Purification and Properties of Multiple Isoforms of a Novel Thiol Methyltransferase Involved in the Production of Volatile Sulfur Compounds from Brassica oleracea

Rice OsHOL1 and OsHOL2 proteins have S-adenosyl-L-methionine-dependent methyltransferase activities toward iodide ions

“…MCT, TMT, and HTMT proteins are also shown to possess SAM-dependent methyltransferase activity toward not only iodide ions, but also toward ions such as chloride, bromide, thiocyanate, and hydrosul de (Ni and Short Communication DOI: 10.5511/plantbiotechnology.12.0207a Abbreviations: GC-MS, Gas chromatography-mass spectrometry; HPLC, High performance liquid chromatography; MALDI-TOF-MS, Matrixassisted laser desorption/ionization time-of-ight mass spectrometry; SAH, S-Adenosyl-L-homocysteine; SAM, S-Adenosyl-L-methionine; SDS-PAGE, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 1999;Attieh et al 1995Attieh et al , 2000Itoh et al 2009). All kinetic analyses of plant HOL family proteins reported to date have been performed using proteins from Brassicales plants.…”

“…Biochemical analyses of the three AtHOL proteins showed that they have SAMdependent methyltransferase activities toward iodide ions as well as chloride, thiocyanate, and hydrosul de ions (Nagatoshi and Nakamura 2009). Plant proteins in which amino acid sequences showed high similarities to Arabidopsis HOL were classi ed as HOL family proteins, including independently characterized methyl chloride transferase (MCT) Hager 1998, 1999), thiol methyltransferase (TMT) (Attieh et al , 2000(Attieh et al , 2002, and halide/thiol methyltransferase (HTMT) (Itoh et al 2009). MCT, TMT, and HTMT proteins are also shown to possess SAM-dependent methyltransferase activity toward not only iodide ions, but also toward ions such as chloride, bromide, thiocyanate, and hydrosul de (Ni and Short Communication DOI: 10.5511/plantbiotechnology.12.0207a Abbreviations: GC-MS, Gas chromatography-mass spectrometry; HPLC, High performance liquid chromatography; MALDI-TOF-MS, Matrixassisted laser desorption/ionization time-of-ight mass spectrometry; SAH, S-Adenosyl-L-homocysteine; SAM, S-Adenosyl-L-methionine; SDS-PAGE, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis.…”

“…Biochemical analyses of the three AtHOL proteins showed that they have SAMdependent methyltransferase activities toward iodide ions as well as chloride, thiocyanate, and hydrosul de ions . Plant proteins in which amino acid sequences showed high similarities to Arabidopsis HOL were classi ed as HOL family proteins, including independently characterized methyl chloride transferase (MCT) Hager 1998, 1999), thiol methyltransferase (TMT) (Attieh et al , 2000(Attieh et al , 2002, and halide/thiol methyltransferase (HTMT) (Itoh et al 2009). MCT, TMT, and HTMT proteins are also shown to possess SAM-dependent methyltransferase activity toward not only iodide ions, but also toward ions such as chloride, bromide, thiocyanate, and hydrosul de (Ni and Hager 1999;Attieh et al 1995Attieh et al , 2000Itoh et al 2009).…”

Fluorescence-Based Bacterial Bioreporter for Specific Detection of Methyl Halide Emissions in the Environment

“…Natural sources of methyl halides are mainly living vegetation (7,8), wood rot fungi (9), dead plant material (10), biomass burning, oceans, and coastal waters (11). A thiol methyltransferase involved in the production of methyl halides was first isolated from the leaves of Brassica oleracea (12). In Arabidopsis thaliana, the S-adenosylmethionine-dependent methyltransferase gene HOL (harmless to ozone layer) was then shown to be involved in the production of methyl halides (13,14).…”

Characterization of three halide methyltransferases in Arabidopsis thaliana

“…It was reported that methyl chloride transferase (MCT) from Batis maritima (Wuosmaa and Hager 1990;Ni and Hager 1998) and thiol methyltransferases (TMT) from Brassica oleracea (Attieh et al 2000a;Attieh et al 2000b;Attieh et al 2002) possess S-adenosyl-L-methionine (SAM)-dependent methyltransferase activities and could synthesize methyl halide in vitro (Attieh et al 2000a;Wousmaa and Hager 1990). The hypotheses concerning the physiological meaning of these enzymes include their involvement in salt tolerance (Ni and Hager 1999) or in metabolizing glucosinolate hydrolysis products such as thiols and thiocyanate (Attieh et al 2000a).…”