Purification, molecular cloning, and properties of a β-glycosidase isolated from midgut lumen of Tenebrio molitor (Coleoptera) larvae

The last piece in the cellulase puzzle: the characterisation of β-glucosidase from the herbivorous gecarcinid land crab Gecarcoidea natalis

“…Transcellobiosylation reactions are a feature of fungal exocellulases (Hayashi et al, 2003;Okada and Nisizawa, 1975). A similar transient ligation step has been observed for -glucosidase from P. cribrata and the mealworm Tenebrio molitor (Ferreira et al, 2001;Scrivener and Slaytor, 1994). Transglycosylation reactions are typical of glucohydrolases; it is believed that such reactions play a role in activating the enzyme through facilitating the production of certain oligomeric substrates (Yernool et al, 2000), as well as in the induction of cellulase gene expression (Mach et al, 1995).…”

“…Cellobiohydrolases appear to play no role in the initial digestion of cellulose by termites and wood-eating cockroaches (Watanabe and Tokuda, 2010); cellulolytic systems from these insects consist of multiple endo--1,4-glucanases and -glucosidases (Genta et al, 2003;Schulz et al, 1986;Scrivener and Slaytor, 1994;Slaytor, 1992;Tokuda et al, 1999). Other invertebrate endo--1,4-glucanases are known to produce cellobiose, cellotriose and cellotetraose (Watanabe and Tokuda, 2010;Xu and Distel, 2004), whereas partially purified -glucosidases from other species are able to hydrolyse cellotriose, cellotetraose and cellopentaose (Ferreira et al, 2001;Ferreira et al, 2003;Marana et al, 2000). It is possible that the cellulase system from these animals employs a similar mechanism to that of G. natalis.…”

“…Like fungal systems, endo--1,4-glucanases and -glucosidases appear to be key components of the cellulase systems of many marine and terrestrial invertebrates (Chararas et al, 1983;Davison and Blaxter, 2005;Ferreira et al, 2001;Linton and Greenaway, 2007;Linton et al, 2006;Pontoh and Low, 2002;Sakamoto et al, 2009;Tokuda et al, 2002;Watanabe and Tokuda, 2010). However, it appears that cellobiohydrolase activity is uncommon among invertebrates (Bärlocher, 1982;McGrath and Matthews, 2000;Sakamoto et al, 2007;Watanabe and Tokuda, 2010), suggesting that many species are capable of producing glucose from crystalline cellulose without requiring a cellobiohydrolase (Scrivener and Slaytor, 1994;Slaytor, 1992;Watanabe and Tokuda, 2010).…”

Degradation of Plant-derived Carbohydrates in Wetlands

“…In 2002, endogenous bglucosidase was found in some kinds of termites such as Neotermes koshunensis (Tokuda et al 2002). Endogenous cellulases were also found in other insects including Lepidoptera Spodoptera frugiperda and Bombyx mori, Coleoptera Tenebrio molitor and Blattodea Leucophaea maderae Ferreira et al 2001;Cornette et al 2003;Byeon et al 2005). Biochemical detection of endoglucanase has been shown in the Mollusca including mussel Mytilus edulis, abalone Haliotis discus hannai, fresh water snail Ampullaria crossean and the clam Corbicula japonica (Xu et al 2001;Suzuki et al 2003;Wang et al 2003;Sakamoto et al 2007;Guo et al 2008;Rahman et al 2014).…”

β-glicosidases intestinais da larva de Diatraea saccharalis: clonagem e sequenciamento dos cDNAs, expressão e algumas propriedades

“…A especificidade das β-glicosidases pode variar, com algumas enzimas sendo capazes de utilizar uma gama enorme de substratos e outras utilizando poucos tipos de compostos (ver Ferreira et al, 1998;Ferreira et al, 2001Ferreira et al, ,2003Azevedo et al, 2003). ; Spodoptera frugiperda, um Lepidoptera com duas β-glicosidases diferentes (Marana et al, 2000) e Diatraea saccharalis, outro Lepidóptera, possui três diferentes β-glicosidases no seu tubo digestivo (Azevedo et al, 2003).…”