Purification of Protein from Marine Edible Oyster Crassostrea madrasensis for Bactericidal Potency

Abstract

Nowadays, the pharmaceutical market is growing rapidly and continuously in worldwide but, still the demand for new drug discovery is encouraged. Because, the growth of numbers drug resistant infectious disease and more upcoming disorders to human and animals. In general, the marine animals especially mollusks and their compounds constitute a practically unlimited resource of new active substances. Hence, the present study was carried out to determine the bactericidal activity of Crassostrea madrasensis protein against human pathogens. The edible Oyster Crassostrea madrasensis was collected from Rayapuram lnading centre, Tamil Nadu, India. Immediately it was extracted by using phosphate buffer at three different pH (4, 7 and 9) and all the extracts were screened against six different human pathogens such as Vibrio cholerae, V. parahaemolyticus, Salmonella sp, Shigella sp, Streptococcus sp and Staphylococcus sp by agar well diffusion assay. After 24 hrs of incubation the maximum inhibitory effect was observed against Vibrio parahaemolyticus, Streptococcus sp and Staphylococcus sp and the minimum inhibitory effect was observed against Vibrio cholerae, Salmonella sp and Shigella sp respectively. Whereas checking the minimal inhibitory concentration (MIC), the crude protein extract of Crassostrea madrasensis was inhibited the bacterial strains with the minimum inhibitory concentration of not less than 0.1ml (100¼l). The molecular weight of the crude protein was found from 12.2 to 74.2 kDa and the total protein content of phosphate buffer crude extract of Crassostrea madrasensis was found to be 312 ¼g/ mg. From, the results, the work has suggested to use this commercially available and protein rich (bactericidal) oyster in therapeutics for the development of novel antibiotics against multiple drug resistance (MDR) pathogenic microbes.

Proteins Profiling and Structural Elucidation by in silico Methods in Potamidid Snails

“…The supernatant was obtained, freeze-dried and lyophilized. The lyophilized crude extract was reconstituted in 9.5 mL of phosphate buffer saline (PBS) at pH 7.0 for further proteomic analysis 6 . The protein spots on the destined gel were quantitated using gel doc.…”

Framework and Implementation of Marine Dumping Area’s Monitoring System Based on GPRS

“…For checking the expansive measure of remote gadgets crosswise over areas, GPRS is the most ideal information transmission mode. [4] for cleaning process. Design of acoustic modem for autonomous underwater vehicles [5] was proposed for the identification of the vehicles or ships by positioning method.…”

“…The Presentation Layer that is monitor is composed of deployment tools, monitoring man-machine interface, alert and report table forms. Mainly, the GIS (Geographical Information System) techniques are used in a graphic interface to realise the monitoring field positioning [4].…”

“…By using the integrated development environment of SPCE3200's S + core IDE, modules are programmed oriented to GPRS. Utilizing SPCE3200's API functions as well as by using C programming language, the coordinator node controls the SPCE3200 to send data to GPRS module [4]. b) GPRS The centre connects to RTU GPRS Modem unit by dialling to establish the PPP connecting state.…”