Characterization of messenger-like ribonucleic acid from <i>Saccharomyces cerevisiae</i> by the use of chromatography on methylated albumin–kieselguhr

Johnson, Roger W.

doi:10.1042/bj1190699

Cited by 9 publications

(3 citation statements)

References 27 publications

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“…The estimations of mRNA half-life based on the inhibition of transcription by DMC and EBR in S. cerevisiae are in agreement with those derived from polysome degradation in spheroplasts (15) and from the kinetics of appearance of newly synthesized RNA on spheroplast polysomes (13). Decay rates of tenaciously bound DNA-like RNA isolated from methylated albumin kieselguhr columns have been measured after a uracil pulse chase and half-life estimations ranging from 15 to 20 min were observed (16), and were in good agreement with our estimations. Both the present analysis and that based on decay of ipolysomes in mutant ts-136 as the nonpermissive temperature (15) measure essentially the functional aspect of mRNA.…”

Section: Discussionsupporting

confidence: 79%

Inhibitors of Ribonucleic Acid Synthesis in Saccharomyces cerevisiae : Decay Rate of Messenger Ribonucleic Acid

Tønnesen

Friesen

1973

J Bacteriol

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Daunomycin and ethidium bromide, two deoxyribonucleic acid-intercalating drugs, inhibit ribonucleic acid (RNA) and protein synthesis in Saccharomyces cerevisiae . Both agents rapidly curtail uptake of radioactive adenine, whereas the kinetics of radioactive leucine uptake after drug addition are consistent with translation of a pool of exponentially decaying messenger RNA. Messenger RNA half-life determinations from these experiments gave identical results over a range of drug concentrations; this value is 21 ± 4 min at 30 C. In a temperature-sensitive mutant in which RNA synthesis is curtailed at the nonpermissive temperature, a similar half-life for messenger RNA decay is found both in the absence and in the presence of either drug. This indicates that at the concentrations used in this study, neither daunomycin nor ethidium bromide has an appreciable direct effect on translation and do not increase the lability of messenger RNA.

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Section: Discussionsupporting

confidence: 79%

Inhibitors of Ribonucleic Acid Synthesis in Saccharomyces cerevisiae : Decay Rate of Messenger Ribonucleic Acid

Tønnesen

Friesen

1973

J Bacteriol

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“…The xRNA fraction is probably not mRNA since a more polydisperse distribution might, be expected, such as for ml~NA, 12 to 23S, from Saccharomyccs carlsbergensis (Hartlief and Koningsberger, 1968), and 12 to 20S, for S. cerevisiae (Johnson, 1970). There occur occasional reports of other than the established species of RNA, such as 23S and 19S from sea urchin eggs (Daigenault et al, 1970), but these do not exhibit the properties of the xRNA extracted from Rhizoctonia ,olaqi.…”

Section: Discussionmentioning

confidence: 82%

Synthesis of RNA with relation to aging inRhizoctonia solani

Davies

Gottlieb

1974

Arch. Microbiol.

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Summary. The RNA of the mycelium of Rhizoctonia solani was studied in cells of three different ages. Components corresponding to 25S and 18S, rRNA, and 4S + 5S RNA were present in approximately the same ratios in cells of each age. The rates of synthesis of all RNA species were markedly reduced in the ceils of the older mycelium, though turnover rates were not significantly altered.Various changes in metabolism which occur with increasing age in mycelial cells of the fungus Rhizoctonia solani have already been studied Gottlieb and Van Etten, 1966;Nicholas and Gottlieb, 1968;Obrig and Gottlieb, 1970;Van Etten et al., 1966). Associated with the aging process were decreased rates of respiration and protein synthesis. According to Obrig and Gottlieb (1970), the decreased protein synthesis was correlated with decreases in the activities of both the soluble enzymes and the ribosomes from the protein synthesizing mechanism in the older cells. Protein synthesis is in part dependent on the synthesis of RNA and conversely the formation of I%NA is dependent on protein synthesis. It has been demonstrated that cellular levels of RNA decrease with age in fungi (Gottlieb and Van Etten, 1966), in animals (Detwfler and Draper, 1962), and in higher plants (Kulaeva et al., 1967) and that the ratio of rRNA to tRNA changes with age in red blood cells (Burka, 1968). Also, Mainwaring (1968), using mouse prostate tissues, showed that a diminished rate of RNA, and in particular rRNA, synthesis accompanied aging in cells. Because of the intimate relationship of protein and RNA syntheses the formation of various species of RNA in aging cells was studied in the vegetative mycelium of R, solani.Abbreviations Used. rRNA = ribosomal ribonucleie acid; tRNA = transfer ribonucleic acid; mRNA = messenger ribonucleie acid; GA = glucose-asparagine.

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“…Finally, elution of the tenaciously bound RNA (mRNA) was accomplished by the passage of 50 ml of 0-1% (w/v) sodium dodecyl sulphate solution. 12 Isotope incorporation.-The incorporation of radioactive uracil into the intact yeast cells subjected to different periods of storage was performed at 30°C. A sample of 20 g of yeast was mixed with 100 ml buffer solution Graham & Caiger.10 increasing by approximately 20% compared with the original level.…”

Section: Pahkkinen Et DL I Nucleic Acids Of Stored Yeastmentioning

confidence: 99%

Effect of Storage on the Nucleic Acid Composition of Baker's Yeast*

Parkkinen

Oura

Suomalainen

1974

Journal of the Institute of Brewing

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General changes are described in the nucleic acid content of yeast stored for 20 days under extreme conditions (35°C).The total amount of nucleic acid increased in the first phase of 6-8 days storage. In the second phase, during which the yeast started to autolyse, the nucleic acid content diminished, first slowly and then more quickly. The quantity of acid-soluble nucleotides in creased with storage. The amount of dead yeast cells is low during the first period, but starts to increase quickly during the second period.Changes in the rRIMA, 5S RNA, tRNA, mRNA and DNA, fractionated on MAK columns, were investigated over a 20-day storage period. The contents of the RNA fractions increased initially during storage.The amount of rRNA attained its maximum value after 5 days, followed by DNA (7 days), then tRNA (10-12 days) and lastly 5S RNA; thereafter they all decreased. The mRNA fraction diminished rapidly: after only two days' storage the incorporation of labelled uracil into mRNA had declined by about 90%, showing that the ability to synthesize mRNA was soon lost. The short half-life of mRNA ensures its absence in yeast stored for longer periods.

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Characterization of messenger-like ribonucleic acid from Saccharomyces cerevisiae by the use of chromatography on methylated albumin–kieselguhr

Cited by 9 publications

References 27 publications

Inhibitors of Ribonucleic Acid Synthesis in Saccharomyces cerevisiae : Decay Rate of Messenger Ribonucleic Acid

Inhibitors of Ribonucleic Acid Synthesis in Saccharomyces cerevisiae : Decay Rate of Messenger Ribonucleic Acid

Synthesis of RNA with relation to aging inRhizoctonia solani

Effect of Storage on the Nucleic Acid Composition of Baker's Yeast*

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