Purification and some properties of Clostridium botulinum type-E toxin

A Consideration to Immune Doses of Staphylococcal Enterotoxin B to Rabbits

“…Since the molecular weight of C. botulinum type E progenitor toxin is 350,000 daltons. (Kitamura et al, 1968), 10 times as high as that of staphylococcal enterotoxin, it is. probable that the amount of antigen causing immunological paralysis depends uponn the molecular number rather than the total quantity of the antigen injected primarily.…”

Responses of Clostridium Botulinum Type B and E Progenitor Toxins to Some Clostridial Sulfhydryl-Dependent Proteases

“…Two toxin peaks were eluted as reported by Kozaki et al (1974); the first one (L toxin) at or near the void volume of the column and the retarded one (M toxin) at the same position as that for eluting type E progenitor toxin (12S). Type E progenitor toxin was purified by the method reported by Kitamura, Sakaguchi and Sakaguchi (1968).…”

Separation of Clostridium Botulinum Type a Derivative Toxin Into Two Fragments

“…The molecule of the progenitor toxin of Clostridium botulinum types A through F is composed of two components, toxic and nontoxic (Iwasaki and Sakaguchi, 1978;Kitamura et al, 1968;Kozaki et al, 1974;Lowenthal and Lamanna, 1953;Miyazaki et al, 1977;Ohishi and Sakaguchi, 1975;Sugii and Sakaguchi, 1975). The toxic component or derivative toxin is made up of two polypeptide fragments with molecular weights of about 100,000 and 50,000 (Beers and Reich, 1968;DasGupta and Sugiyama, 1972;Miyazaki et al, 1977;Syuto and Kubo, 1977;Yang and Sugiyama, 1975).…”