Purification of eleutherosides by macroporous resin and the active fractions of anti-inflammatory and antioxidant activity from Acanthopanax senticosus extract

Abstract

Eleutherosides are important active ingredients of Acanthopanax senticosus, the method for purification of eleutherosides has attracted much attention. In this article, a method for purification of eleutherosides by macroporous resin and the anti-inflammatory and antioxidant activity of elution fractions from Acanthopanax senticosus extract are investigated. Choosing syringin and eleutheroside E as an indication of eleutherosides, the adsorption characteristics and elution parameters of eleutherosides were investigated from Acanthopanax senticosus extract by various types of macroporous resins. The results show HPD100 provides a much higher adsorption and desorption capacity and the retention rates of syringin and eleutheroside E in the product were 95.43% and 94.96%, respectively. Moreover, the 10-80% ethanol elution fractions were collected from the purification process. We examined the anti-inflammatory activity by evaluating Nitric Oxide (NO) production and the antioxidant activities of eight ethanol elution fractions. As a result, 10-80% ethanol elution fractions showed strong concentration-dependent NO production inhibitory activities and antioxidant activities. The 30-50% ethanol elution fractions are shown to be more active than other fractions for antioxidant and antiinflammatory effects. In addition, no cytotoxicity was observed from the three most potent fractions.

Preparative separation of the flavonoid fractions from Periploca forrestii Schltr. ethanol extracts using macroporous resin combined with HPLC analysis and evaluation of their biological activities

“…The DPPH radicals could be stabilized due to the hydrogen‐donating abilities of the antioxidants . The IC 50 values of the DPPH and ABTS scavenging activities of the six subfractions and the crude ethanol extract are compared in Figure A.…”

Evaluation and Selection of Excellent Provenances of Eleutherococcus senticosus

“…The fruits of the species are rich in various bioactive substances, such as saponins, flavonoids, coumarins, and lignans [3,4]. These substances have important health benefits, including anti-tumor [5], anti-inflammatory [6], neuroprotective [7], cardioprotective [8], antioxidative [9], anti-fatigue [10], antiedema [11] effects, and more. Among these medicinal substances, the most abundant medicinal substances are eleutherosides and flavonoids [12,13].…”

“…Eleutheroside B, also named syringin, is a phenylpropanoid compound. Experimental studies have demonstrated that eleutheroside B possesses excellent pharmacological properties, such as anti-inflammatory effects [6], analgesic and antipyretic effects, sedative and hypnotic effects, and anti-tumor and anti-allergic effects [14]. Apart from its medicinal value, E. senticosus also has significant dietary benefits.…”

Preparative Purification of Liriodendrin fromSargentodoxa cuneataby Macroporous Resin

“…The principle of adsorption is based on electrostatic force, hydrogen bonding interaction, and size sieving action between macroporous resins and different molecular from the solution [ 19 ]. In recent years, macroporous resins are widely used as medium to enrich and separate the secondary metabolites of traditional Chinese medicine and nature plant, including flavonoids [ 20 , 21 ], coumarins [ 22 ], terpenoids [ 23 ], lignans [ 24 ], and phenolics [ 25 , 26 ].…”