Purification, Characterization, and Potential of Saline Waste Water Remediation of a Polyextremophilicα-Amylase from an Obligate HalophilicAspergillus gracilis

Abstract

An obligate halophilic Aspergillus gracilis which was isolated from a hypersaline man-made saltern from Thailand was screened for its potential of producing extracellular α-amylase in the previous studies. In this study the α-amylase was extracted and purified by the help of column chromatography using Sephadex G-100 column. Presence of amylase was verified by SDS-PAGE analysis, showing a single band of approximately 35 kDa. The specific activity of the enzyme was found to be 131.02 U/mg. The Lineweaver-Burk plot showed the V max and K m values of 8.36 U/mg and 6.33 mg/mL, respectively. The enzyme was found to have the best activity at 5 pH, 60°C, and 30% of NaCl concentration, showing its polyextremophilic nature. The use of various additives did not show much variation in the activity of enzyme, showing its resilience against inhibitors. The enzyme, when tested for its use for synthetic waste water remediation by comparing its activity with commercial amylase in different salt concentrations showed that the α-amylase from A. gracilis was having better performance at increasing salt concentrations than the commercial one. This shows its potential to be applied in saline waste water and other low water activity effluents for bioremediation.

Purification and Characterization of Extracellular, Polyextremophilic α-amylase Obtained from Halophilic Engyodontium album

“…This capability is much greater than the previously reported bacterial and Archeal amylases (6,9,30). Ability of this amylase to work at extreme salt concentrations makes them the best available candidate to work for bioremediation processes, particularly, saline waste management (31,32). Similarly the limiting water resources and availability of hard water for domestic uses in current climate change, requires the additive amylases for detergents to work better in low water activity for minimizing the consumption of water, as well as to make more cleaning effects in the presence of high salt concentrations (33).…”

Functional hydrogels with a multicatalytic activity for bioremediation: Single‐step preparation and characterization

“…Several food, pharmaceutical, and textile industry effluents are rich in starches, proteins, and oil‐rich compounds, thus the digestion of such organic pollutants with hydrolytic enzymes can help in wastewater bioremediation . Here, catalytic hydrogels were developed in the form of polymeric films, bearing three activities because of the covalent immobilization of the enzymes contained in the Pancreatin complex (PAN).…”

Production and identification of two antifungal terpenoids from the Posidonia oceanica epiphytic Ascomycota Mariannaea humicola IG100

“…In the last decades, the interest in marine and marine derived fungi has been growing due to their high e cacy in the synthesis of secondary metabolites [4,5,[10][11][12][13][14][15][16][17][18]. These organisms are also promising sources of enzymes useful in different technological applications [19][20][21]. In this context, halophilic fungi have been applied in the ne and specialty chemical elds, as well as in the production of biodiesel and bioremediation [9,[22][23][24][25][26].…”