Purification of a thermostable antinociceptive lectin isolated from Andira anthelmia

Abstract

Andira anthelmia (tribe Dalbergieae), a plant from Brazilian Amazon, possesses a seed lectin that was purified by affinity chromatography in sepharose-mannose. This novel Dalbergieae lectin, named AAL, agglutinated rabbit erythrocytes treated with trypsin. The hemagglutinating activity of AAL was maintained after incubation at a wide range of temperature (40 to 70 °C) and pH, was shown to be dependent on divalent cations, and was inhibited by d-mannose and d-sucrose. AAL showed an electrophoretic profile in sodium dodecyl sulfate-polyacrylamide gel electrophoresis similar to other lectins of the tribe Dalbergieae, presenting a double band of molecular weight with approximately 20 kDa and other minor bands of 17, 15, and 13 kDa, being the smaller fragment glycosylated. AAL injected by intravenous route in mice showed antinociceptive activity in two behavioral tests (writhing and formalin). In the writhing test induced by acetic acid, AAL showed inhibitory effect at 0.01 mg/kg (68%), 0.1 mg/kg (46%) and 1 mg/kg (74%). In the formalin test, AAL (0.1 mg/kg) inhibited by 48% the licking time in the inflammatory phase, an effect that was recovered by the lectin association with mannose. In conclusion, AAL presents analgesic effect involving the lectin domain via peripheral mechanisms of inflammatory nociception. This activity highlights the importance of lectins as tools to be used for understanding the interaction of protein-carbohydrate in processes associated to inflammatory pain. Copyright © 2015 John Wiley & Sons, Ltd.

The anti-inflamatory effect of Andira anthelmia lectin in rats involves inhibition of the prostanoid pathway, TNF-α and lectin domain

“…This study demonstrated the modulator effect of the puri ed Andira anthelmia lectin (AAL) on vascular and cellular events of acute in ammation in rats, that was shown to be dependent on the lectin domain. These results corroborate the AAL antinociceptive effect previously in the in ammatory phase of the formalin test [8].…”

“…Andira anthelmia lectin (AAL) was isolated by sepharose-mannose a nity chromatography, presenting binding a nity for mannose and glucose [8]. Animals received AAL (0.01; 0.1; 1 mg/kg) or 0.9% NaCl (sterile saline) by intravenous (i.v.)…”

“…The AAL hemagglutinating activity was inhibited by D-mannose and Dglucose and was shown to be dependent on divalent cations. AAL presents antinociceptive effect in behavioral tests (writhing and formalin), involving the lectin domain and peripheral mechanisms of in ammatory nociception [8]. However, up to date, there is no report in the scienti c literature showing the anti-in ammatory effect of a lectin mannose/glucose-binding belonging to Dalbergieae tribe.…”

Inhibitory effect of Lonchocarpus araripensis lectin in rat acute models of inflammation

“…On the other hand, those possessing binding affinity for N-acetylglucosamine present systemic anti-inflammatory effect, such as the lectins of Lonchocarpus sericeus (Alencar et al 2005, Napimoga et al 2007 and Lonchocarpus araripensis (Pires et al 2016). In addition, Dalbergiae lectins possessing binding affinity for mannose present pro-inflammatory and nociceptive effects, such as the lectins of Centrolobium tomentosum (Almeida et al 2016) and Platypodium elegans (Araripe et al 2017, Cavada et al 2018, except for the lectin of Andira anthelmia, which presents antinociceptive effect (Nascimento et al 2016).…”

Vatairea guianensis lectin stimulates changes in gene expression and release of TNF‐α from rat peritoneal macrophages via glycoconjugate binding

“…9,10 One exception is Andira anthelmia lectin since it demonstrated an inhibitory effect on the inflammatory phase of the formalin test. 11 Other Dalbergieae lectins that possess N-acetyl-D-glucosamine affinity present anti-inflamma tory properties, such as those of Lonchocarpus sericeus, 12,13 Lonchocarpus araripensis, 14,15 and Lonchocarpus campestris. 16 The galactosebinding lectins show proinflammatory effects, such as that elicited by Vatairea macrocarpa [17][18][19] and Vatairea guianensis.…”