Purification and properties of nuclear and cytoplasmic deoxyribonucleic acidlike ribonucleic acid from Ehrlich ascites cells

Characterization of rapidly labelled rat liver ribonucleic acid showing high affinity for columns of methylated albumin on kieselguhr

“…The results of MAK-column chromatography of rapidly labelled RNA from rat and mouse liver as well as that from Saccharomyce8 cereviaiae show that a great part ofthe labelled RNA remains tightly bound to the column and can only be recovered with M-ammonia and not by eluting with a sodium chloride gradient. These results agree with the findings of Ellem &Sheridan (1964) andEllem (1966) for L-cell RNA, of Lingrel (1967) for bonemarrow RNA, of Roberts & Quinlivan (1969) for Ehrlich ascites-cell RNA and of Billing, Inglis & Smellie (1969) for rat liver RNA. The present results point to the caution needed in interpreting MAK-column chromatographs in which the alkali elution step has not been taken.…”

Characterization and poly(adenylic acid) content of Ehrlich ascites cell ribonucleic acids fractionated on unmodified cellulose columns

“…The cytoplasmic unbound fraction contained peaks of 28S and 18S rRNA and 4S tRNA. The bound fractions of both nuclear and cytoplasmic RNAs produced heterogeneous sedimentation patterns similar to those previously reported for hnRNA and hcRNA from ascites cells Edmonds and Caramela, 1969;Roberts and Quinlivan, 1969;Lee et al, 1971). The mean sedimentation values of the bound fractions are approximately 18 S for the cytoplasmic RNA and 32 S for the nuclear RNA.…”

“…The bound RNAs had relatively high specific activities and contained [32P]nucleotide compositions very different from the unbound fractions (Table I). The nucleotide compositions of the bound fractions were characteristic of hRNA (Edmonds and Caramela, 1969); the nuclear fraction was high in U and the cytoplasmic fraction high in A, as previously determined for hnRNA and hcRNA from ascites cells (Roberts and Quinlivan, 1969). The unbound fractions contained RNAs rich in G + C. The high G content and low A content of the nuclear unbound fraction is typical of ascites cell rpRNA (Roberts and D'Ari, 1968) " Calculated as per cent of total cytoplasmic or nuclear RNA that was bound or unbound to cellulose columns.…”

“…The nuclear RNA in these experiments (ascites cells labeled with H32P042-for 3 hr) represented 10% of the A^o units and 48% of the cpm found in the total cellular RNA. plasmic unbound fraction reflects a mixture of rRNA and tRNA (Roberts and Quinlivan, 1969).…”

Characterization of messenger-like ribonucleic acid from Saccharomyces cerevisiae by the use of chromatography on methylated albumin–kieselguhr

“…However, a large portion (about 80%) of the rapidly labelled RNA, which is DNA-like in base composition and has a sedimentation profile similar to polyribosomal mRNA, is tenaciously bound to the column (Ellem, 1966;Ewing & Cherry, 1967;Lingrel, 1967;Ellem & Rhode, 1968). This fraction, termed TD-RNA, can be eluted with a dilute solution of sodium dodecyl sulphate (Roberts & Quinlivan, 1969;Ellem, 1966). In this paper an analogous fraction of RNA from yeast was tested with regard to its base composition, sedimentation properties and labelling kinetics.…”