Purification of bacteriocin LS1 produced by human oral isolate Lactobacillus salivarius BGHO1

Abstract

Our results imply that LS1 is most probably a new bacteriocin, different from previously described bacteriocins produced by L. salivarius strains. The purification of bacteriocin LS1 enabled the further characterization of LS1 on both the molecular and genetic levels.

Purification and genetic characterisation of the novel bacteriocin LS2 produced by the human oral strain Lactobacillus salivarius BGHO1

“…In addition to its potent antimicrobial activity and human origin, the ability of this strain to survive the harsh conditions present in the gastrointestinal tract makes it a promising candidate for a probiotic. In this study, we describe the identification and genetic characterisation of the novel bacteriocin LS2 isolated from the strain Lactobacillus salivarius BGHO1, which was previously shown to produce bacteriocin LS1 [15]. To our knowledge, this is the first published report on a strain of Lactobacillus salivarius that produces multiple bacteriocins.…”

“…A difference in the antimicrobial spectrum was observed between the cell-free supernatant of the BGHO1 culture and its cell extract. The supernatant, previously characterised to produce the bacteriocin LS1 [15], inhibited the strain Lactobacillus paracasei subsp. paracasei BGBUK2-8, unlike the cell extract that lacked this activity.…”

“…Strain BGHO1 was shown to produce bacteriocin LS1 [15]. Several bacteriocins of Lacrobacillus salivarius strains of different origin have been characterised thus far but, to our knowledge, none of these strains was shown to produce more than one bacteriocin [16][17][18][19].…”

Isolation and characterization of Lactobacillus salivarius MTC 1026 as a potential probiotic

“…In fact, fi nding L. salivarius in human feces is not surprising but the important point is discovering its probiotic property. L. salivarius is found growing naturally in the oral cavities (Busarcevic et al, 2008;Koll et al, 2008), intestines (Ryan et al, 2008), vagina (Juarez et al, 2002;Kilic et al, 2005;Vera et al, 2009), and feces of animals (Nemcova et al, 1997;Robredo and Torres, 2000) such as calves, chickens, pigs (Lahteinen et al, 2010;Yun et al, 2009) and humans (Maldonado et al, 2009). In addition, it was also found in the breast milk of mothers (Maldonado et al, 2010;Martin et al, 2006).…”

Identification of lactic acid bacteria with bio-preservative potential isolated from contaminated avian blood obtained at the slaughterhouse

“…Encoding genes were almost studied for enterocins (Cintas et al 1998, Du Toit et al 2000 but the case of salivaricin is more complicated because there is not enough information about bacteriocin types and the genes envolved in their expression. Although production of bacteriocins by L. salivarius has been previously reported (Pilasombut et al 2006, Busarcevic et al 2008 the mode of action of these antimicrobial peptides has not totally elucidated (Pingitore et al 2009).…”