Purification, in vitro antioxidant and in vivo anti-aging activities of soluble polysaccharides by enzyme-assisted extraction from Agaricus bisporus

Li, Shangshang; Liu, Min; Zhang, Chen; Tian, Chengye; Wang, Xiuxiu; Song, Xinling; Jing, Huijuan; Gao, Zheng; Ren, Zhenzhen; Liu, Weiru; Zhang, Jianjun; Liu, Jia

doi:10.1016/j.ijbiomac.2017.12.108

Cited by 41 publications

(24 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, vitalities of antioxidant enzymes, such as GSH-Px, CAT, and SOD in the liver and kidney of model mice were reduced, while the opposite was true for the MDA level. These results are in line with previous studies [ 6 , 7 , 22 , 23 , 24 ], indicating that the d -gal-induced aging model in mice was successfully established in our present study.…”

Section: Discussionsupporting

confidence: 93%

“…Previous studies have shown that antioxidative enzymes can protect the liver and kidney from d -gal-induced subacute aging by scavenging ROS. Hence, the activities of SOD, CAT, and GSH-Px, as well as the content of malondialdehyde (MDA), were investigated in our present study [ 5 , 6 , 7 ]. As illustrated in Figure 5 , after subcutaneous injection of d -gal for eight weeks, the activities of these three key antioxidative enzymes were sharply decreased in comparison with those in liver and kidney tissues of normal group mice, while the opposite was true for the MDA level (all p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

“…It is well-accepted that the most significant determinant of aging is oxidative damage caused by overproduction of reactive oxygen species (ROS). The formation of excess ROS directly results in oxidative stress and pro-inflammatory responses, which then causes redox disequilibrium, ultimately leading to protein oxidation, lipid peroxidation, and mitochondria and DNA damage, which can precisely inhibit normal cell functions, and even cause apoptosis [ 5 , 6 ]. Meanwhile, ROS is expected to play an additional role in aging by directly or indirectly damaging the endogenous antioxidant system, including superoxide dismutases (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, numerous natural compounds have been discovered to possess potent anti-aging activity and have attracted considerable interest as potential candidates for the development of novel anti-aging therapies [ 6 , 7 , 9 ]. Consequently, close attention has been paid to natural products due to their amicable safety profile and potentially beneficial biological activities, thus, searching natural ingredients with anti-oxidant, immunomodulatory and anti-inflammatory properties for preventing and/or treating this subacute aging has become a new hot spot.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Anti-Aging Effect of Chitosan Oligosaccharide on d-Galactose-Induced Subacute Aging in Mice

Kong

et al. 2018

Marine Drugs

View full text Add to dashboard Cite

Chitosan oligosaccharide (COS), a natural polysaccharide with good antioxidant and anti-inflammatory properties, is the depolymerized product of chitosan possessing various biological activities. The present study was designed to investigate the possible anti-aging effect of COS on the aging model mouse induced by d-galactose (d-gal) and explore the underlying mechanism. In the experiment, 48 male Kunming mice (KM mice) were randomly divided into the normal group, model group, positive group, and low-medium-high dose polysaccharide groups (300, 600, 1200 mg/kg/day). The results showed that COS, by intragastric gavage after subcutaneous injection of d-gal (250 mg/kg/day) into the neck of mice consecutively for eight weeks, gradually recovered the body weight, the activity of daily living, and organ indices of mice, as well as effectively ameliorated the histological deterioration of the liver and kidney in mice triggered by d-gal. To be specific, COS obviously improved the activities of antioxidant enzymes in liver and kidney of KM mice, including catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD), as well as decreased malondialdehyde (MDA) levels when compared with those in model group mice. Furthermore, COS not only elevated the diminished levels of serum immunoglobulin G (IgG) and IgM induced by d-gal, but also significantly inhibited the d-gal-caused upregulation of serum alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), uric acid (UA) and creatinine (CREA) levels as compared with those of mice in the model group. These results demonstrate that COS has an obvious anti-aging activity in d-gal-induced subacute aging mice, the mechanism of which, to some extent, is associated with enhancing the antioxidant defenses, reducing oxidative stress, and improving the immune function of aging model mice.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Anti-Aging Effect of Chitosan Oligosaccharide on d-Galactose-Induced Subacute Aging in Mice

Kong

et al. 2018

Marine Drugs

View full text Add to dashboard Cite

show abstract

“…With the trend of global aging and the improvement of living standards, health problems caused by aging and aging-associated diseases have become increasingly prominent [2,3]. It is well accepted that the most significant determinant of aging is oxidative damage caused by overproduction of reactive oxygen species (ROS) [4][5][6]. Meanwhile, ROS is expected to play an additional role in aging by directly or indirectly damaging the glutathione peroxidase (GSH-Px) and other antioxidase [7].…”

Section: Introductionmentioning

confidence: 99%

Design, synthesis, and preliminary biological evaluation of catalpol propionates as antiaging drugs

et al. 2019

View full text Add to dashboard Cite

In this paper, catalpol propionylated analogs (CPs) were designed as drug ligands of glutathione peroxidase (GSH-Px) based on molecular docking (MD) using Surflex-Docking method. The calculated total scores (Total_score) and C log P of CPs are higher than that of catalpol, which show that the CPs maybe served as potential lead compounds as new antiaging drugs. Furthermore, the maximum Total_score of isomers in one group CPs is often not that the molecule with minimum energy structure. These show that the CPs docking with GSH-Px maybe not only affected by the molecular energy, but also affected by their conformations. The CPs were synthesized by esterification of catalpol with propionic anhydride using pyridine as solvent and acid banding agent, DMAP as catalyst, reaction at specific temperature. The synthesized perpropionylated catalpol analog (CP-6) was determined by NMR, FT-IR, HRMS, and HPLC, and the synthesis process was optimized by means of orthogonal experimental design. Subsequently, CP-6 was screened for cells viability by MTT assay, the results show that the CP-6 can effectively reversed STZ-induced reduction of cells viability, and CP-6 has potential antiaging activity.

show abstract

Research Progress on Biological Activities of Polysaccharides from Edible Fungus

et al. 2023

View full text Add to dashboard Cite

Edible fungus is widely eaten because of their delicious taste and high nutritional value. Growing evidence indicates that edible fungus is rich in various active ingredients including polysaccharides, proteins, fats, vitamins, and other ingredients. Polysaccharides are one of the main active ingredients of edible fungi. Increasing research has confirmed that edible fungus polysaccharides (EFPs) show multiple biological activities such as antioxidant, anti-inflammatory, anti-tumor, immune regulation, anti-virus, gut microbiota regulation, hypoglycemic, etc. Hence, EFP related research has received more and more attention due to their non-toxic, high medical, and nutritional values. This paper reviews the latest research progress of EFPs biological activities, and looks forward to their future development trend. This review provides a theoretical basis for studying the application of EFPs in the fields of biomedicine, cosmetics, health food, and promotes the development and utilization of EFPs.

show abstract

Purification, in vitro antioxidant and in vivo anti-aging activities of soluble polysaccharides by enzyme-assisted extraction from Agaricus bisporus

Cited by 41 publications

References 45 publications

Anti-Aging Effect of Chitosan Oligosaccharide on d-Galactose-Induced Subacute Aging in Mice

Anti-Aging Effect of Chitosan Oligosaccharide on d-Galactose-Induced Subacute Aging in Mice

Design, synthesis, and preliminary biological evaluation of catalpol propionates as antiaging drugs

Research Progress on Biological Activities of Polysaccharides from Edible Fungus

Contact Info

Product

Resources

About