Purification and some properties of pure Cochliobolus lunatus fibrinolytic Enzyme

Abstract

Purification of partially purified fibrinolytic enzyme was attempted by chromatography on DEAE-cellulose (D-52) column. The results indicated the resolution of three protein components and one minor component. It was shown that the first component was the major of the applied sample. Examination of fibrinolytic activity of the different fractions of components one and two indicated that only the first component possessed fibrinolytic activity. Fibrinolytic activity of the applied sample was completely recovered by the first enzyme component, and the most active fraction of this enzyme component showed 3.3-fold purification. The pure fibrinolytic enzyme was relatively more stable at pH 6.98, which was also optimal for its activity. After heating the enzyme solution (pH = 6.98) at 55 and 60 degrees C for 15 min, the enzyme still retained 34.7 and 17.3% of its original activity, respectively. Zinc ions partially inhibited the enzyme. Copper ions activated the enzyme. Iodine partially inhibited the fungal fibrinolytic enzyme at a final concentration of 10(-4)M; at 10(-2)M complete inactivation was brought about. The p-chloromercuribenzoate at a final concentration of 10(-2)M brought about partial inhibition whereby the enzyme lost about 33% of its original activity. Reduced glutathione brought about activation of the enzyme, while trypsin inhibitor did not show any effect on enzyme activity.

Green synthesis of silver nanoparticles from aqueous extract of Ziziphora clinopodioides Lam and evaluation of their bio‐activities under in vitro and in vivo conditions

“…It should be noted that because of the SPR characteristic, metallic silver nanocrystals indicated an optical absorption peak at roughly 3 keV. [9] Finally, the AFM 2D and 3D topographical images of synthesized AgNPs are shown in Figure 3F (a and b), respectively. It is clear from AFM images that AgNPs exhibited a uniform particle distribution and spherical shape with an average diameter around 30 nm.…”

“…Previously, many researchers have revealed that herbal nanoparticles similar to herbal extracts do not exhibit cytotoxicity properties. [9,10]…”

“…In the previous studies, scientists have indicated that herbal nanoparticles have excellent antioxidant effects. [9,10]…”

“…[7,8] In this regard, silver nanoparticles have a special place, because they have been shown to have anticancer properties against several cell lines such as human breast adenocarcinoma cells (SK-BR-3), human breast adenocarcinoma (MDA-MB-231), human breast adenocarcinoma (MCF-7), human cervical adenocarcinoma cells (HeLa), human lung carcinoma cells (A549), human chronic myelogenous cells, colon cancer cells (HCT-116), human colorectal adenocarcinoma cells, and human liver cancer cells (HepG2). [9] In the previous study, silver nanoparticles prepared using plants were used for their antibacterial activities against Pseudomonas aeruginosa, Escherichia coli O157:H7, Salmonella typhimurium, Bacillus subtilis, Staphylococcus aureus, and Streptococcus pneumonia; and antifungal potentials against Candida glabrata, Candida albicans, Candida krusei, and Candida guilliermondii; antioxidant properties against free radicals such as DPPH. They did not exhibit any cytotoxicity effect against human umbilical vein endothelial cells (HUVECs).…”

Effect of gold nanoparticles synthesized using the aqueous extract ofSatureja hortensisleaf on enhancing the shelf life and removingEscherichia coliO157:H7 andListeria monocytogenesin minced camel's meat: The role of nanotechnology in the food industry

“…They have combined nanotechnology with medicine to synthesize such supplements. [16] Among the metallic nanoparticles, the place of gold nanoparticles synthesized by plants is very special. Bioreduction of AuNPs is obtained via either plant tissues or microorganisms.…”