous system tissue and techniques exist to obtain freshly isolated cells from the central nervous system, such as oligodendrocytes (Snyder et d . , 1980), astrocytes (Farooq and Norton, 1978), ependymal cells (Manthorpe et al, 1977), or in some instances neurons (Farooq and Norton, 1978). Freshly isolated cells can then be used to identify viruses which have an affinity for them.…”

Section: Specificity Of Cell Receptor Sites For Virusesmentioning

confidence: 99%