Purification, biochemical characterisation and partial primary structure of a new α-amylase inhibitor from Secale cereale (rye)

Iulek, J.; Franco, Octávio L.; Silva, Márcio; Slivinski, Christiane Trevisan; Bloch, Carlos; Rigden, Daniel J.; Sá, Maria Fátima Grossi de

doi:10.1016/s1357-2725(00)00053-4

Cited by 85 publications

(64 citation statements)

References 47 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…3.2.1.1) is one of the major secretory products of the pancreas (about 5-6%) (16) and salivary glands, playing a role in digestion of starch and glycogen and can be found in microorganisms, plants and higher organisms (17). The α -amylase constitute a family of endoamylases that catalyse the initial hydrolysis of starch into shorter oligosaccharides through the cleavage of α-D-(1-4) glycosidic bonds (17)(18)(19)(20). Neither terminal glucose residues nor α-1,6-linkages can be cleaved by α-amylase (16).…”

Section: Characteristics Of -Amylasementioning

confidence: 99%

See 1 more Smart Citation

α-Amylase Inhibitors: A Review of Raw Material and Isolated Compounds from Plant Source

et al. 2012

View full text Add to dashboard Cite

-Inhibition of α-amylase, enzyme that plays a role in digestion of starch and glycogen, is considered a strategy for the treatment of disorders in carbohydrate uptake, such as diabetes and obesity, as well as, dental caries and periodontal diseases. Plants are an important source of chemical constituents with potential for inhibition of α-amylase and can be used as therapeutic or functional food sources. A review about crude extracts and isolated compounds from plant source that have been tested for α-amylase inhibitory activity has been done. The analysis of the results shows a variety of crude extracts that present α-amylase inhibitory activity and some of them had relevant activity when compared with controls used in the studies. Amongst the phyto-constituents that have been investigated, flavonoids are one of them that demonstrated the highest inhibitory activities with the potential of inhibition related to number of hydroxyl groups in the molecule of the compound. Several phyto-constituents and plant species as α-amylase inhibitors are being reported in this article. Majority of studies have focused on the anti-amylase phenolic compounds.

show abstract

Section: Characteristics Of -Amylasementioning

confidence: 99%

“…The amylase presents a three-dimensional structure capable of binding to substrate and, by the action of highly specific catalytic groups, promote the breakage of the glycoside links (20). The protein contains 3 domains: A, B, and C. Domain A, which has a (β/α) 8 barrel fold, constitutes the catalytic core domain.…”

Section: Characteristics Of -Amylasementioning

confidence: 99%

α-Amylase Inhibitors: A Review of Raw Material and Isolated Compounds from Plant Source

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Numerous studies have demonstrated that cereal grains contain variable numbers of proteins with characteristics of digestive enzyme inhibitors of mammals, insects, and bacteria (1)(2)(3)(4)(5)(6)(7). These proteins for many years were recognized only as undesirable, anti-nutritional substances: they decrease the availability of nutritional components (6)(7)(8)(9)(10)(11) and moreover are listed among protein fractions causing food allergies ( 12,13 ) .…”

mentioning

confidence: 99%

Digestive Enzyme Inhibitors from Grains as Potential Components of Nutraceuticals

Piasecka‐Kwiatkowska

Warchalewski

Zielińska‐Dawidziak

et al. 2012

J Nutr Sci Vitaminol

View full text Add to dashboard Cite

Summary Inhibitor activity against digestive enzymes, such as ␣ -amylase from human saliva and porcine pancreas and trypsin from bovine pancreas, of three cereal grains species were studied as potential components of nutraceuticals strengthening diabetes and obesity treatment. Significant differences were demonstrated: the highest antitryptic activity was found in the grain of the rye varieties studied, whereas the grain of the wheat varieties had significantly higher ability to inhibit ␣ -amylases from human saliva and porcine pancreas. Additionally, seeds of Puma, one of the studied wheat varieties, demonstrated especially low antitryptic activity. Such a beneficial arrangement of inhibitors, i.e. high level of inhibitors of ␣ -amylase from human saliva and porcine pancreas and simultaneously low level of trypsin inhibitors from bovine pancreas, indicate the possibility of the application of seeds with such properties to the preparation of nutraceuticals for people with obesity or suffering from diabetes.

show abstract

“…These proteins have the biological activities towards several insect taxa such as Coleopteran, Lepidopteran and Homopteran (Ussuf et al, 2001;Regnault-Roger et al, 2002Louis, 2004Karimi et al, 2010). In the pea, the entomotoxic protein is called albumin PAb1; there is also a lectin PSA; in bean, the entomotoxic factor is arcelin and lectin PHA (Iulek et al, 2000;Louis, 2004;Silué, 2009;Karimi et al, 2010). Other researchers have shown the anti-insect activities for these and other sources of the flours of leguminous seeds (Carlini and Grossi-de-Sa, 2002;Louis, 2004;Wang et al, 2006;Fatimé, 2007;Tamgno, 2009;Mebarkia et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Bioactivity of flours of seeds of leguminous crops <i>Pisum sativum, Phaseolus vulgaris</i> and <i>Glycine max</i> used as botanical insecticides against Sitophilus oryzae Linnaeus (Coleoptera: Curculionidae) on sorghum grains

Tinkeu¹,

Tamgno²,

Gandebe³

2016

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

Cereals occupy an important place in the solving of food insecurity problems in the northern Cameroon. Chemicals continue to be the main tools to protect cereals during storage in spite of the deleterious effects due to their current usage. Nowadays, entomotoxic proteins of seeds of leguminous are more and more considered as alternative insecticides. Efficacy of crude flours of four legumes seeds, applied to the sorghum grains, was evaluated against Sitophilus oryzae L. (Coleoptrea: Curculionidae) by contact-ingestion. This research revealed that flours of bean and pea are very active on S. oryzae. The lethal dose 100 (LD 100 ) and the lethal times 100 (LT 100 ) are respectively 5 g and 20 days for pea's flour and 10 g and 50 days for bean's flour. The toxicity of flours' seeds increased with the dose and the duration of application (F=169.734***, Df =6; 28). During a period of 90 days, the flours of P. sativum and P. vulgaris keep their insecticidal activities, showing constant mortality rates for bean (F= 2.325ns, Df =6; 28) and for pea (F=1,91ns, df= 34). The flours of seeds of P. sativum and P. vulgaris at the weight ratio of 5 % and 10 % respectively can provide protection of sorghum grains against the attack of S. oryzae. The legumes seeds are the alternative active ingredients to hazardous conventional insecticides.

show abstract

Purification, biochemical characterisation and partial primary structure of a new α-amylase inhibitor from Secale cereale (rye)

Cited by 85 publications

References 47 publications

α-Amylase Inhibitors: A Review of Raw Material and Isolated Compounds from Plant Source

α-Amylase Inhibitors: A Review of Raw Material and Isolated Compounds from Plant Source

Digestive Enzyme Inhibitors from Grains as Potential Components of Nutraceuticals

Bioactivity of flours of seeds of leguminous crops <i>Pisum sativum, Phaseolus vulgaris</i> and <i>Glycine max</i> used as botanical insecticides against Sitophilus oryzae Linnaeus (Coleoptera: Curculionidae) on sorghum grains

Contact Info

Product

Resources

About