Purification of lentil lectins using preparative electrophoresis

Wenger, Pierre; Heydt, Ariane; Egen, Ned B.; Long, Terry D.; Bier, M.

doi:10.1016/s0021-9673(01)82121-x

Cited by 15 publications

(6 citation statements)

References 12 publications

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“…FF‐ITP and ZE, in contrast, are at present of minor importance for protein separation. Nevertheless, several publications deal with the optimization of the electrophoretic conditions in both FFE modes using selected model proteins 10, 44–52. A few ZE protocols for the separation of individual proteins or even peptides from differing cellular sources have been published 53–68.…”

Section: Separation Of Proteins and Peptidesmentioning

confidence: 99%

Free‐flow electrophoresis in the proteomic era: A technique in flux

2010

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Since its introduction five decades ago, free-flow electrophoresis (FFE) has been mainly employed for the isolation and fractionation of cells, cell organelles and protein mixtures. In the meantime, the growing interest in the proteome of these bio-particles and biopolymers has shed light on two further facets in the potential of FFE, namely its applicability as an analytical tool and sensor. This review is intended to outline recent innovations, FFE has gained in the proteomic era, and to point out the valuable contributions it has made to the analysis of the proteome of cells, sub-cellular organelles and functional protein networks.

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Section: Separation Of Proteins and Peptidesmentioning

confidence: 99%

Free‐flow electrophoresis in the proteomic era: A technique in flux

2010

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“…purity of the lectins was not achieved with FFE alone, the collected fractions could be further purified by using RIEF; thus, all remaining traces of acidic contaminants were removed. These two preparative electrophoresis steps could replace a chromatographic procedure in which two ion-exchange steps for the purification of lectins from lentil seeds are used (39).…”

Section: Applicationsmentioning

confidence: 99%

Free-flow electrophoresis as a preparative separation technique

Roman¹,

Brown²

1994

Anal. Chem.

107

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“…The technique has been successfully applied to the separation of small ions [5], enzymes [6], proteins [7], cells [8], and has evolved into a promising method of continuous separation for biomolecules and DNA samples [9].…”

Section: Introductionmentioning

confidence: 99%

An online fiber-optic UV-visible detector for continuous free-flow electrophoresis

et al. 2005

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A PC-controlled, scanning online UV detector for continuous free-flow electrophoresis (CFFE) was designed to allow for single UV wavelength monitoring across a 1-D array of 48 longitudinal flow cells interfaced to a CFFE apparatus. In the detection scheme, the UV light is sequentially passed through each of the flow cells. The design integrates online acquisition of absorbance spectra of components separated by CFFE. Benzoic acid standard solutions were used to examine the performance of the detector. Chloroquine diphosphate and 4-nitrophenol were used as test solutes to determine the detector's ability to distinguish analytes separated in the CFFE.

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Purification of lentil lectins using preparative electrophoresis

Cited by 15 publications

References 12 publications

Free‐flow electrophoresis in the proteomic era: A technique in flux

Free‐flow electrophoresis in the proteomic era: A technique in flux

Free-flow electrophoresis as a preparative separation technique

An online fiber-optic UV-visible detector for continuous free-flow electrophoresis

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