Anti-Aging Effect of Chitosan Oligosaccharide on d-Galactose-Induced Subacute Aging in Mice

Abstract

Chitosan oligosaccharide (COS), a natural polysaccharide with good antioxidant and anti-inflammatory properties, is the depolymerized product of chitosan possessing various biological activities. The present study was designed to investigate the possible anti-aging effect of COS on the aging model mouse induced by d-galactose (d-gal) and explore the underlying mechanism. In the experiment, 48 male Kunming mice (KM mice) were randomly divided into the normal group, model group, positive group, and low-medium-high dose polysaccharide groups (300, 600, 1200 mg/kg/day). The results showed that COS, by intragastric gavage after subcutaneous injection of d-gal (250 mg/kg/day) into the neck of mice consecutively for eight weeks, gradually recovered the body weight, the activity of daily living, and organ indices of mice, as well as effectively ameliorated the histological deterioration of the liver and kidney in mice triggered by d-gal. To be specific, COS obviously improved the activities of antioxidant enzymes in liver and kidney of KM mice, including catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD), as well as decreased malondialdehyde (MDA) levels when compared with those in model group mice. Furthermore, COS not only elevated the diminished levels of serum immunoglobulin G (IgG) and IgM induced by d-gal, but also significantly inhibited the d-gal-caused upregulation of serum alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), uric acid (UA) and creatinine (CREA) levels as compared with those of mice in the model group. These results demonstrate that COS has an obvious anti-aging activity in d-gal-induced subacute aging mice, the mechanism of which, to some extent, is associated with enhancing the antioxidant defenses, reducing oxidative stress, and improving the immune function of aging model mice.

Lactobacillus paracasei ssp. paracasei YBJ01 reduced d-galactose–induced oxidation in male Kuming mice

“…After the oxidative senescence of the body, a weak body could lead to weight loss (Kong et al, 2018). This was seen our study, as mice in the model group showed lower BW than that of mice in the normal group, and LPSP-YBJ01 could inhibit BW loss.…”

Chitosan oligosaccharide as an effective feed additive to maintain growth performance, meat quality, muscle glycolytic metabolism, and oxidative status in yellow-feather broilers under heat stress

“…(2011) reported that COS protected the adverse effects of lipopolysaccharide on the liver of rats by decreasing the serum ALT and AST level. Kong et al. (2018) also reported that COS inhibited the D-galactose–induced upregulation of the serum ALT and AST level.…”

<p>Regulatory Effect of Anwulignan on the Immune Function Through Its Antioxidation and Anti-Apoptosis in D-Galactose-Induced Aging Mice</p>

“…30 D-gal is a reducing sugar, which causes the increase in free radicals and induces the oxidative damage, 31 finally leading to the gradual aging and damage of the body similar to those in natural aging. 32,33 It has been reported that in the aging host, the levels of cytokines also change, and they play an important regulatory role in the immune system. 34,35 In the current study, we found that Anwulignan increased the serum levels of IL-2, IL-4, and IFN-γ in the aging mice, while it decreased the serum levels of IL-6 and TNF-α, suggesting that Anwulignan can restore the immune function that is declined in the process of aging by regulating the level of the cytokines in mice and reversing the inflamm-aging process.…”