Purification, cDNA cloning, and characterization of plant chitinase with a novel domain combination from lycophyte Selaginella doederleinii

Abstract

AtChiC: Arabidopsis thaliana class V chitinase; CBB: Coomassie brilliant blue R250; CBM: carbohydrate binding module family; CrChi-A: Cycas revolute chitinase-A; EaChiA: Equisetum arvense chitinase-A; GH: glycoside hydrolase family, GlxChi-B: gazyumaru latex chitinase-B; GlcNAc: N-acetylglucosamine; HPLC: high performance liquid chromatography; LysM; lysin motif; MtNFH1: Medicago truncatula ecotypes R108-1 chitinase; NCBI: national center for biotechnology information; NF: nodulation factor; NtChiV: Nicotiana tabacum class V chitinase; PCR: polymerase chain reaction; PrChi-A: Pteris ryukyuensis chitinase-A; RACE: rapid amplification of cDNA ends; SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis; SdChiA: Selaginella doederleinii chitinase-A.

Chitinase-Assisted Bioconversion of Chitinous Waste for Development of Value-Added Chito-Oligosaccharides Products

“…Under the same conditions, the chitin-binding ability of PrChiA is lower than chitinase from Equisetum arvense (EaChiA), but the binding mode of these two chitinases for (GlcNAc) n are comparable [80,81]. The cleavage patterns of (GlcNAc) n produced by chitinase from Selaginella doederleinii (SdChiA) are also similar, except for (GlcNAc) 4 which is mainly hydrolyzed to (GlcNAc) 2 [82].…”

“…The mode of interaction of LysM in the chitinases among plants may differ, for example, LysM acting in chitin-signaling immunity in fern and higher plants, which have been extensively studied. For subtropical plant species, i.e., fern and moss, the recent research reported LysM-containing chitinases from Pteris ryukyuensis [78,79], Equisetum arvense [80,81], Selaginella doederleinii [82], and moss Bryum coronatum [83]. The LysM domains of chitinase PrChiA from Pteris ryukyuensis are demonstrated to bind to chitin and COS. (GlcNAc) 6, (GlcNAc) 5 , and (GlcNAc) 4 are the most frequently hydrolyzed into (GlcNAc) 4 +(GlcNAc) 2, (GlcNAc) 3 +(GlcNAc) 2 , and (GlcNAc) 3 +GlcNAc, respectively.…”