Purification, crystallization and partial characterization of the antitumour and insecticidal protein subunit from the σ-endotoxin of Bacillus thuringiensis var. thuringiensis

Prasad, S. Siva; Shethna, Y. I.

doi:10.1016/0304-4165(74)90151-2

Cited by 25 publications

(10 citation statements)

References 17 publications

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“…Bacillus thuringiensis (Bt) synthesizes δ-endotoxins (Cry, Cyt or parasporin proteins) in its parasporal body during sporulation which was reported for antitumor e ects and unique activity speci cally against cancer cells (Mizuki et al, 1999;Prasad & Shethna, 1974) besides long history and application as biopesticide for larvicidal activity. e toxicity of the parasporins were reported against various cancer cells such as MOLT-4, HeLa,…”

Section: Bacillus Thuringiensismentioning

confidence: 99%

Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules

Ferdous¹,

Shishir²,

Khan³

et al. 2018

Microb. Bioacts.

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Cancer treatment remains as an expensive process due to the cost of sophisticated infrastructure development as well as its maintenance with skilled personnel. At the same time, the success rate is not very inspiring since non-specific target oriented medication could cause other health complexities leading to death. Research for alternative therapies aimed at minimizing the side effects of treatments and increasing the survival rates of patients includes routine explorations for anticancer agents from numerous sources (e.g. microbes, plants and nanoparticles). Anticancer activities of several bacterial components especially from Bacillus spp. were reported in many scientific reports. For economic production of these agents, potential strains from this genus could be feasible and sustainable for their long and successful utilization in industries. The review is therefore, focused on describing the available anticancer and anti-proliferative agents reported worldwide from Bacillus spp.

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Section: Bacillus Thuringiensismentioning

confidence: 99%

Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules

Ferdous¹,

Shishir²,

Khan³

et al. 2018

Microb. Bioacts.

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“…The large number of polypeptides with widely ranging molecular weight values (1 x 103 to 7 x 105) that have been reported in the literature (9,10,12,21,25,29) for the solubilized crystal may be the result of subunit autolysis brought about by the traditional alkali treatment (pH 5 10) or aggregation of the highly associating glycoprotein. Our study reveals that the parasporal crystal has the capacity for autolytic activity, apparently involving a sulfhydryl protease that is activated under alkaline conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus thuringiensis produces an intracellular proteinaceous crystal that has insecticidal (3,21,23), antitumor (21), and immune response-enhancing properties (20). In our laboratory, we have been concerned with the first of these properties and are trying to determine the biochemical basis of insect toxicity.…”

mentioning

confidence: 99%

Characterization of the entomocidal parasporal crystal of Bacillus thuringiensis

Bulla¹,

Kramer²,

Davidson³

1977

J Bacteriol

142

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The parasporal crystalline protoxin of Bacillus thuringiensis contains a single glycoprotein subunit that has a molecular weight of approximately 1.2 X 10(5). The carbohydrate consists of glucose (3.8%) and mannose (1.8%). At alkaline pH, the proendotoxin is apparently solubilized and activated by an autolytic mechanism involving an inherent sulfhydryl protease that renders the protoxin insecticidal. Activation generates protons, degraded polypeptides, sulfhydryl group reactivity, proteolytic activity, and insect toxicity. Chemical modification of the sulfhydryl groups inhibits the proteolytic and insecticidal activities, suggesting that cysteine residues may be present in the active site of the protein.

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“…We have also observed that this crystal brings about long-lasting antitumor immunity (21) and enhances the nonspecific immune response (20). Further, the purification, crystallization, and characterization of the biologically active protein subunit of the crystals have been achieved (19).…”

mentioning

confidence: 87%

Mode of Action of a Purified Antitumor Protein from the Proteinaceous Crystal of Bacillus thuringiensis subsp. thuringiensis on Yoshida Ascites Sarcoma Cells

Prasad

Shethna

1976

Antimicrob Agents Chemother

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A purified antitumor protein from the proteinaceous crystal ofBacillus thuringiensis subsp. thuringiensis inhibits the growth of Yoshida ascites sarcoma both in vivo and in vitro. Exogenous respiration of the tumor cells was unaffected by the protein at a concentration as high as 500 Ag/ml. The antitumor protein inhibits the uptake and incorporation of labeled precursors into macromolecules. However, the ratio of incorporation over uptake is not affected by the protein. Further, the protein brings about the leakage of 260-nm-absorbing material, proteins, and 32P-labeled cellular constituents from the Yoshida ascites sarcoma cells. The results show that the action of the antitumor protein appears to alter the cellular permeability of the tumor cells.

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Purification, crystallization and partial characterization of the antitumour and insecticidal protein subunit from the σ-endotoxin of Bacillus thuringiensis var. thuringiensis

Cited by 25 publications

References 17 publications

Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules

Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules

Characterization of the entomocidal parasporal crystal of Bacillus thuringiensis

Mode of Action of a Purified Antitumor Protein from the Proteinaceous Crystal of Bacillus thuringiensis subsp. thuringiensis on Yoshida Ascites Sarcoma Cells

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