Events Surrounding the Early Development of <i>Euglena</i> Chloroplasts

Holowinsky, Andrew W.; Schiff, Jerome A.

doi:10.1104/pp.45.3.339

Cited by 87 publications

(51 citation statements)

References 41 publications

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“…Preillumination for 2 h followed by a 12-h dark period (prior) to exposure of the cells to continuous light results in potentiation or elimination of the lag phase in Chl formation (18). Under these conditions there is no synthesis of Cyt-552 during the dark period but the synthesis starts rapidly on exposure of the cells to continuous light (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Antibiotics were present during entire period of chloroplast development. Cells grown in the dark were transferred to pH 6.8 resting medium, incubated 3 days in darkness, and then divided into three aliquots: streptomycin (0.5 mg/ml) was added to one, 16 h before being placed in the light (0); cytoheximide (10 yg/ml) was added to another one on being placed in the light (0); and a third was illuminated without additions (x) (18).…”

Section: Resultsmentioning

confidence: 99%

“…bacillaris Cori, Strain Z Pringsheim and several mutants derived from them were grown at 26 C under constant shaking in light or darkness on Hutner's medium (pH 3.5) (14,32). For studies of light-induced chloroplast development in nondividing cells, dark-grown cells were transferred to pH 6.8 resting medium (18,32) and after 3 days the cultures were placed in the light to initiate chloroplast development. Cell counts (38), Chl determinations; (32), and antibiotic treatments (4) were done as described previously.…”

Section: Methodsmentioning

confidence: 99%

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Events Surrounding the Early Development of Euglena Chloroplasts

Freyssinet¹,

Harris²,

Nasatir³

et al. 1979

Plant Physiol.

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Lack of a suitable assay has thwarted attempts to measure cytochrome c-552 in dark-grown wild type cells of Euglena gracilis var. bacillanis in mutants and in other situations where the concentrations are low. Purification methods are described based on electrofocusing which provide a cytochrome c-552 preparation homogeneous enough to elicit a single reactive antibody in rabbits; this antibody is then used as a specific and sensitive assay for cytochrome c-552. Dark-grown cells of wild type and of mutants O,BS, 02BX, GLBU and P,BXL (which make normal sized chloroplasts with abnormal internal structure in the light) have 0.02 to 0

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Events Surrounding the Early Development of Euglena Chloroplasts

Freyssinet¹,

Harris²,

Nasatir³

et al. 1979

Plant Physiol.

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“…This is shown, among other things, by the fact that aplastidic Euglena mutants are perfectly viable provided they are grown in the presence of suitable organic nutrients. In Euglena, Chl synthesis is regulated by Pchlide feedback at the level of ALA formation (9,19). At the opposite extreme, in cucumber cotyledons, we have found no evidence for Pchlide feedback regulation (12), but only for phytochrome regulation.…”

Section: Discussionmentioning

confidence: 99%

Regulation of 5-Aminolevulinic Acid (ALA) Synthesis in Developing Chloroplasts

Huang¹,

Bonner²,

Castelfranco³

1989

Plant Physiol.

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When dark-grown cucumber (Cucumis sativus L.) seedlings previously exposed to white light for 20 hours were retumed to darkness, the ability of isolated chloroplasts to synthesize 5-aminolevulinic acid dropped by approximately 70% within 1 hour. The seedlings were then exposed to light, and the synthetic ability of the isolated chloroplasts was determined. Restoration of the synthetic capacity was promoted by continuous white or red light of moderate intensity. Intermittent red light was also effective. Blue and far-red light did not restore the synthetic capability. Blue light given after a red pulse did not enhance the effect of the red light. Far-red light given immediately after each red pulse prevented the stimulation due to intermittent red light. Restoration of the biosynthetic activity by in vivo light treatments was inhibited by cycloheximide indicating the requirement for translation on 80 S ribosomes for the in vivo light response. These findings suggest that the majority of the plastidic 5-aminolevulinic acid synthesis is under phytochrome regulation.

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“…As with plant tissues, Euglena cells grown in complete darkness fail to accumulate Chl, and instead form small quantities of Pchl(ide). Exposure to light brings about the reduction ofthese pigments to Chl(ide), and in continuous light this is followed by a phase of rapid Chl accumulation (17) and transformation of developing plastids into photosynthetically functional chloroplasts (34).…”

mentioning

confidence: 99%