Purification, isolation, and structure characterization of water soluble and insoluble polysaccharides from Maitake fruiting body

Yiasmin, Mst Nushrat; Islam, Serajul; He, He; Liu, Yaxian; Wang, Mingming; Yang, Ruijin; Xiao, Hua

doi:10.1016/j.ijbiomac.2020.08.037

Cited by 11 publications

(4 citation statements)

References 24 publications

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“…Reactive oxygen species (ROS) are by-products of normal cellular metabolism [32]. Antioxidants are valuable for their ability to donate electrons, thereby neutralizing radical production and mitigating further damage through free-radical mechanisms [33]. While ascorbic acid is renowned for its antioxidative properties, improper clinical administration may lead to adverse effects such as excessive gastric acid, urinary calculi, and gastrointestinal reactions.…”

Section: Detection Of the Oh − Scavenging Activity Of Rosa Roxburghii...mentioning

confidence: 99%

Inhibition of Proliferation and Induction of Apoptosis in Prostatic Carcinoma DU145 Cells by Polysaccharides from Yunnan Rosa roxburghii Tratt

Yang,

Chen

2024

Molecules

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Objective: This study aimed to investigate methodologies for the extraction and purification of polysaccharides from Rosa roxburghii Tratt fruits and their impact on various cellular processes in prostate cancer DU145 cells, including survival rate, migration, invasion, cell cycle, and apoptosis. Results: Compared to the control group, the polysaccharide exhibited a significant reduction in the viability, migration, and invasion rates of DU145 cells in a time- and dose-dependent manner within the polysaccharide-treated groups. Additionally, it effectively arrested the cell cycle of DU145 cells at the G0/G1 phase by downregulating the expressions of CDK-4, CDK-6, and Cyclin D1. Furthermore, it induced apoptosis by upregulating the expressions of Caspase 3, Caspase 8, Caspase 9, and BAX. Methods: Polysaccharides were extracted from Rosa roxburghii Tratt sourced from Yunnan, China. Extraction and decolorization methods were optimized using response surface methodology, based on a single-factor experiment. Polysaccharide purification was carried out using DEAE-52 cellulose and Sephadex G-100 column chromatography. The optimal dosage of R. roxburghii Tratt polysaccharide affecting DU145 cells was determined using the CCK-8 assay. Cell migration and invasion were assessed using transwell and scratch assays. Flow cytometry was employed to analyze the effects on the cell cycle and apoptosis. Western blotting and Quantitative real-time PCR were utilized to examine protein and mRNA expressions in DU145 cells, respectively. Conclusions: Rosa roxburghii Tratt polysaccharides, consisting of D-mannose, L-rhamnose, N-acetyl-D-glucosamine, D-galacturonic acid, D-glucose, D-galactcose, D-xylose, L-arabinose, and L-fucose, possess the ability to hinder DU145 cell proliferation, migration, and invasion while inducing apoptosis through the modulation of relevant protein and gene expressions.

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Section: Detection Of the Oh − Scavenging Activity Of Rosa Roxburghii...mentioning

confidence: 99%

Inhibition of Proliferation and Induction of Apoptosis in Prostatic Carcinoma DU145 Cells by Polysaccharides from Yunnan Rosa roxburghii Tratt

Yang,

Chen

2024

Molecules

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“…The freeze-dried RPTE was dissolved in distilled water to achieve a concentration of 50 mg/mL, and then filtered through a 0.45 µm membrane. Subsequently, the solution was applied onto a Sephadex G-25 gel filtration column (1.6 × 50 cm) (Ruji Technology Development Co., Ltd., Shanghai, China), which had been pre-equilibrated with distilled water [27]. Elution was carried out using distilled water at a flow rate of 2 mL/min, while monitoring the eluate at 280 nm with a UV-spectrophotometer (Huxi Analysis Instrument Factory Co., Shanghai, China).…”

Section: Sephadex Gel Filtration Fractionationmentioning

confidence: 99%

Unlocking the Therapeutic Potential of a Manila Clam-Derived Antioxidant Peptide: Insights into Mechanisms of Action and Cytoprotective Effects against Oxidative Stress

Jin,

Zhao,

Shi

et al. 2024

Foods

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Reactive oxygen species (ROS) are implicated in various pathological conditions due to their ability to induce oxidative damage to cellular components. In this study, we investigated the antioxidant properties of a peptide isolated from the hydrolysate of Manila clam (Ruditapes philippinarum) muscle. Purification steps yielded RPTE2-2-4, exhibiting potent scavenging activities against DPPH•, HO•, and O2•−, akin to Vitamin C. Structural analysis showed that the isolated peptide, LFKKNLLTL, exhibited characteristics associated with antioxidant activity, including a short peptide length and the presence of aromatic and hydrophobic amino acid residues. Moreover, our study demonstrated the cytoprotective effects of the peptide against H2O2-induced oxidative stress in HepG2 cells. Pretreatment with the peptide resulted in a dose-dependent reduction in intracellular ROS levels and elevation of glutathione (GSH) levels, indicating its ability to modulate cellular defense mechanisms against oxidative damage. Furthermore, the peptide stimulated the expression of the cytoprotective enzyme heme oxygenase-1 (HO-1), further reinforcing its antioxidant properties. Overall, our findings highlight the potential of the Manila clam-derived peptide as a natural antioxidant agent with therapeutic implications for oxidative stress-related diseases. Further investigation into its mechanisms of action and in vivo efficacy is warranted to validate its therapeutic potential.

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“…Therefore, the fructose methylation method was used in the determination according to the literature. 22 Briefly, 2 mg of PCP1 was dissolved in 500 μL of DMSO; to this solution, 1 mg of solid NaOH was added, and the mixture was incubated for 30 min. Thereafter, 50 μL of methyl iodide solution was added and reacted for 1 h. The solution was extracted using 500 μL of methylene chloride, and the sample was evaporated until dry.…”

Section: Structural Characterization Of Pcp1mentioning

confidence: 99%

Structural elucidation and anti-nonalcoholic fatty liver disease activity of Polygonatum cyrtonema Hua polysaccharide

et al. 2022

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Purification, isolation, and structure characterization of water soluble and insoluble polysaccharides from Maitake fruiting body

Cited by 11 publications

References 24 publications

Inhibition of Proliferation and Induction of Apoptosis in Prostatic Carcinoma DU145 Cells by Polysaccharides from Yunnan Rosa roxburghii Tratt

Inhibition of Proliferation and Induction of Apoptosis in Prostatic Carcinoma DU145 Cells by Polysaccharides from Yunnan Rosa roxburghii Tratt

Unlocking the Therapeutic Potential of a Manila Clam-Derived Antioxidant Peptide: Insights into Mechanisms of Action and Cytoprotective Effects against Oxidative Stress

Structural elucidation and anti-nonalcoholic fatty liver disease activity of Polygonatum cyrtonema Hua polysaccharide

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