Purification and properties of Sandercyanin, a blue protein secreted in the mucus of blue forms of walleye, Sander vitreus

Yu, Chung Huang; Ferraro, D.J.; Ramaswamy, S.; Schmitz, Mark H.; Schaefer, Wayne F.; Gibson, David T.

doi:10.1007/s10641-007-9252-3

Cited by 12 publications

(18 citation statements)

References 15 publications

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“…Yu et al (1) have previously reported the purification of the native blue protein from walleye mucus and the partial protein sequence of Sandercyanin, which suggested that it belongs to the lipocalin family of proteins (20,21). They also showed that BLA is noncovalently bound to the protein.…”

Section: Resultsmentioning

confidence: 98%

“…Native Sandercyanin was extracted and purified from the mucus of blue forms of walleye from Northwest Ontario by chromatographic techniques as described previously (1). A putative amino acid sequence of the protein was determined from crystal structure of the native protein, confirmed, and corrected after partial genome sequencing of blue walleye.…”

Section: Methodsmentioning

confidence: 99%

“…We prepared DNA and carried out whole-genome sequencing experiments of the walleye. We determined the full-length gene sequence of the blue protein by partial assembly of the whole genome based on mapping of known internal peptides (1). The resulting gene sequence encoded for a secretion signal peptide of 19 amino acids in the N terminus of the protein.…”

Section: Significancementioning

confidence: 99%

“…However, a few biliverdin-binding lipocalins have been discussed in literature (1). These are known to bind biliverdin IX gamma isoform as the chromophore and impart blue color.…”

Section: Crystal Structures Of Apo-and Holo-sandercyanin Reveal Molecmentioning

confidence: 99%

“…Sandercyanin | walleye | blue protein | red fluorescent protein | UV radiation S andercyanin bound to biliverdin IXα is a blue colored proteinligand complex isolated from the skin mucus of walleye (Sander vitreus), a commercial and sport fish common in North America (1,2). Walleye are known to be sensitive to light and prefer being in the shade.…”

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confidence: 99%

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Blue protein with red fluorescence

Ghosh

Ferraro

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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The walleye (Sander vitreus) is a golden yellow fish that inhabits the Northern American lakes. The recent sightings of the blue walleye and the correlation of its sighting to possible increased UV radiation have been proposed earlier. The underlying molecular basis of its adaptation to increased UV radiation is the presence of a protein (Sandercyanin)-ligand complex in the mucus of walleyes. Degradation of heme by UV radiation results in the formation of Biliverdin IXα (BLA), the chromophore bound to Sandercyanin. We show that Sandercyanin is a monomeric protein that forms stable homotetramers on addition of BLA to the protein. A structure of the Sandercyanin-BLA complex, purified from the fish mucus, reveals a glycosylated protein with a lipocalin fold. This protein-ligand complex absorbs light in the UV region (λ max of 375 nm) and upon excitation at this wavelength emits in the red region (λ max of 675 nm). Unlike all other known biliverdin-bound fluorescent proteins, the chromophore is noncovalently bound to the protein. We provide here a molecular rationale for the observed spectral properties of Sandercyanin.Sandercyanin | walleye | blue protein | red fluorescent protein | UV radiation

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Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Significancementioning

confidence: 99%

“…However, a few biliverdin-binding lipocalins have been discussed in literature (1). These are known to bind biliverdin IX gamma isoform as the chromophore and impart blue color.…”

Section: Crystal Structures Of Apo-and Holo-sandercyanin Reveal Molecmentioning

confidence: 99%

mentioning

confidence: 99%

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