Purification of Protochlorophyllide Holochrome

Abstract

A bstract. Phototransformable protochlorophyllide holochrome was prepared from etiolated bean leaves. The detergent Triton X-100 in the presence of glycerol and tricine-KOH buffer (p14 8) enlhanced the extractability, specific activity, and phototransformability of the holochrome. Purification was achieved by polyethylene glycol-6000 precipitation and hydroxylapatite, DEAE-cellulose, and agarose chromatography.

Pigment-Protein Complexes of Illuminated Etiolated Leaves

“…1) (20). The failure of holochrome preparations (1,14,23) and unsupplemented purified membranes (Fig. 2), to effect the NADPH-dependent Chlide 678 to Chlide 684 transition, can similarly be explained on the basis of a lack of NADPH in such material, this having been washed away during preparation.…”

Events Surrounding the Early Development of Euglena Chloroplasts

“…In Table I (39). Red and blue were normalized to green taken as 1 for both relative effectiveness and expected effectiveness.…”

Relationship between Photoconvertible and Nonphotoconvertible Protochlorophyllides

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ABSTRACTTwo forms of protochlorophyllide are found in dark-grown bean (Phaseolus vulgaris, var (13,14,17).ALA treatment of the leaves of dark-grown seedlings causes accumulation of a form of PChld with an absorbance peak

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