Purification and properties of a non-histone chromatin protein with a molecular weight of 38,000 daltons specific for transformed rat cells

Nakayama, Tatsuo; Irikura, Maki; Setoguchi, Yoshiko; Matsuo, Hisayuki; Inoue, Hirokazu; Yutsudo, Masuo; Hakura, Akira

doi:10.1016/0006-291x(87)90529-8

Biochemical and Biophysical Research Communications

1987

DOI: 10.1016/0006-291x(87)90529-8

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Purification and properties of a non-histone chromatin protein with a molecular weight of 38,000 daltons specific for transformed rat cells

Tatsuo Nakayama

Maki Irikura

Yoshiko Setoguchi

et al.

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Cited by 2 publications

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“…To clarify the function of drs for cell transformation, we initially investigated the correlation between downregulation of drs mRNA and functional expression of the v-src gene. To perform this experiment, we used a rat F2408 cell clone (OS7-2) containing a temperature-sensitive mutant (OS122) of RSV (13,38,39,54). As shown in Fig.…”

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Suppression of v- src Transformation by the drs Gene

Inoue

Pan

Hakura

1998

J Virol

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Previously, we isolated a novel gene, drs, which was downregulated by retroviral oncogenes such as v-src and v-K-ras, from a cDNA library of primary rat embryo fibroblasts. Experiments using a temperature-sensitive mutant of the v-src gene indicated that downregulation of drsmRNA was dependent on functional expression of v-Src. In addition, expression of drs mRNA was also reduced by serum stimulation of G0-arrested normal rat fibroblast cells. To clarify the function of the drs gene in cell transformation and proliferation, we introduced drs linked to a potent promoter into a normal rat cell line, F2408, and examined the effect of ectopic expression of exogenous drs on the transformation by the v-src gene and growth properties. Cells expressing exogenous drs gene showed significantly decreased efficiency of transformation by v-src irrespective of functional expression of v-Src kinase, while the growth rate and G1/S progression of the cells were not suppressed by expression of exogenous drs gene, indicating thatdrs has the ability to suppress v-srctransformation without disturbing cell proliferation.

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“…S7-1 is an F2408 cell line transformed by the SR-D strain of RSV(23). OS7-2 is an F2408 cell line containing a temperature-sensitive mutant (OS122) of RSV(38,39,54). The cells were cultured in Dulbecco's modified Eagle's medium supplemented with 5% FCS (growth medium).…”

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Suppression of v- src Transformation by the drs Gene

Inoue

Pan

Hakura

1998

J Virol

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Isolation of a novel gene down‐regulated by v‐src

et al. 1996

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Purification and properties of a non-histone chromatin protein with a molecular weight of 38,000 daltons specific for transformed rat cells

Cited by 2 publications

References 8 publications

Suppression of v- src Transformation by the drs Gene

Suppression of v- src Transformation by the drs Gene

Isolation of a novel gene down‐regulated by v‐src

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