Purification, characterization, and bioactivities of a polysaccharide from mycelial fermentation of Bjerkandera fumosa

Liu, Di; Sun, Qianwen; Xu, Jing; Li, Na; Lin, Jianan; Chen, Shan; Fan, Li

doi:10.1016/j.carbpol.2017.03.029

Cited by 57 publications

(21 citation statements)

References 34 publications

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“…GUPS-III appears to be sulfated polysaccharides. GUPS-I might be a neutral polysaccharide because it coeluted with distilled water, whereas both GUPS-II and GUPS-III, which eluted with NaCl solution, might be acidic polysaccharides [29]. The results are similar to previous studies which reported that three major fractions can be obtained from crude polysaccharides of G. uralensis [30,31], but different from another study which reported that five fractions can be observed [46].…”

Section: Discussionsupporting

confidence: 70%

“…As shown in Figure 4, the IR spectra of GUPS-I, GUPS-II, and GUPS-III had a broad, intense hydroxyl group stretching band at 3400-3300 cm −1 and a weak C-H stretching band at around 3000-2800 cm −1 , which are the characteristics of polysaccharides [12,33]. In the three spectra, the absorption peaks at approximately 1610-1655 cm −1 corresponded to carbonyl groups (C=O) and were caused by the bending mode of bound water, while the absorption peak at approximately 1400 cm −1 was the C-O stretching vibration [26,29,34,35]. A strong absorption peak at 1615 cm −1 presented carboxyl groups (COO−) in GUPS-III, indicating the existence of uronic acids [36,37].…”

Section: The Analysis Of Gups By Ft-ir Spectroscopymentioning

confidence: 95%

“…As shown in Figure 1, three fractions, i.e., GUPS-I, GUPS-II, and GUPS-III, were collected, concentrated, dialyzed, and lyophilized. GUPS-I eluted with distilled water might be a neutral polysaccharide, whereas both GUPS-II and GUPS-III eluted with NaCl solution might be acidic polysaccharides [29]. The physicochemical properties of the GUPS were determined and shown in Table 1.…”

Section: Purification and Physicochemical Characterization Of Gupsmentioning

confidence: 99%

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Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

et al. 2020

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Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities. Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively. The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different. The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages. Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I. An in vivo experiment showed that only GUPS-II significantly enhanced the maturation of DCs. These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.

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Section: Discussionsupporting

confidence: 70%

Section: The Analysis Of Gups By Ft-ir Spectroscopymentioning

confidence: 95%

Section: Purification and Physicochemical Characterization Of Gupsmentioning

confidence: 99%

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Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

et al. 2020

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“…The results of the monosaccharide‐composition analysis through GC are shown in Figure . Compared with some polysaccharides which have antioxidant and immunological activity (Kim, Park, Nam, Lee, & Lee, ; Lee et al, ; Liu et al, ), SGP I has a similar molecular mass and SGP II has a lower molecular mass. SGP I‐b comprised four monosaccharides, namely, fucose, mannose, glucose, and galactose at the molar ratios of 1.86:1.61:1:4.14.…”

Section: Resultsmentioning

confidence: 99%

Structural characterization, antioxidant activity, and immunological activity in vitro of polysaccharides from fruiting bodies of Suillus granulatus

Chen

Wang

2018

J Food Biochem

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Two polysaccharide fractions, SGP I-b and SGP II-b, were purified from the fruiting bodies of Suillus granulatus. The assayed relative molecular masses of SGP I-b and SGP II-b were approximately 17.31 and 10.16 kDa, respectively. SGP I-b consisted of fucose, mannose, glucose, and galactose, whereas SGP II-b consisted of rhamnose, fucose, mannose, glucose, and galactose. The structures of SGP I-b and SGP II-b were preliminarily analyzed through methylation analysis and nuclear magnetic resonance spectroscopy. The results of in vitro antioxidant assays revealed that SGP II-b demonstrated good scavenging ability for hydroxyl and DPPH radicals but poor reduction capacity and scavenging for superoxide anions. SGP I-b did not exhibit any significant antioxidant activities. SGP II-b could also significantly increase lymphocyte proliferation in vitro, however, SGP I-b had no significant effect. Practical applicationsSuillus granulatus is a wild, edible medicinal fungus distributed in Northeastern China. S. granulatus polysaccharides (SGP) already be prove have special bioactivities. In the current study, SGP were purified and characterized, and the antioxidant activities and immunomodulatory effects were also assayed. Our work will contribute to the utilization of SGP as natural antioxidants and/or immunity-enhancing agents in food and pharmaceutical industries.

