Purification of Toxoplasma by Means of Sonic Vibration and Tryptic Digestion *

Tsunematsu, Yukinori

doi:10.4269/ajtmh.1960.9.556

Cited by 9 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are several published reports on purification of Toxoplasma tachyzoites by passing the parasite through a 25‐gauge needle, filtration through a 3‐μm pore size, sucrose gradient density centrifugation (Garberi et al., 1990; Khan & Grigg, 2017). Toxoplasma tachyzoite prurification can also be achieved by sonic vibration and trypsin digestion (Tsunematsu, 1960) or by a sintered glass filter. These procedures are often complex and pose scale‐up challenges.…”

Section: Resultsmentioning

confidence: 99%

A rapid and simple single‐step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites

El‐Ashram

Salama

et al. 2020

Veterinary Medicine & Sci

View full text Add to dashboard Cite

This study describes a simple method for the large‐scale isolation of pure Toxoplasma gondii tachyzoites and bradyzoites. T. gondii tachyzoites were obtained from infected human foreskin fibroblasts (HFFs) and peritoneal exudates of mice, while tissue cysts containing bradyzoites were collected from chronically infected mice. Harvested cells and brain tissues were incubated in Hanks balanced salt solution (HBSS), containing 0.25% trypsin and 0.5% taurodeoxycholic acid (TDC) for 5 min. Subsequent washes in phosphate buffered saline (PBS) were conducted, and the cell viability of the preparations was good, as determined by flow cytometry and ability to reinfect HFF cells and propagate in mice. The purification procedure allowed for a rapid preparation of pure T. gondii tachyzoites and bradyzoites in sufficient quantity that can be used for downstream procedures. The advantage of the new method is that it is convenient and inexpensive.

show abstract

Section: Resultsmentioning

confidence: 99%

A rapid and simple single‐step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites

El‐Ashram

Salama

et al. 2020

Veterinary Medicine & Sci

View full text Add to dashboard Cite

show abstract

“…La trypsinisation a démontré être une méthode très efficace pour purifier les toxo plasmes (11). Par gramme, de poids humide, de culot d'ascite de souris (toxoplasme plus cellules) on a pu obtenir 295 mg, de poids humide, de toxoplasmes purs.…”

Section: Résultatsunclassified

Nouvel antigène pour la réaction de fixation du complément pour le diagnostic de la Toxoplasmose

Yanovsky

Traversa

Schmuñis

et al. 1968

Ann. Parasitol. Hum. Comp.

View full text Add to dashboard Cite

Dans le présent travail est décrite une nouvelle technique de préparation des antigènes de Toxoplasma gondii, sous l'effet de la pression, à partir de toxoplasmes purifiés au moyen de la trypsine. Le toxoplasme a été soumis à des pressions de 5.000, 10.000, 15.000, 20.000 et 25.000 PSI, cette dernière (25.000 PSI) étant la seule capable d'effectuer à 100 % la rupture des toxoplasmes. L'antigène Fixateur du Complément a été obtenu en soumettant à une pression de 25.000 PSI une suspension de toxoplasmes purifiés au moyen de la trypsine utilisée à la concentration de 20 mg par ml. Grâce à cette technique il est possible d'obtenir un antigène de titre supérieur ayant une spécificité et une sensibilité égales à celles obtenues par d'autres procédés.

show abstract

Toxoplasma gondii: Trypsin treatment of trophozoites for enhanced immune cytolysis in the dye test

Suzuki

Tsunematsu

1973

Experimental Parasitology

View full text Add to dashboard Cite

Purification of Toxoplasma by Means of Sonic Vibration and Tryptic Digestion *

Cited by 9 publications

References 0 publications

A rapid and simple single‐step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites

A rapid and simple single‐step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites

Nouvel antigène pour la réaction de fixation du complément pour le diagnostic de la Toxoplasmose

Toxoplasma gondii: Trypsin treatment of trophozoites for enhanced immune cytolysis in the dye test

Contact Info

Product

Resources

About