Purification and properties of xylanase from the thermophilic fungus, Humicola lanuginosa (Griffon and Maublanc) Bunce

Anand, Lalitha; Krishnamurthy, S.; Vithayathil, Paul J.

doi:10.1016/0003-9861(90)90757-p

Cited by 56 publications

(26 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Paper of inferior quality was an excellent carbon source and inducer for xylanase in Thermoascus aurantiacus (139), Humicola lanuginosa (13,15), and Paecilomyces varioti (144). In Melanocarpus albomyces (156) and Thermomyces lanuginosus (205), xylose, the pentosan unit of xylan, could also induce xylanase.…”

Section: Xylanasementioning

confidence: 99%

See 1 more Smart Citation

Thermophilic Fungi: Their Physiology and Enzymes

Maheshwari

Bharadwaj

Bhat

2000

Microbiol Mol Biol Rev

648

380

View full text Add to dashboard Cite

SUMMARY Thermophilic fungi are a small assemblage in mycota that have a minimum temperature of growth at or above 20°C and a maximum temperature of growth extending up to 60 to 62°C. As the only representatives of eukaryotic organisms that can grow at temperatures above 45°C, the thermophilic fungi are valuable experimental systems for investigations of mechanisms that allow growth at moderately high temperature yet limit their growth beyond 60 to 62°C. Although widespread in terrestrial habitats, they have remained underexplored compared to thermophilic species of eubacteria and archaea. However, thermophilic fungi are potential sources of enzymes with scientific and commercial interests. This review, for the first time, compiles information on the physiology and enzymes of thermophilic fungi. Thermophilic fungi can be grown in minimal media with metabolic rates and growth yields comparable to those of mesophilic fungi. Studies of their growth kinetics, respiration, mixed-substrate utilization, nutrient uptake, and protein breakdown rate have provided some basic information not only on thermophilic fungi but also on filamentous fungi in general. Some species have the ability to grow at ambient temperatures if cultures are initiated with germinated spores or mycelial inoculum or if a nutritionally rich medium is used. Thermophilic fungi have a powerful ability to degrade polysaccharide constituents of biomass. The properties of their enzymes show differences not only among species but also among strains of the same species. Their extracellular enzymes display temperature optima for activity that are close to or above the optimum temperature for the growth of organism and, in general, are more heat stable than those of the mesophilic fungi. Some extracellular enzymes from thermophilic fungi are being produced commercially, and a few others have commercial prospects. Genes of thermophilic fungi encoding lipase, protease, xylanase, and cellulase have been cloned and overexpressed in heterologous fungi, and pure crystalline proteins have been obtained for elucidation of the mechanisms of their intrinsic thermostability and catalysis. By contrast, the thermal stability of the few intracellular enzymes that have been purified is comparable to or, in some cases, lower than that of enzymes from the mesophilic fungi. Although rigorous data are lacking, it appears that eukaryotic thermophily involves several mechanisms of stabilization of enzymes or optimization of their activity, with different mechanisms operating for different enzymes.

show abstract

Section: Xylanasementioning

confidence: 99%

“…However, the lyophilized xylanase of H. lanuginosa was inactivated after 2 months at Ϫ20°C, although the purified enzyme in solution did not lose activity (13). The lyophilized protein was resolved by gel filtration chromatography into a highmolecular-weight inactive protein and minor active proteins.…”

Section: Xylanasementioning

confidence: 99%

Thermophilic Fungi: Their Physiology and Enzymes

Maheshwari

Bharadwaj

Bhat

2000

Microbiol Mol Biol Rev

648

380

View full text Add to dashboard Cite

show abstract

“…Além disso, é necessário que o preparado enzimático seja livre de celulases, para evitar o ataque às fibras de celulose, tenha xilanases muito ativas, para reduzir o custo, e com baixo peso molecular, para facilitar sua difusão na polpa [126][127][128] . As xilanases comerciais como as Pulpzyme e Cartazyme não são termoestáveis o suficiente (atividade ótima 50-60 °C e mantêm 50% de sua atividade por 15 min a 55 °C) para evitar a necessidade de um resfriamento da polpa após o tratamento alcalino 44 .…”

Section: Xilanases Termoestáveisunclassified

Enzimas termoestáveis: fontes, produção e aplicação industrial

et al. 2007

View full text Add to dashboard Cite

“…For it, they require carbon substance as a source of energy and nitrogen substance to build protein and another essential compound. Further, a group of enzymes can be secreted simultaneously to target multiple nutrients (Chaturvedi et al, 2013;Anand et al, 1990). Keratinase is produced by various bacteria, actinomycetes and fungi in an optimum range of physical parameters (Farag and Hassan, 2004;Thys et al, 2004;Anbu et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Fungi produce proteases a group of proteinases and peptidases with varying itself nature basis on species (Chaturvedi et al, 2013;Anand et al, 1990). In the present study, the keratin degradation by keratinophilic fungal species (Chrysosporium tropicum and Arthoderma multifidium) was evaluated using modified feather degradation medium (K 2 HPO 4 -1.25 g; MgSO 4 .7H 2 O-0.025 g; CaCL 2 -0.02 g; FeSO 4 .7H 2 O-0.015 g; ZnSO 4 .7H 2 O-0.005 g; pH 7.0±0.2) for estimation of keratinolytic potential.…”

Section: Screen Out the Keratinolytic Potential Of Identified Keratinmentioning

confidence: 99%

Evaluation of Keratinolytic Activity Succeeds by Keratinophilic Fungi in Jaipur, India

Sharma¹,

Sharma²,

Seth³

2017

American Journal of Applied Sciences

View full text Add to dashboard Cite

Earth has innate background for fungi that cover individual kingdom since evolution. The keratinophilic fungi are allied moulds that produce the keratinase enzyme to degrade the keratinous materials in or on the soil. Keratinous materials are insoluble and resistant to degradation by common proteinase enzymes. It is important to study the microorganism producers of such enzymes for use in the biotechnology industry. In order to present study, two isolates of fungi were evaluated to determine if they had the ability to degrade keratin as nutrient substrate. They were grown in an inundated culture medium containing poultry feathers. Among species, best keratin substrate degradation activity as well as keratinase enzyme activity was recorded in Arthoderma multifidium (KU578107) followed by Chrysosporium tropicum (KU578108) gradually leading manner day by days.

show abstract

Purification and properties of xylanase from the thermophilic fungus, Humicola lanuginosa (Griffon and Maublanc) Bunce

Cited by 56 publications

References 35 publications

Thermophilic Fungi: Their Physiology and Enzymes

Thermophilic Fungi: Their Physiology and Enzymes

Enzimas termoestáveis: fontes, produção e aplicação industrial

Evaluation of Keratinolytic Activity Succeeds by Keratinophilic Fungi in Jaipur, India

Contact Info

Product

Resources

About