Purification, characterization and anti-diabetic activity of a polysaccharide from mulberry leaf

Zhang, Yao; Ren, Chunjiu; Lu, Guobing; Cui, Wei-Zheng; Mu, Zhimei; Gao, Huiju; Wang, Yanwen

doi:10.1016/j.yrtph.2014.10.006

Cited by 67 publications

(30 citation statements)

References 31 publications

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“…The leaves of the mulberry tree are known worldwide as sources of phytotherapeutics, which have been used for the treatment of conditions related to diabetes and been recorded based on clinical trials or the use of animal models (Banu et al, ; El‐Sayyed, Badawy, Elnabi, El‐Elaimy, & Shehari, ; Zhang et al, ). A large number of animal and human studies in vivo suggested that mulberry leaves has the antidiabetic and its complications activity (Andallu & Varadacharyulu, ; Do et al, ; Volpato et al, ; Zhang et al, ). Many different types of constituents in mulberry leaves have been linked to prevention and treatment of diabetes and its complications (Guo et al, ; Li et al, ; MA et al, ).…”

Section: Introductionmentioning

confidence: 99%

The mechanism of mulberry leaves against renal tubular interstitial fibrosis through ERK1/2 signaling pathway was predicted by network pharmacology and validated in human tubular epithelial cells

Zhu

et al. 2019

Phytotherapy Research

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Mulberry leaf was reported that it has antidiabetic activity, although the mechanisms underlying the function have not been fully elucidated. In the present study, the results of network pharmacology suggested that mulberry leaves could regulate key biological process in development of diabetes, and the process implicates multiple signaling pathways, such as JAK-STAT, MAPK, VEGF, PPAR, and Wnt. Then, the research in vitro indicated that mulberry leaves remarkably ameliorated high glucose-induced epithelial to mesenchymal transition, which was characterized with significant reduction of intracellular reactive oxygen species (ROS) levels as well as downregulation of NADPH oxidase subunits NOX1, NOX2, and NOX4, and it was found to be connected with the ERK1/2 signaling pathway in human tubular epithelial cells (HK-2). Moreover, the results of bioinformatics and the dual luciferase report showed that ZEB1 might be a target gene of miR-302a; decreased miR-302a and increased ZEB1 expressions could significantly promote epithelial to mesenchymal transition. However, mulberry leaves could reverse these modulations. Our results demonstrated that network pharmacology could provide a guidance role for traditional Chinese medicine research, and mulberry leaves could be of benefit in preventing high glucose-induced EMT in HK-2 cells, which proved that it was related to the upregulation of miR-302a by targeting ZEB1 and the inhibition of NADPH oxidase/ROS/ERK1/2 pathway.

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Section: Introductionmentioning

confidence: 99%

The mechanism of mulberry leaves against renal tubular interstitial fibrosis through ERK1/2 signaling pathway was predicted by network pharmacology and validated in human tubular epithelial cells

Zhu

et al. 2019

Phytotherapy Research

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“…Oral administration of mulberry leaf at 50-200 mg/ kg body weight daily for 5 weeks significantly reduced the levels of fasting blood glucose, glycosylated serum protein, serum total cholesterol, and serum triglyceride, and increased the body weight, fasting insulin, C-peptide, liver glycogen, liver glucokinase, and serum high-density lipoprotein cholesterol. Moreover, mulberry leaf promoted marked pancreatic b-cell regeneration and insulin secretion, and reduced liver fat accumulation in diabetic rats (47,48).…”

Section: Reducing Blood Sugar and Insulin Resistancementioning

confidence: 93%

Anti-obesity effect of traditional Chinese medicine and natural products

Chen¹,

Lian²,

Lyu³

et al. 2016

Eur J Bio Med Res

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Abstract:As obesity has become a worldwide health problem over the past three decades, more emphasis are placed on the prevention and treatment of obesity.Research in the nutrition field has recently aroused considerable interest based on the potential of traditional Chinese medicine and natural products to counteract obesity. This review focuses on the anti-obesity action usingtraditional Chinese medicine and natural products. Some present potent anti-obesity effects because of their ability to reduce blood lipid or blood glucose while others could be used for the treatment of obesity as lipase inhibitor, lipid metabolism regulators, appetite suppressants or fatty acid synthase inhibitor. Further study should be conducted in the mechanisms and active ingredients of traditional Chinese medicine and natural products.

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“…27 The highly-purified polysaccharides α-arabinose, α-xylose, α-glucose, α-rhamnose, and α-mannose that were extracted from mulberry leaf were able to induce regeneration of pancreatic β-cells, which were then able to effectively secreted insulin and reduce liver fat accumulation in diabetic rats. 28 Glycosides and their aglycons that were isolated from a water extract of mulberry leaf were found to have potential type 2 anti-diabetic activity. 29 While the inhibition of α-glucosidase plays a crucial role in the anti-diabetes effect of mulberry leaf, an individual compound cannot elucidate metabolic mechanisms, thus molecular mechanisms need invetigating.…”

Section: Polysaccharidesmentioning

confidence: 99%