Purification and Properties of β-Galactosidases fromBacillus circulans

Cloning and Characterization of the Gene Encoding a Novelβ-Galactosidase fromBacillus circulans

“…three fi-gals were also present in Biolacta N5 ( Fig. 1 lane 5) and were thus thought to be the fi-gal-l and -2 reported by Mozaffar et al 7) The third, that migrated fastest among the three, was found to be a novel fi-gal not previously reported.…”

Effect of Galactooligosaccharides on Calcium Absorption and Preventing Bone Loss in Ovariectomized Rats

“…Galactooligosaccharides (GOS) were prepared from lactose by transgalactosylation of fJ-D-galactosidases derived from Bacillus circulans 16 ) and subsequent removal of monosaccharides and lactose by using a column filled with a sodium-form cation-exchange resin. 1 7) Table I shows the sugar composition of GOS, whose main components were the trisaccharide, GalfJ(1-4)GalfJ(1-4)Glc, and tetrasaccharide, GalfJ(1-4)GalfJ(1-4 )GalfJ(l-4 )Glc.…”

Causes of the Production of Multiple Forms of β-Galactosidase byBacillus circulans

“…Previously, Nakanishi (one of the authors of this study), Mozaffar, and co-workers 12,13) isolated and purified two -galactosidases, -galactosidase-1, with an estimated molecular mass of approximately 240 kDa, and -galactosidase-2, with a molecular mass of 160 kDa by a commercial enzyme preparation produced by B. circulans (Biolacta Ò , Daiwa Kasei, Shiga, Japan), originally from B. circulans ATCC 31382. They reported that -galactosidase-1 showed very low transgalactosylation activity, while -galactosidase-2 had the highest level of transgalactosylation activity among a number of enzymes isolated.…”

“…GOS are important prebiotics that enhance the proliferation of intestinal bifidobacteria and lactobacilli in the colon, which in turn promote human health by protecting individuals against infection, reducing the accumulation of harmful bacteria/toxic compounds, and facilitating the normal function of the gut. [8][9][10][11] Because of this, a number of studies have reported attempts to isolate a -galactosidase with high transgalactosylation activity from various microorganisms, [4][5][6][7][8] including Bacillus circulans, 12,13) Bifidobacterium adolescentis, 14) Lactobacillus reuteri, 15,16) and others. [17][18][19][20] Furthermore, the genes that encode -galactosidases from bacteria, including B. circulans, 21) Bifidobacterium bifidum [22][23][24][25] / Bifidobacterium infantis, 22,[26][27][28] L. reuteri, 29) L. plantarum, 30) and others, 20,31) have been cloned and sequenced, and the enzymes have been overexpressed in heterologous cells.…”