Human Endothelial Progenitor Cells

Yoder, Mervin C.

doi:10.1101/cshperspect.a006692

Cited by 345 publications

(271 citation statements)

References 99 publications

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“…In addition, oxidative stress-induced endothelial dysfunction is involved in ischemia/reperfusion injury (18). EPCs are crucial in the angiogenesis of ECs and it has been suggested that they serve an important role in angiogenesis occurring under hypoxia/ischemia (19,20). Hypoxia/ischemia stimulates EPCs to migrate from the bone marrow into the peripheral blood, adhere to the endothelium at sites of hypoxia/ischemia and participate in new vessel formation by differentiating into ECs (21).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-138 inhibits hypoxia-induced proliferation of endothelial progenitor cells via inhibition of HIF-1α-mediated MAPK and AKT signaling

Zhou

Zeng

Xiong

2017

Experimental and Therapeutic Medicine

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Abstract. Endothelial progenitor cells (EPCs) participate in angiogenesis by differentiating into endothelial cells (ECs)and may be developed to treat ischemia/reperfusion injury. MicroRNAs (miRs) are a type of non-coding RNA that are 18-25 nucleotides in length and serve a role in angiogenesis. It has been demonstrated that miR-138 regulates hypoxia-induced EC dysfunction. However, to the best of our knowledge, the exact role of miR-138 in the regulation of hypoxia-induced EPCs has not previously been reported. In the present study, data collected from an MTT assay indicated that hypoxia treatment enhanced EPC proliferation, which was accompanied by an upregulation of hypoxia-inducible factor 1α (HIF-1α) expression. miR-138 overexpression inhibited hypoxia-induced EPC proliferation and induced cell cycle arrest at the G1 stage. A mechanistic investigation revealed that miR-138 negatively regulated HIF-1α protein levels but did not affect HIF-1α mRNA levels in EPCs. Moreover, results from a dual luciferase reporter assay demonstrated that HIF-1α was a direct target of miR-138 in EPCs. Furthermore, upregulation of miR-138 suppressed the hypoxia-induced upregulation of HIF-1α. Downstream factors of HIF-1α were also investigated and it was observed that the upregulation of miR-138 inhibited the hypoxia-induced upregulation of vascular endothelial growth factor, as well as the activity of mitogen-activated protein kinase and AKT signaling in EPCs. In summary, the present study suggested that miR-138 inhibits hypoxia-induced EPC proliferation, possibly by inhibiting HIF-1α-mediated signaling.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-138 inhibits hypoxia-induced proliferation of endothelial progenitor cells via inhibition of HIF-1α-mediated MAPK and AKT signaling

Zhou

Zeng

Xiong

2017

Experimental and Therapeutic Medicine

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“…Interestingly, there is a report suggesting that adipose stromal cells may enhance endothelial differentiation of progenitor cells (Rubina et al, 2009). However, another essential marker for endothelial progenitor cells, CD309 (VEGFR2) (Yoder, 2012), showed very low expression in the six hASC lines that were analyzed in this study. Therefore, the presence of an endothelial progenitor cell population in the stromal-vascular fraction needs to be studied further.…”

Section: Discussionmentioning

confidence: 66%

Human vascular model with defined stimulation medium – a characterization study

Huttala

2015

ALTEX

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SummaryThe formation of blood vessels is a vital process in embryonic development and in normal physiology. Current vascular modelling is mainly based on animal biology leading to species-to-species variation when extrapolating the results to humans. Although there are a few human cell based vascular models available, these assays are insufficiently characterized in terms of culture conditions and developmental stage of vascular structures. Therefore, well characterized vascular models with human relevance are needed for basic research, embryotoxicity testing, development of therapeutic strategies and for tissue engineering. We have previously shown that the in vitro vascular model based on co-culture of human adipose stromal cells (hASC) and human umbilical vein endothelial cells (HUVEC) is able to induce an extensive vascular-like network with high reproducibility. In this work we developed a defined serum-free vascular stimulation medium (VSM) and performed further characterization in terms of cell identity, maturation and structure to obtain a thoroughly characterized in vitro vascular model to replace or reduce corresponding animal experiments. The results showed that the novel vascular stimulation medium induced an intact and evenly distributed vascular-like network with morphology of mature vessels. Electron microscopic analysis assured the three-dimensional microstructure of the network containing lumen. Additionally, elevated expression levels of the main human angiogenesis-related genes were detected. In conclusion, with the newly defined medium the vascular model can be utilized as a characterized test system for chemical testing as well as in creating vascularized tissue models.

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“…They have also been used as a biomarker or as a cell therapy in various clinical trials to treat patients with heart disease, diabetes, peripheral arterial disease, pulmonary disease and cancer despite the ambiguity in fully characterizing it. Thus, EPCs have many functions and the area of vascular biology involving endothelial cells is slowly emerging in treating various life-threatening pathologies [1].…”

Section: Efficient Methods Of Deriving Functional Endothelial Progenitmentioning

confidence: 99%

Efficient Method of Deriving Functional Endothelial Progenitor Cells From Hematopoietic Stem Cells

Shetty¹,

Shah²,

Viswanathan³

2015

Journal of Hematology and Blood Disorders

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EPCs (Endothelial progenitor cells) are the precursors of endothelial cells that form the inner lining of blood vessels and are involved in neovascularization and re-endothelializaton of injured tissues. These cells have the potential for treating a multitude of angiogenic disorders giving a new hope in the arena of vascular biology. In the present study, we have demonstrated that cord blood derived expanded CD34 + cells can be differentiated into functional EPCs in vitro. Differentiation was performed using three different media conditions: media with a combination of various cytokines, media devoid of cytokines and a commercial medium. The differentiated cells were characterized based on their morphology as well as at the molecular and cellular level using RT-PCR, FACS and IC. Functionality of the differentiated cells was also analyzed by various assays such as dye uptake, lectin binding and in vitro tube formation. In brief, the results showed that EPCs cultured in the in-house media devoid of cytokines exhibited both CFU-ECs (Colony forming unitendothelial cells) and ECFCs (Endothelial colony forming cells). These differentiated cells expressed endothelial markers such as CD34, CD105, VEGFR2, VEGFR3, vWF, ecNOS and CD31 at a higher rate as compared to the other media studied, were also functional and secreted growth factors such as VEGF and bFGF. Thus, in this study, we have tried to derive an economically feasible and a scalable approach in successfully differentiating expanded CD34 + cells from cord blood to functional EPCs. However, the safety and efficacy of the differentiated cells needs to be determined in suitable preclinical animal models and further clinical studies.

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Human Endothelial Progenitor Cells

Cited by 345 publications

References 99 publications

MicroRNA-138 inhibits hypoxia-induced proliferation of endothelial progenitor cells via inhibition of HIF-1α-mediated MAPK and AKT signaling

MicroRNA-138 inhibits hypoxia-induced proliferation of endothelial progenitor cells via inhibition of HIF-1α-mediated MAPK and AKT signaling

Human vascular model with defined stimulation medium – a characterization study

Efficient Method of Deriving Functional Endothelial Progenitor Cells From Hematopoietic Stem Cells

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