Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes

Lin, Na; Mo, Xiaoli; Yang, Yang; Zhang, Hong

doi:10.1007/s10719-016-9759-y

Cited by 17 publications

(9 citation statements)

References 36 publications

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“…Skipjack tuna (Katsuwonus pelamis) is the main resource of canned fish and shares of more than 70% of tuna catches (3,26,27). Many researches have utilized by-products of skipjack tuna to produce some functional components, such as collagens and gelatins (28,29), BPs (9,21,30), oil and lipid (11,31), and multiple sulfated polysaccharides (32). Roe is one of the main by-products of skipjack tuna and is rich in crude proteins with the content of 21.00 ± 0.06% of tuna roe (on a wet weight basis) (33).…”

Section: Introductionmentioning

confidence: 99%

Twelve Antioxidant Peptides From Protein Hydrolysate of Skipjack Tuna (Katsuwonus pelamis) Roe Prepared by Flavourzyme: Purification, Sequence Identification, and Activity Evaluation

Wang

et al. 2022

Front. Nutr.

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For using aquatic by-products to manufacture high-value products, Skipjack tuna (Katsuwonus pelamis) roes were degreased, pretreated with microwave, and hydrolyzed using five proteases. The protein hydrolysate (TRPH) generated using Flavourzyme displayed the strongest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Twelve antioxidative peptides were prepared from TRPH by ultrafiltration and chromatography methods and determined to be SGE, VDTR, AEM, QDHKA, TVM, QEAE, YEA, VEP, AEHNH, QEP, QAEP, and YVM with molecular weights of 291.24, 489.50, 349.41, 597.59, 349.44, 475.42, 381.36, 343.37, 606.58, 372.35, 443.42, and 411.49 Da, respectively. AEM, QDHKA, YEA, AEHNH, and YVM presented the strongest scavenging activity on DPPH radical (EC50 values of 0.250±0.035, 0.279±0.017, 0.233±0.012, 0.334±0.011, and 0.288±0.015 mg/ml, respectively), hydroxyl radical (EC50 values of 0.456±0.015, 0.536±0.021, 0.476 ± 0.051, 0.369 ± 0.052, and 0.413 ± 0.019 mg/ml, respectively), and superoxide anion free radical (EC50 values of 0.348 ± 0.018, 0.281 ± 0.013, 0.305 ± 0.022, 0.198 ± 0.011, and 0.425 ± 0.021 mg/ml, respectively). Moreover, AEM, QDHKA, YEA, AEHNH, and YVM presented high lipid peroxidation inhibition ability, Ferric-reducing power, and significant protective function on H2O2-induced Chang liver cells. Therefore, AEM, QDHKA, YEA, AEHNH, and YVM could be natural antioxidant ingredients used in pharmaceutical and functional products.

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Section: Introductionmentioning

confidence: 99%

Twelve Antioxidant Peptides From Protein Hydrolysate of Skipjack Tuna (Katsuwonus pelamis) Roe Prepared by Flavourzyme: Purification, Sequence Identification, and Activity Evaluation

Wang

et al. 2022

Front. Nutr.

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“…Where do these doping heteroatoms come from? Usually, tuna bones are mainly composed of collagen, chondroitin sulfate (CS), , protein, and HAP. Among them are CS with abundant sulfonic acid groups and amine groups, and HAP with phosphate groups, which leads to heteroatom doping of TBCs.…”

Section: Results and Discussionmentioning

confidence: 99%

Fibrous Bio-Carbon Foams: A New Material for Lithium-Ion Hybrid Supercapacitors with Ultrahigh Integrated Energy/Power Density and Ultralong Cycle Life

Shan

Cui

Liu

et al. 2018

ACS Sustainable Chem. Eng.

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A naturally fibrous carbon foam is fabricated by employing fish bones as raw materials. Differing to carbon foams in previous reports, numerous unwoven and crosslinked carbon nanofibers were found to construct the whole foam, exhibiting a hierarchical porosity with interconnected channels and multisize pores. In the meantime, on the basis of the rich organic−inorganic components of fish bones, an N-S-P-O codoping was achieved in carbon foams. Benefiting from the synergistic effects of hierarchically porous fibrous foam and multiple heteroatom doping, the as-obtained samples were employed both as anode and cathode materials in lithium ion hybrid supercapacitors (LIHC), which delivered a superior energy density of 131 Wh kg −1 in 0−4.0 V. Significantly, at an impressive power density of 62000 W kg −1 , the energy density of this device can still reach an ultrahigh energy density of 72 Wh kg −1 , which presents a state of the art supercapacitors. More importantly, continuously being charged/discharged at a high current density of 5 A g −1 for 20000 cycles, the LIHC device can still retain a high energy density of 79 Wh kg −1 with 82% retention, demonstrating the promising prospect of heteroatom doped fibrous carbon foam in high-performance hybrid supercapacitors.

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“…The Lophius litulon (monkfish) are mainly distributed in the north-east Atlantic and live in a wide bathymetric range from shoal to seafloor of at least 1,000 m (Laurenson & Priede, 2005). As an abyssal fish with high nutrition and active substance content, the Lophius litulon have been wildly used for food and nutrition, including as a source bioactive substances and biological material (Lin et al, 2017;Stollewerk et al, 2014;Swart et al, 2019;Vázquez et al, 2020). The fish by-products from Lophius litulon processing could reach about 60% of the total weight with the liver occupying about 10%.…”

Section: Introductionmentioning

confidence: 99%

Optimization of fish oil extraction from Lophius litulon liver and fatty acid composition analysis

Chin¹,

Liu²,

Zhou³

et al. 2022

Fish Aquat Sci

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The Lophius litulon liver was used as raw material for the extraction of fish oil via various extraction methods. The extraction rate by water extraction, potassium hydroxide (KOH) hydrolysis and protease hydrolysis were compared and the results revealed the protease hydrolysis extraction had a higher extraction rate with good protein-lipid separation as observed by optical microscope. Furthermore, subsequent experiments determined neutrase to be the best hydrolytic enzyme in terms of extraction rate and cost. The extraction conditions of neutrase hydrolysis were optimized by single-factor experiment and response surface analysis, and the optimal extraction rate was 58.40 ± 0.25% with the following conditions: enzyme concentration 2,000 IU/g, extraction time 1.0 h, liquid-solid ratio 1.95:1, extraction temperature 40.5℃ and pH 6.5. The fatty acids composition in fish oil from optimized extraction condition was composed of 19.75% saturated fatty acids and 80.25% unsaturated fatty acids. The content of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were 8.06% and 1.19%, respectively, with the ratio (6.77:1) surpassed to the recommendation in current researches (5:1). The results in this study suggest protease treatment is an efficient method for high-quality fish oil extraction from Lophius litulon liver with a satisfactory ratio of DHA and EPA.

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Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes

Cited by 17 publications

References 36 publications

Twelve Antioxidant Peptides From Protein Hydrolysate of Skipjack Tuna (Katsuwonus pelamis) Roe Prepared by Flavourzyme: Purification, Sequence Identification, and Activity Evaluation

Twelve Antioxidant Peptides From Protein Hydrolysate of Skipjack Tuna (Katsuwonus pelamis) Roe Prepared by Flavourzyme: Purification, Sequence Identification, and Activity Evaluation

Fibrous Bio-Carbon Foams: A New Material for Lithium-Ion Hybrid Supercapacitors with Ultrahigh Integrated Energy/Power Density and Ultralong Cycle Life

Optimization of fish oil extraction from Lophius litulon liver and fatty acid composition analysis

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