Purification and Physiological Effects of Human Urinary Erythropoietin*

Winkert, John W.; Gordon, Albert S.; Winkert, Elizabeth

doi:10.1111/j.2164-0947.1961.tb00757.x

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April 19651

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1962

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Cited by 11 publications

(1 citation statement)

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“…The GPF extracted from CE 1460 tumor tissue has several physicochemical characteristics in common with those reported by Lowy and Borsook (11) and Winkert et al (12) for erythropoietin. These include thermostability, pH stability, the (NH4)2SO4 concentration for "salting out," failure to dialyze, and nonabsorption by Sepha-dex G-75 dextran gel.…”

Section: April 1965supporting

confidence: 71%

Granulocytosis-Promoting Extract of Mouse Tumor Tissue: Partial Purification

Delmonte

Liebelt

1965

Science

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A granulocytosis-promoting extract of mouse tumor tissue was partially purified by differential precipitation with cold 20- to 30-percent ethanol at pH 6.5, "salting out" with 50- to 75-percent saturated ammonium sulfate, and filtration through Sephadex G-75 dextran gel. In intact mice, single doses of the extract (at least 12 micrograms per gram of body weight) induced up to a 30-fold increase in circulating granulocytes and a two- to threefold decrease in circulating mononuclear cells within 3 to 7 hours of intravenous injection.

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Section: April 1965supporting

confidence: 71%