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“…Lymphocyte transformation rate is an imperative indicator to evaluate the cellular immune function [30]. Lymphocyte proliferation is a normal protective physiological response of the body's immune system after stimulation by foreign antigens, which is of great significance for the body's combating against exogenous microbial infection [31]. Our results showed that MLPs could promote the proliferation and transformation of lymphocytes.…”

Section: Discussionmentioning

confidence: 66%

Effects of Dietary Supplementation with Mulberry (Morus alba L.) Leaf Polysaccharides on Immune Parameters of Weanling Pigs

Zhao

Yang

et al. 2019

Animals

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Simple Summary: The increasing overuse/misuse of restricted antibiotics in livestock and poultry production has raised a serious health concern. Thus, aiming to improve animals' health levels, researchers are redirecting their focus onto naturally occurring bioactive ingredients from plants; these compounds have become a potential substitute for antibiotics. Mulberry leaf polysaccharide (MLP) is an important bioactive component, which has notable potential for improving animal immunity. In this context, the present study was designed to investigate the MLPs' effect on animals' metabolisms and the immune parameters of weanling pigs.Abstract: In this study, the effect of dietary supplementation of mulberry leaf polysaccharides (MLPs) on the immune parameters-i.e., the immune organ weight, serum immunoglobulins, cytokines, nitric oxide (NO) production, and insulin-Like growth factor-1 (IGF1) mRNA expression-of weanling pigs as a model animal was investigated. A total of 120 healthy weanling pigs (aged 28 ± 2 d) with the same body weights were randomly divided into four groups: (1) Control treatment (CT), basal diet (BD), (2) MLP low-dose treatment (MLT), 0.6 g/kg MLP + BD, (3) MLP high-dose treatment (MHT), 1.2 g/kg MLP + BD, and (4) antibiotic treatment (AT), 0.15 g/kg chlortetracycline + BD. The results revealed that the thymus and spleen indices were significantly increased (P < 0.05) in both MLT and MHT groups in comparison with the CT group, while the serum levels of immunoglobulin G (IgG), interleukin (IL)-1β, IL-2, IL-8, and interferon (IFN-γ) in the MLT group and IL-2, IL-6, and IFN-γ in the MHT group were also considerably greater (P < 0.05) than the corresponding levels in the CT group. The serum contents of IgG, IL-1β, IL-2, and IL-8 in the MLT group and IL-2 and IL-6 in the MHT group were significantly increased in comparison with the corresponding contents in the AT group (P < 0.05). The transformation rate of lymphocytes in the MLT and MHT groups was higher compared to the CT and AT groups. However, a notable difference was found between the MLT group and the two control groups. The peripheral lymphocyte NO production in the MLT, MHT, and AT groups was significant relative to the CT group. The expression levels of IGF1 mRNA in the liver and muscle longissimus tissues of both the MLT and MHT groups showed significant improvement (P < 0.05) over those in the CT group. Moreover, the IGF1 mRNA expression in the muscle longissimus from the MLT group was significantly higher than in the AT group. In conclusion, the results suggest that incorporating MLPs into the diets of weanling pigs improves the animals' metabolisms and immune functions, and the effects of the MLT group were superior to those of both the MHT and AT groups.

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Purification, characterization, and bioactivities of a polysaccharide from mycelial fermentation of Bjerkandera fumosa

Cited by 57 publications

References 34 publications

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

Structural characterization, antioxidant activity, and immunological activity in vitro of polysaccharides from fruiting bodies of Suillus granulatus

Effects of Dietary Supplementation with Mulberry (Morus alba L.) Leaf Polysaccharides on Immune Parameters of Weanling Pigs

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