Isolation and characterization of Lactobacillus salivarius MTC 1026 as a potential probiotic

Tinrat, Sirikhwan; Saraya, Sumarn; Chomnawang, Mullika Traidej

doi:10.2323/jgam.57.365

Cited by 22 publications

(15 citation statements)

References 54 publications

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“…An additional paired-end BLASTn approach, to assign species level 16S rRNA sequences, indicated several important genera were present (Additional file 2: Figure S12) [26]. Lactobacillus reuteri (common broiler gut member, also widely used as probiotic supplement), Lactobacillus salivarius (common swine gut microbiota member used as broiler probiotic that improves production and general health) and Lactobacillus vaginalis (frequently found in broiler gut and a persistent gut coloniser) were the main species within the Lactobacillus genus [27][28][29][30]. Enterococcus genus primarily consisted of species Enterococcus faecium, a widely-used probiotic reported to promote broiler growth and suppress C. jejuni and C. perfringens infections, while stimulating the growth of Lactobacillus and Bifidobacterium [31,32].…”

Section: Specific Microbiota Signatures Identified In Broiler Caecal mentioning

confidence: 99%

Genomic analysis on broiler-associated Clostridium perfringens strains and exploratory caecal microbiome investigation reveals key factors linked to poultry necrotic enteritis

et al. 2019

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Background: Clostridium perfringens is a key pathogen in poultry-associated necrotic enteritis (NE). To date there are limited Whole Genome Sequencing based studies describing broiler-associated C. perfringens in healthy and diseased birds. Moreover, changes in the caecal microbiome during NE is currently not well characterised. Thus, the aim of this present study was to investigate C. perfringens virulence factors linked to health and diseased chickens, including identifying putative caecal microbiota signatures associated with NE. Results: We analysed 88 broiler chicken C. perfringens genomes (representing 66 publicly available genomes and 22 newly sequenced genomes) using different phylogenomics approaches and identified a potential hypervirulent and globally-distributed clone spanning 20-year time-frame (1993-2013). These isolates harbored a greater number of virulence genes (including toxin and collagen adhesin genes) when compared to other isolates. Further genomic analysis indicated exclusive and overabundant presence of important NE-linked toxin genes including netB and tpeL in NE-associated broiler isolates. Secondary virulence genes including pfoA, cpb2, and collagen adhesin genes cna, cnaA and cnaD were also enriched in the NE-linked C. perfringens genomes. Moreover, an environmental isolate obtained from farm animal feeds was found to encode netB, suggesting potential reservoirs of NetB-positive C. perfringens strains (toxinotype G). We also analysed caecal samples from a small subset of 11 diseased and healthy broilers for exploratory microbiome investigation using 16S rRNA amplicon sequencing, which indicated a significant and positive correlation in genus Clostridium within the wider microbiota of those broilers diagnosed with NE, alongside reductions in beneficial microbiota members. Conclusions: These data indicate a positive association of virulence genes including netB, pfoA, cpb2, tpeL and cna variants linked to NE-linked isolates. Potential global dissemination of specific hypervirulent lineage, coupled with distinctive microbiome profiles, highlights the need for further investigations, which will require a large worldwide sample collection from healthy and NE-associated birds.

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Section: Specific Microbiota Signatures Identified In Broiler Caecal mentioning

confidence: 99%

Genomic analysis on broiler-associated Clostridium perfringens strains and exploratory caecal microbiome investigation reveals key factors linked to poultry necrotic enteritis

et al. 2019

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“…Las colonias seleccionadas se expusieron a SB en placas de microtitulación (BD Primaria TM ) con capacidad de 3.5 ml por pozo. Se utilizó una placa por cada concentración y previo al establecimiento de la prueba, se prepararon dos matraces con 100 ml caldo MRS; uno con 0.3 g y otro con 1.5 g de SB de bovino (Oxgall Difco TM ) (17,18) . En la prueba se incluyeron dos testigos negativos: solución MRS-con SB sin bacteria y un MRS-sin SB con bacteria.…”

Section: Resistencia a La Exposición De Sales Biliares En Placas De Munclassified

Isolation and identification of potentially probiotic lactic acid bacteria for Holstein calves in the Mexican Plateau

Landa-Salgado

Caballero-Cervantes

Ramírez-Bribiesca

et al. 2019

Rev. Mex. Cienc. Pecu.

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Los becerros neonatos son expuestos continuamente a un amplio rango de microorganismos habitantes del ambiente, y a patógenos causantes de diarrea. El objetivo de este estudio fue aislar e identificar bacterias acido lácticas (BAL) de la mucosa oral de becerros, calostro y leche de vacas Holstein. El aislamiento de BAL se realizó en caldo y placas con medio ManRogosa Sharp (MRS). Una vez purificadas las colonias bacterianas, se realizaron pruebas morfológicas y bioquímicas para la caracterización de BAL. Se aislaron 16 colonias bacterianas, de las cuales se clasificaron en 12 de la mucosa oral, 2 en leche y 2 en calostro. Estas colonias se evaluaron a pH ácido (4.0 y 4.5) y sales biliares (SB: 0.3 y 1.5 g). Posteriormente se identificaron con el Sistema API50CHL. Las especies aisladas con resistencia a pH de 4 y 1.5 de SB fueron: Leuconostoc mesenteroides, Pediococcus pentosaceus, Lactobacillus plantarum. Lactobacillus crispatus y Lactococcus lactis. Estas colonias cuentan con un potencial probiótico para ser usadas en becerros.

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“…However, in the development of a probiotic product, also the safety, functional and technological aspects need to be considered (Hütt et al 2006;Belicová et al 2013). Probiotic has to withstand harsh gastrointestinal tract conditions (such as low pH, and presence of bile salts) and be able to adhere to intestinal epithelial cells (Kos et al 2003;Kaushik et al 2009;Tinrat et al 2011).…”

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confidence: 99%

Anti-Salmonella Potential of New Lactobacillus Strains with the Application in the Poultry Industry

Kowalska¹,

Nowak

Śliżewska

et al. 2020

Polish Journal of Microbiology

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Probiotics are considered an alternative to antibiotics in the prevention and treatment of Salmonella diseases in poultry. However, to use probiotics as proposed above, it is necessary to evaluate their properties in detail and to select the most effective bacterial strains in the application targeted. In this study, probiotic properties of new Lactobacillus sp. strains were investigated and their antimicrobial activity against 125 environmental strains of Salmonella sp. was determined using the agar slab method. Furthermore, their survival in the presence of bile salts and at low pH, antibiotics susceptibility, aggregation and coaggregation ability, adherence to polystyrene and Caco-2 cells, and cytotoxicity were investigated. Each strain tested showed antagonistic activity against at least 96% of the environmental Salmonella sp. strains and thus representing a highly epidemiologically differentiated collection of poultry isolates. In addition, the probiotic properties of new Lactobacillus strains are promising. Therefore, all strains examined showed a high potential for use in poultry against salmonellosis.

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Isolation and characterization of Lactobacillus salivarius MTC 1026 as a potential probiotic

Cited by 22 publications

References 54 publications

Genomic analysis on broiler-associated Clostridium perfringens strains and exploratory caecal microbiome investigation reveals key factors linked to poultry necrotic enteritis

Genomic analysis on broiler-associated Clostridium perfringens strains and exploratory caecal microbiome investigation reveals key factors linked to poultry necrotic enteritis

Isolation and identification of potentially probiotic lactic acid bacteria for Holstein calves in the Mexican Plateau

Anti-Salmonella Potential of New Lactobacillus Strains with the Application in the Poultry Industry

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