Purification of human monocytes on gelatin-coated surfaces

Hassan, Nassef F.; Campbell, Donald E.; Douglas, Steven D.

doi:10.1016/0022-1759(86)90415-1

Cited by 111 publications

(67 citation statements)

References 9 publications

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“…Numerous blood cells (stem, progenitor, or committed mature lineages) express the integrin receptors for fibronectin and attach to plates coated with this molecule. In fact, monocytes are known to be highly enriched from peripheral blood MNCs when plated on fibronectin-coated dishes, and adherent monocytes cultured in media containing endothelial growth factors are known to express a variety of proteins typically thought to be reserved for endothelial cells (von Willebrand factor, endothelial nitric oxide synthase, CD31, CD144, and vascular endothelial growth factor 2 receptor [KDR]) (Hassan et al 1986;Schmeisser et al 2001Schmeisser et al , 2003. Recent proteomic and mRNA profiling analyses have indicated that human peripheral blood MNCs cultured as adherent cells on fibronectin-coated dishes in culture medium with added endothelial growth factors display a gene expression pattern that highly resembles hematopoietic (particularly cultured myeloid and T lymphoid cells) but not human endothelial cells (Medina et al 2010).…”

Section: Methods To Define Human Epcsmentioning

confidence: 99%

Human Endothelial Progenitor Cells

Yoder

2011

Cold Spring Harbor Perspectives in Medicine

345

252

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Section: Methods To Define Human Epcsmentioning

confidence: 99%

Human Endothelial Progenitor Cells

Yoder

2011

Cold Spring Harbor Perspectives in Medicine

345

252

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“…Heparinized blood samples were identified as HIV antibody negative by anonymous testing with the enzyme-linked immunosorbent assay method (Coulter Immunology, Hialeah, FL). Monocytes were purified according to a previously described technique (Hassan et al, 1986). In brief, heparinized blood was separated by centrifugation over lymphocyte separation medium (Organon Teknika, Durham, NC) at 400 to 500g for 45 min.…”

Section: Methodsmentioning

confidence: 99%

Methylnaltrexone Antagonizes Opioid-Mediated Enhancement of HIV Infection of Human Blood Mononuclear Phagocytes

Ho¹,

Guo²,

Yuan³

et al. 2003

J Pharmacol Exp Ther

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Opioid abuse has been postulated as a cofactor in the immunopathogenesis of human immunodeficiency virus (HIV) infection and AIDS. We and others have recently demonstrated that opioid enhances HIV infection of human macrophages through modulation of ␤-chemokines and the CCR5 receptor and that this effect is reversed by naltrexone, a tertiary opioid antagonist. Tertiary opioid antagonists cannot be used in opioiddependent patients because they precipitate withdrawal or reversal of analgesia. We determined whether the quaternary opioid antagonist methylnaltrexone (MNTX), now in phase III clinical trials for opioid-induced constipation, reverses the opioid-mediated enhancement of HIV infection of macrophages at clinically relevant doses. MNTX completely abrogated morphine-induced HIV Bal strain infection of macrophages. MNTX also inhibited the R5 strain (ADA) envelope-pseudotyped HIV replication induced by morphine. Furthermore, MNTX abolished morphine-mediated up-regulation of CCR5 receptor expression. The ability of MNTX to block opioid-induced CCR5 expression and HIV replication at clinically relevant doses may have additional benefit for opioid abusers with HIV infection, or patients with AIDS pain receiving opioids.

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“…The blood samples were identified as HIV-1 antibody negative by anonymous testing with the ELISA method (Coulter). Monocytes were purified according to our previously described techniques (23,24).…”

Section: Methodsmentioning

confidence: 99%